Mass Spectrometry‐Based Methodologies for Pharmacokinetic Characterization of Antibody Drug Conjugate Candidates During Drug Development

New Frontiers in Drug Development Utilizing Desorption by Impulsive Vibrational Excitation for Sample Preparation, Tissue Imaging and Beyond

“…In addition, nonclinical pharmacological evaluation of the ADC needs to be performed to determine if drug components penetrate the tumor tissue efficiently and can be retained there at high and biologically active concentrations. Analytical techniques that provide information concerning the distribution within a specific target area of multiple components from ADC drugs are critical to optimize the efficacy of an ADC against targeted tumors in nonclinical pharmacological evaluation [15][16][17]. Although the tissue distribution of radiolabeled small molecules has been obtained by autoradiography, differentiation between a radiolabeled drug conjugated to an ADC and free radiolabeled drug released from the ADCs has been a challenging task due to the inherit nonspecificity nature of radioactivity detection [17], unless additional studies are conducted for either radiolabel small molecule payload or labeled antibody.…”