Mass spectrometry based analytical approaches and pitfalls for toxicometabolomics of arsenic in mammals: A tutorial review

García-Barrera, Tamara; Rodríguez-Moro, Gema; Callejón‐Leblic, Belén; Arias-Borrego, Ana; Gómez-Ariza, José Luis

doi:10.1016/j.aca.2017.10.019

Cited by 14 publications

(15 citation statements)

References 162 publications

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“…One of the new applications of MS is the quantitative analysis of PEGylated pharmaceuticals in vivo, which can offer femtomolar sensitivity and excellent potential for the detection of various analytes from small molecules to high MW polymers [135–143]. Commonly used MS methods for the bioanalysis of PEGylated pharmaceuticals include inductively coupled plasma‐MS (ICP‐MS) and LC–MS/MS.…”

Section: Bioanalytical Methods For Polyethylene Glycols and Pegylatedmentioning

confidence: 99%

Recent advances in the bioanalytical methods of polyethylene glycols and PEGylated pharmaceuticals

Zhang

Song

et al. 2020

J of Separation Science

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Polyethylene glycols are synthetic polymers composed of repeating oxyethylene subunits, which have been known for non-toxic, non-immunogenic, non-antigenic, good solubility in water and therefore approved for pharmaceutical applications. Recently, attachment or amalgamation of polyethylene glycols to therapeutic small molecules, peptides, proteins, or nanoparticles has become a mature technology for the sake of improving their pharmacokinetic and pharmacological profiles, also referred to as PEGylation. By comparison, there are only a few PEGylated pharmaceuticals have been registered for further clinical trials and even less was approved for marketing. High failure rate of PEGylated pharmaceuticals in pre-clinical and clinical trials could be majorly attributed to their unclear pharmacokinetic behaviors. Therefore, the in vivo fate of the PEGylated pharmaceuticals for the various routes of administration needs to be thoroughly investigated An accurate in vivo pharmacological study thereof highly depends on the precise detection of polyethylene glycols as well as their fragments in biological matrixes. The goal of this review is to highlight the analytical methods that were developed and applied to evaluate the polyethylene glycols in pharmaceutical ingredients and excipients, which bring us closer to bridging

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Section: Bioanalytical Methods For Polyethylene Glycols and Pegylatedmentioning

confidence: 99%

Recent advances in the bioanalytical methods of polyethylene glycols and PEGylated pharmaceuticals

Zhang

Song

et al. 2020

J of Separation Science

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“…In recent years, it has been attracting more attention as a powerful tool for exploring the alteration of small molecules. Metabolomics is also applied to toxicology through the concept of “toxicometabolomics” [ 5 , 6 ]. Metabolome analysis provides an instantaneous snapshot of the physiology and alterations of chemical processes involving metabolites, small molecule substrates, intermediates, and products of metabolism in response to exposure to environmental contaminants (e.g., metals) [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolomic Alteration in the Plasma of Wild Rodents Environmentally Exposed to Lead: A Preliminary Study

Nakata

Eguchi

Nakayama

et al. 2022

IJERPH

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Lead poisoning is often considered a traditional disease; however, the specific mechanism of toxicity remains unclear. The study of Pb-induced alterations in cellular metabolic pathways is important to understand the biological response and disorders associated with environmental exposure to lead. Metabolomics studies have recently been paid considerable attention to understand in detail the biological response to lead exposure and the associated toxicity mechanisms. In the present study, wild rodents collected from an area contaminated with lead (N = 18) and a control area (N = 10) were investigated. This was the first ever experimental metabolomic study of wildlife exposed to lead in the field. While the levels of plasma phenylalanine and isoleucine were significantly higher in a lead-contaminated area versus the control area, hydroxybutyric acid was marginally significantly higher in the contaminated area, suggesting the possibility of enhancement of lipid metabolism. In the interregional least-absolute shrinkage and selection operator (lasso) regression model analysis, phenylalanine and isoleucine were identified as possible biomarkers, which is in agreement with the random forest model. In addition, in the random forest model, glutaric acid, glutamine, and hydroxybutyric acid were selected. In agreement with previous studies, enrichment analysis showed alterations in the urea cycle and ATP-binding cassette transporter pathways. Although regional rodent species bias was observed in this study, and the relatively small sample size should be taken into account, the present results are to some extent consistent with those of previous studies on humans and laboratory animals.

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“…Metabolomic approaches are mainly based on the use of two analytical platforms, nuclear-magnetic-resonance (NMR) spectroscopy and mass spectrometry (MS) (Dettmer et al 2007;Goldsmith et al 2010). In spite of it is destructive and present some interferences, mass spectrometry (MS), has the advantages of its high sensitivity and specificity, and that it is suitable for complex samples and allows obtaining quantitative results (García-Barrera et al 2018). Moreover, it is a suitable technique for molecular identification of unknown metabolites, especially tandem MS/MS experiments for fragment ion studies using direct infusion (Vuckovic 2012;García-Sevillano et al 2013a, 2014a.…”

Section: Introductionmentioning

confidence: 99%

“…Although this approach present important advantages (i.e. simplicity, sample throughput, absence of sample manipulation, detection capability) (Draper et al 2013;García-Barrera et al 2018), the complexity of the obtained mass spectra is high and isobaric interferences are usually present. To overcome this problem, MS can be used with a previous separation step such as high performance liquid chromatography (HPLC), capillary electrophoresis (CE), or gas chromatography (GC) after chemical derivatization.…”

Section: Introductionmentioning

confidence: 99%

Metabolic impairments, metal traffic, and dyshomeostasis caused by the antagonistic interaction of cadmium and selenium using organic and inorganic mass spectrometry

Rodríguez-Moro

Roldán

Baya-Arenas

et al. 2019

Environ Sci Pollut Res

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Cadmium (Cd) has become one of the most important environmental pollutants in the world, derived from natural and industrial sources, which is known to be accumulated in the human body, producing serious health effects. On the other hand, Selenium (Se) is an essential element for mammals, which is well known for its antagonistic interaction against Cd toxicity, such as the prevention of oxidative stress induced by this element. For this reason, the use of complementary analytical methods to study the homeostasis of metals, "traffic" between different organs and massive information about metabolites altered by the exposure is of great interest. To this end, a metabolomic workflow based on the use of direct infusion mass spectrometry (DIMS) and gas chromatography mass spectrometry (GC-MS) was applied in mice serum. On the other hand, metals homeostasis and traffic between different organs and serum of mice exposed to Cd and Se, have been evaluated by determining the concentration of metals by inductively coupled plasma mass spectrometry. This work, demonstrate for the first time that Cd exposure causes a decrease of all the elements studied in lung except itself. On the other hand, Se provokes As trafficking from metabolically less active organs (brain, lung and testes) to others with greater metabolic activity (kidney), which also facilitates its excretion. Moreover, when mice are only exposed to Se, it provokes the accumulation of almost all the elements in kidney, except Cd that increases also in liver and brain. However, when both elements are simultaneously administered, Se increases Cd concentration in all the organs except in serum and especially in testis. On the other hand, important metabolic alterations have been detected in the energy and amino acid metabolism, as well as degradation of phospholipidic membranes, and in free fatty acids. In summary, the results show the high potential of the combined use of organic and inorganic mass spectrometry to establish Cd and Se interaction, the biological impairments caused and to provide information about metals traffic and metabolomic changes in exposure experiments.

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Mass spectrometry based analytical approaches and pitfalls for toxicometabolomics of arsenic in mammals: A tutorial review

Cited by 14 publications

References 162 publications

Recent advances in the bioanalytical methods of polyethylene glycols and PEGylated pharmaceuticals

Recent advances in the bioanalytical methods of polyethylene glycols and PEGylated pharmaceuticals

Metabolomic Alteration in the Plasma of Wild Rodents Environmentally Exposed to Lead: A Preliminary Study

Metabolic impairments, metal traffic, and dyshomeostasis caused by the antagonistic interaction of cadmium and selenium using organic and inorganic mass spectrometry

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