Mass Spectrometry‐Based Metabolomics Toward Biological Function Analysis

Qiu, Shi; Wang, Wanying; Zhang, Aihua; Wang, Xijun

doi:10.1002/9783527835751.ch12

Cited by 5 publications

(4 citation statements)

References 55 publications

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“…Magnoflorine (Mag) has multiple pharmacological activities for the prevention and treatment of prostatitis. The promising metabolomics strategy has been used for reveling the metabolic biomarkers, ans efficacy mechanisms, as well as pharmacological targets for drugs ( Qiu et al, 2021 ; Ren et al, 2021 ; Zhang et al, 2021 ). In this work, metabolomics method was used to clarify the intervention of Mag against prostatitis and the biological mechanism.…”

Section: Discussionmentioning

confidence: 99%

Liquid Chromatograph-Mass Spectrometry-Based Non-targeted Metabolomics Discovery of Potential Endogenous Biomarkers Associated With Prostatitis Rats to Reveal the Effects of Magnoflorine

et al. 2021

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Magnoflorine (Mag) has multiple pharmacological activities for the prevention and treatment of prostatitis. However, its molecular mechanisms andpharmacological targets are not clear. In this study, the ultra-performance liquid tandem mass spectrometry-based metabolomics method was used to clarify the intervention of Mag against prostatitis and the biological mechanism. A total of 25 biomarkers associated with the prostatitis model were identified by metabolomics, and a number of metabolic pathways closely related to the model were obtained by MetPA analysis. After given Mag treatment, the results of each indicator were shown that Mag alkaloid could inhibit the development of prostatitis effectively. We found that Mag had regulative effects on potential biomarkers of prostatitis model, which can regulate them to the control group. Our results indicated that alkaloids have an effective intervention therapy for prostatitis, and five types of metabolic pathways closely related to prostatitis model were obtained, including phenylalanine, tyrosine and tryptophan biosynthesis, phenylalanine metabolism, tyrosine metabolism, arginine and proline metabolism, glycine, serine and threonine metabolism, alanine, aspartate and glutamate metabolism. This study has provided the basic experimental data for the development of Mag in the prevention and treatment of prostatitis.

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Section: Discussionmentioning

confidence: 99%

Liquid Chromatograph-Mass Spectrometry-Based Non-targeted Metabolomics Discovery of Potential Endogenous Biomarkers Associated With Prostatitis Rats to Reveal the Effects of Magnoflorine

et al. 2021

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“…The main task of metabolomics is to identify and quantify a wide range of metabolites [51]. Detection of specific metabolic changes can serve as an indicator of the development of various diseases, in particular, in diabetes mellitus [52] or in chronic obstructive pulmonary disease [53].…”

Section: E Molecular Genetic Methods For Identifying Microorganismsmentioning

confidence: 99%

Untitled

2023

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Вісник медичних і біологічних дослідженьТом 5, № 4. 2023 Редакційна колегія Головний редактор Лариса Федонюк Доктор медичних наук, професор, Тернопільський національний медичний університет імені І. Я. Горбачевського, Україна Відповідальний редактор Тарас П'ятковський Кандидат медичних наук, доцент, Тернопільський національний медичний університет імені І. Я. Горбачевського, Україна Відповідальний секретар Неля Гливка Кандидат біологічних наук, асистент, Тернопільський національний медичний університет імені І. Я. Горбачевського, Україна Національні члени редколегії Лоліта Воронцова Доктор медичних наук, професор, Запорізька медична академія післядипломної освіти Міністерства охорони здоров'я України, Україна Ігор Господарський Доктор медичних наук, професор, Тернопільський національний медичний університет імені І. Я. Горбачевського, Україна Надія Деміхова Доктор медичних наук, професор, Сумський державний університет, Україна Інна Криницька Доктор медичних наук, професор, Тернопільський національний медичний університет імені І. Я. Горбачевського, Україна Оксана Кущ Доктор біологічних наук, професор, Запорізький державний медичний університет, Україна Любов Лаповець Доктор медичних наук, професор, Львівський національний медичний університет імені Данила Галицького, Україна Петро Лихацький Доктор біологічних наук, професор, Тернопільський національний медичний університет імені І. Я. Горбачевського, Україна Людмила Мазур Кандидат медичних наук, доцент, Тернопільський національний медичний університет імені І. Я. Горбачевського, Україна Лілія Мартинюк Доктор медичних наук, професор, Тернопільський національний медичний університет імені І. Я. Горбачевського, Україна Марія Марущак Доктор медичних наук, професор, Тернопільський національний медичний університет імені І. Я. Горбачевського, Україна Марія Хара Доктор медичних наук, професор, Тернопільський національний медичний університет імені І. Я. Горбачевського, Україна Олександр Шідловський Доктор медичних наук, професор, Тернопільський національний медичний університет імені І. Я. Горбачевського, Україна Світлана Ястремська Доктор педагогічних наук, професор, Тернопільський національний медичний університет імені І. Я. Горбачевського, Україна Міжнародні члени редколегії Елізабет Бургес-Пінто Доктор медсестринства, Університет МакЕвана, Канада

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Section: E Molecular Genetic Methods For Identifying Microorganismsmentioning

confidence: 99%

Microbiological diagnostics: From traditional to molecular genetic methods: A literature review

Ivashko,

Burmei,

Yusko

et al. 2023

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The development of new and optimisation of known cultural and molecular genetic methods for accurate species identification of microorganisms is an urgent and practically necessary task that is receiving great attention from researchers. The purpose of this study was to analyse and systematise theoretical scientific data on methods of microbial identification and assess their main advantages and disadvantages. For this purpose, a systematic review of 53 randomised research papers published between 2018 and 2023 was conducted. The search for publications using the keywords “microbiome”, “microbiological diagnostics”, “identification of microorganisms”, “sequencing”, and “omics technologies” in the title or text of the research paper was carried out in Web of Science, Scopus, PubMed, and Google Scholar. The study provides generalised information on traditional and modern methods of microbial identification. It is established that the advantages of traditional diagnostic methods include the possibility of preserving the obtained microorganisms for further research, in particular, for determining their antibiotic sensitivity. Modern molecular genetic methods open up new possibilities for the accurate identification of microorganisms, including those that are difficult to incubate using traditional cultural methods. The use of these methods allows obtaining detailed data on the genetic structure and diversity of microorganisms, which is important in many fields, including microbiology, medicine, and ecology. However, molecular methods are more sensitive to contamination or errors in the sample collection and processing. Moreover, omics technologies (genomics, transcriptomics, proteomics, and metabolomics) open up new opportunities for studying cells at more complex levels of life organisation. In everyday clinical practice, the microbiological method of isolating microbial cultures remains the main one, as it is the only one that allows determining not only the cause of the infection but also the antibiotic sensitivity. However, cultural methods for studying fastidious microorganisms are quite limited. Thus, the results obtained contribute to the development of fast and accurate methods for identifying, classifying, and systematising microorganisms, which facilitates the processes of diagnosis and development of strategies for the control and treatment of infectious diseases in laboratories and clinical institutions

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Mass Spectrometry‐Based Metabolomics Toward Biological Function Analysis

Cited by 5 publications

References 55 publications

Liquid Chromatograph-Mass Spectrometry-Based Non-targeted Metabolomics Discovery of Potential Endogenous Biomarkers Associated With Prostatitis Rats to Reveal the Effects of Magnoflorine

Liquid Chromatograph-Mass Spectrometry-Based Non-targeted Metabolomics Discovery of Potential Endogenous Biomarkers Associated With Prostatitis Rats to Reveal the Effects of Magnoflorine

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Microbiological diagnostics: From traditional to molecular genetic methods: A literature review

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