Mass spectrometry-based proteomics for the forensic identification of vomit traces

Pieri, Maria; Silvestre, Angela; Cicco, Maristella De; Mamone, Gianfranco; Capasso, Emanuele; Addeo, Francesco; Picariello, Gianluca

doi:10.1016/j.jprot.2019.103524

Cited by 12 publications

(4 citation statements)

References 22 publications

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“…The same is also true for sweat (section 7.1) [166,167] and vomit [168]. Forensically relevant solid tissues such as bone [130,134,135,[169][170][171][172][173], teeth (section 7.4) [174][175][176][177][178][179][180][181][182][183][184][185], hair (section 6.6) [9,[186][187][188][189][190][191][192], nail plate [139], and skin cells (section 7.1) [148,193,194], have also been thoroughly processed and analyzed for both forensic as well as clinical purposes.…”

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 99%

“…However, forensically useful information may also be expressed as ratios and relative abundances in comparison with other tissues and body fluids [154,232]. A number of untargeted proteomics studies have led to an extensive, somewhat overlapping list of biomarker targets for forensically relevant biological fluids including peripheral blood, vaginal/menstrual fluid, semen, urine, nasal secretion, vomit and saliva [154,161,162,165,168]. Overall, 44 fluid specific biomarkers were identified via several approaches (Table 1) [154,161,165,233].…”

Section: Discovery and Validation Of Body Fluid And Tissue Biomarkersmentioning

confidence: 99%

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Forensic proteomics

Parker

McKiernan

Legg

et al. 2021

Forensic Science International: Genetics

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Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 99%

Section: Discovery and Validation Of Body Fluid And Tissue Biomarkersmentioning

confidence: 99%

Forensic proteomics

Parker

McKiernan

Legg

et al. 2021

Forensic Science International: Genetics

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“…In addition to traditional (criminal justice) forensics, other fields also face the problem of characterizing a sample when little or nothing is known about its identity or origin, such as archaeology and cultural heritage. To cite just a few examples, proteomics has been used to characterize a victim's stomach contents upon autopsy, 60 identify trace evidence at a crime scene as vomit, 61 identify 3,500-year-old organic residue from tombs in northwestern China as a fermented dairy product, 62 and determine the host animal that was the source of a disease-vector tick's last blood meal. 63 All of the studies just mentioned used either database search or sequence-tag search to identify proteins.…”

Section: Forensics and Related Areasmentioning

confidence: 99%

Flying blind, or just flying under the radar? The underappreciated power of de novo methods of mass spectrometric peptide identification

2020

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Mass spectrometry‐based proteomics is a popular and powerful method for precise and highly multiplexed protein identification. The most common method of analyzing untargeted proteomics data is called database searching, where the database is simply a collection of protein sequences from the target organism, derived from genome sequencing. Experimental peptide tandem mass spectra are compared to simplified models of theoretical spectra calculated from the translated genomic sequences. However, in several interesting application areas, such as forensics, archaeology, venomics, and others, a genome sequence may not be available, or the correct genome sequence to use is not known. In these cases, de novo peptide identification can play an important role. De novo methods infer peptide sequence directly from the tandem mass spectrum without reference to a sequence database, usually using graph‐based or machine learning algorithms. In this review, we provide a basic overview of de novo peptide identification methods and applications, briefly covering de novo algorithms and tools, and focusing in more depth on recent applications from venomics, metaproteomics, forensics, and characterization of antibody drugs.

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“…Therefore, the identification and detailed characterization of ancient proteins from archeological and historic finds can reveal insights into past subsistence practices and physiopathological patterns, and can also provide new means to assess biological evolution and phylogenesis over the centuries . Modern MS-based proteomics has made the characterization of complex protein suites that occur in biological matrices at sub-picomolar scale a routine process and has impressively enlarged the variety of possible analytical approaches. Proteomics is a consolidated research tool in many fields of biological and biomedical research and can answer compelling questions in forensic science .…”

Section: Introductionmentioning

confidence: 99%

Proteomics and Integrated Techniques to Characterize Organic Residues in Funerary Findings from Italic Populations of the First Millennium BC

et al. 2022

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Multiple analytical techniques were combined to achieve a detailed characterization of organic residues in different typologies of funerary pottery, which were found at two separate archeological sites in the Campania Region (Italy) and both dated back to the first millennium BC. Gas chromatography−mass spectrometry (GC-MS) analysis of lipids provided inconclusive results. The attenuated total reflectance−Fourier transform infrared (ATR-FTIR) spectra of encrustation on two glazed bowls of the 3rd to 4th century BC were comparable to those of fresh bone, revealing the presence of hydroxyapatite and proteins, which were identified as bovine collagen chains by liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-MS/MS)-based proteomics. This finding confirmed that Italic populations used to inhume the dead along with votive meat offerings. Proteomics was decisive for identifying bovine milk in an unusually shaped amphora unearthed from a grave that belonged to a woman at the necropolis of the Greek colony in Cuma (7th century BC). Peptidomic analysis demonstrated that the genetic variant A 1 of β-casein was already present in the southern Mediterranean area at least 2500 years ago. Overall, these results depict an agropastoral system of Italic populations at the age of Magna Graecia based on a significant role of domesticated cattle.

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Mass spectrometry-based proteomics for the forensic identification of vomit traces

Cited by 12 publications

References 22 publications

Forensic proteomics

Forensic proteomics

Flying blind, or just flying under the radar? The underappreciated power of de novo methods of mass spectrometric peptide identification

Proteomics and Integrated Techniques to Characterize Organic Residues in Funerary Findings from Italic Populations of the First Millennium BC

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