Mass Spectrometry-Based Microbial Metabolomics: Techniques, Analysis, and Applications

Baidoo, Edward E. K.; Benites, Veronica T.

doi:10.1007/978-1-4939-8757-3_2

Cited by 21 publications

(33 citation statements)

References 109 publications

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“…To get an accurate depiction of the metabolic processes occurring in a sampled biomass, proper quenching and extraction techniques are necessary. High efficiency metabolic quenching, or the cessation of all cellular activity through protein and nucleotide denaturation, enables accurate metabolite information to be collected . However, efficient quenching of some metabolites can be challenging due to their extremely short half‐lives or involvement in several metabolic reactions .…”

Section: Investigating the Gut Microbiome With Metabolomicsmentioning

confidence: 99%

“…Significant damage to the cell membranes of the gut microbiota should be avoided to prevent intracellular metabolite leakage . To date, the most efficient quenching methods immediately suspend the microbial culture in the quenching agent and remove biomass through low‐speed centrifugation or fast‐filtration . Extraction of microbial metabolites is then achieved by the addition of extraction buffer, with or without mechanical microbial cell lysis .…”

Section: Investigating the Gut Microbiome With Metabolomicsmentioning

confidence: 99%

“…Untargeted metabolomic studies aim to identify all detectable metabolites in a biological sample. The resulting data are then statistically analyzed for similarities or differences between sample types which leads to a reduction in the number of relevant metabolites identified . This analytical approach could be used to identify biomarkers of a particular physiological state, which could lead to new treatment targets for diseases.…”

Section: Investigating the Gut Microbiome With Metabolomicsmentioning

confidence: 99%

“…This analytical approach could be used to identify biomarkers of a particular physiological state, which could lead to new treatment targets for diseases. With untargeted metabolomics, the data acquisition systems used must have excellent resolution, reproducibility, high sensitivity, and a wide dynamic range . One disadvantage of untargeted metabolomics experiments is the long data extraction, curation, and analysis that must occur.…”

Section: Investigating the Gut Microbiome With Metabolomicsmentioning

confidence: 99%

“…Prior knowledge of the target metabolites is critical so external chemical calibration curves can be used to gain accurate quantitative data. Exceptional analytical reproducibility is of upmost importance with targeted metabolomics to differentiate variances found in biological samples and reduce the number of replicates required . The analytical method most frequently used for targeted analysis of fecal samples to date is LC‐MS using hybrid triple‐quadrupole‐linear ion trap (QTRAP) or triple quadrupole (QqQ) mass spectrometers with MRM .…”

Section: Investigating the Gut Microbiome With Metabolomicsmentioning

confidence: 99%

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Metaproteomic and Metabolomic Approaches for Characterizing the Gut Microbiome

et al. 2019

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The gut microbiome has been shown to play a significant role in human healthy and diseased states. The dynamic signaling that occurs between the host and microbiome is critical for the maintenance of host homeostasis. Analyzing the human microbiome with metaproteomics, metabolomics, and integrative multi-omics analyses can provide significant information on markers for healthy and diseased states, allowing for the eventual creation of microbiome-targeted treatments for diseases associated with dysbiosis. Metaproteomics enables functional activity information to be gained from the microbiome samples, while metabolomics provides insight into the overall metabolic states affecting/representing the host-microbiome interactions. Combining these functional -omic platforms together with microbiome composition profiling allows for a holistic overview on the functional and metabolic state of the microbiome and its influence on human health. Here the benefits of metaproteomics, metabolomics, and the integrative multi-omic approaches to investigating the gut microbiome in the context of human health and diseases are reviewed.Samples collected from the human gut contains microbiome and host proteins. These microbiome/host proteins directly represent the functional activity of the intestinal ecosystem and can be simultaneously measured by metaproteomics. Metaproteomics can also provide valuable information on the pathways and taxaspecific functions, which provides a more in-depth perspective for studying detailed mechanisms underlying host-microbiome

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Section: Investigating the Gut Microbiome With Metabolomicsmentioning

confidence: 99%

Section: Investigating the Gut Microbiome With Metabolomicsmentioning

confidence: 99%

Section: Investigating the Gut Microbiome With Metabolomicsmentioning

confidence: 99%

Section: Investigating the Gut Microbiome With Metabolomicsmentioning

confidence: 99%

Section: Investigating the Gut Microbiome With Metabolomicsmentioning

confidence: 99%

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