Mass Spectrometric Imaging for Biomedical Tissue Analysis

Chughtai, Kamila; Heeren, Ron M. A.

doi:10.1021/cr100012c

Cited by 585 publications

(649 citation statements)

References 336 publications

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“…Imaging mass spectrometry (IMS) [23][24][25] is particularly well suited for investigating fingermarks because it combines suspect identification by visualizing the ridge pattern defined by the molecular images, and the chemical composition for further forensic evidence. Despite proven capabilities in fingermark analysis, for the detection and mapping of endogenous and exogenous markers, MALDI IMS critically relies on the nature of the matrix and its mode of deposition that may need tailoring to the chemistry of the interested species.…”

Section: Introductionmentioning

confidence: 99%

Development of Laser Desorption Imaging Mass Spectrometry Methods to Investigate the Molecular Composition of Latent Fingermarks

Lauzon

Dufresne

Chauhan

et al. 2015

J. Am. Soc. Mass Spectrom.

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Abstract. For a century, fingermark analysis has been one of the most important and common methods in forensic investigations. Modern chemical analysis technologies have added the potential to determine the molecular composition of fingermarks and possibly identify chemicals a suspect may have come into contact with. Improvements in analytical detection of the molecular composition of fingermarks is therefore of great importance. In this regard, matrix-assisted laser desorption ionization (MALDI) and laser desorption ionization (LDI) imaging mass spectrometry (IMS) have proven to be useful technologies for fingermark analysis. In these analyses, the choice of ionizing agent and its mode of deposition are critical steps for the identification of molecular markers. Here we propose two novel and complementary IMS approaches for endogenous and exogenous substance detection in fingermarks: sublimation of 2-mercaptobenzothiazol (2-MBT) matrix and silver sputtering.

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Section: Introductionmentioning

confidence: 99%

Development of Laser Desorption Imaging Mass Spectrometry Methods to Investigate the Molecular Composition of Latent Fingermarks

Lauzon

Dufresne

Chauhan

et al. 2015

J. Am. Soc. Mass Spectrom.

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“…In addition, Heeren and co-workers from Amolf Institute presented a new approach in MSI, microscope mode imaging, where secondary ions/molecules are detected by the combination of ion optics and position sensitive detector enabling lateral resolution of $600 nm. 16 This approach decouples effective lateral resolution from desorption and ionization spot size, so broad primary beam can be used. It has been recently demonstrated 17,18 that the use of MeV primary ions, instead of the keV energy ions that are used in conventional SIMS, significantly suppressed fragmentation and simultaneously enhanced the secondary ion yield, especially for higher mass molecules (100-1000 Da).…”

mentioning

confidence: 99%

Submicron mass spectrometry imaging of single cells by combined use of mega electron volt time-of-flight secondary ion mass spectrometry and scanning transmission ion microscopy

Siketić

Radović

Jakšić

et al. 2015

Applied Physics Letters

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Articles you may be interested in Two-dimensional and three-dimensional dynamic imaging of live biofilms in a microchannel by time-of-flight secondary ion mass spectrometry Comparison of the submicron particle analysis capabilities of Auger electron spectroscopy, time-of-flight secondary ion mass spectrometry, and scanning electron microscopy with energy dispersive x-ray spectroscopy for particles deposited on silicon wafers with 1 μm thick oxide layers

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“…Two important fields in which Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry (LA-ICP-MS) is experiencing growing demand are those of imaging/mapping, notably of biological targets [1][2][3][4][5][6] and the analysis of discrete smallvolume samples such as single cells [7][8][9][10][11] or nanoparticulate [12][13][14] . For example this technique has been used to investigate the etiology and pathogenesis of disease states arising from imbalances in metal homeostasis.…”

Section: Introductionmentioning

confidence: 99%

High-Speed, Integrated Ablation Cell and Dual Concentric Injector Plasma Torch for Laser Ablation-Inductively Coupled Plasma Mass Spectrometry

et al. 2015

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In recent years, laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS) has gained increasing importance for biological analysis, where ultratrace imaging at micrometer resolution is required. However, while undoubtedly a valuable research tool, the washout times and sensitivity of current technology have restricted its routine and clinical application. Long periods between sampling points are required to maintain adequate spatial resolution. Additionally, temporal signal dispersion reduces the signal-to-noise ratio, which is a particular concern when analyzing discrete samples, such as individual particles or cells. This paper describes a novel, two-volume laser ablation cell and integrated ICP torch designed to minimize aerosol dispersion for fast, efficient sample transport. The holistic design utilizes a short, continuous diameter fused silica conduit, which extends from the point of ablation, through the ICP torch, and into the base of the plasma. This arrangement removes the requirement for a dispersive component for argon addition, and helps to keep the sample on axis with the ICP cone orifice. Hence, deposition of sample on the cones is theoretically reduced with a resulting improvement in the absolute sensitivity (counts per unit mole). The system described here achieved washouts of 1.5, 3.2, and 4.9 ms for NIST 612 glass, at full width half, 10%, and 1% maximum, respectively, with an 8–14-fold improvement in absolute sensitivity, compared to a single volume ablation cell. To illustrate the benefits of this performance, the system was applied to a contemporary bioanalytical challenge, specifically the analysis of individual biological cells, demonstrating similar improvements in performance.

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Mass Spectrometric Imaging for Biomedical Tissue Analysis

Cited by 585 publications

References 336 publications

Development of Laser Desorption Imaging Mass Spectrometry Methods to Investigate the Molecular Composition of Latent Fingermarks

Development of Laser Desorption Imaging Mass Spectrometry Methods to Investigate the Molecular Composition of Latent Fingermarks

Submicron mass spectrometry imaging of single cells by combined use of mega electron volt time-of-flight secondary ion mass spectrometry and scanning transmission ion microscopy

High-Speed, Integrated Ablation Cell and Dual Concentric Injector Plasma Torch for Laser Ablation-Inductively Coupled Plasma Mass Spectrometry

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