Mass spectrometry of nucleic acids in molecular medicine

Ilina, Elena; Govorun, Vadim M.

doi:10.1134/s1068162009020010

Cited by 4 publications

(1 citation statement)

References 62 publications

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“…Within the framework of this review, we do not consider the varieties of genomics and transcriptomics; we only note the possible application of mass spectrometry in these fields. ESI-MS and MALD-IMS have the most significant opportunities for the study of DNA and RNA [ 11 ]. These methods can be used to determine the molecular weights of such macromolecules, their various complexes, and oligonucleotides, which is essential in studying their transformations.…”

Section: Mass Spectrometry In Omics Life Sciencesmentioning

confidence: 99%

Mass Spectrometry as a Crucial Analytical Basis for Omics Sciences

Заикин

Борисов

2021

J Anal Chem

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This review is devoted to the consideration of mass spectrometric platforms as applied to omics sciences. The most significant attention is paid to omics related to life sciences (genomics, proteomics, meta-bolomics, lipidomics, glycomics, plantomics, etc.). Mass spectrometric approaches to solving the problems of petroleomics, polymeromics, foodomics, humeomics, and exosomics, related to inorganic sciences, are also discussed. The review comparatively presents the advantages of various principles of separation and mass spectral techniques, complementary derivatization, used to obtain large arrays of various structural and quantitative information in the mentioned omics sciences.

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Section: Mass Spectrometry In Omics Life Sciencesmentioning

confidence: 99%

Mass Spectrometry as a Crucial Analytical Basis for Omics Sciences

Заикин

Борисов

2021

J Anal Chem

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Characterization of oligonucleotides with LNA-monomers for PCR detection of point mutations in mycobacteria tuberculosis genome

et al. 2012

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Point mutations associated with isoniazid resistance in Mycobacterium tuberculosis (MTB) have been analyzed in codon 315 of the katG gene by conventional polymerase chain reaction (PCR) using primers containing locked nucleic acid (LNA) modified nucleotides. Purity and structure of primers containing 5 LNA monomers of 17 nucleotides in length were characterized by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) and a 17-mer duplex formed by two complementary oligonucleotides was characterized by the method of thermal denaturation. The duplex containing five LNA monomers per each strand was characterized by a higher melting temperature than it was expected using extrapolation of theoretical calculation for nucleotide modification of one strand of the duplex. Detection of any of six possible mutations in katG codon 315 (i.e. discrimination between sensitive and resistant MTB) requires just one PCR employing a set of two primers with one LNA-modified primer; this is an important advantage of oligonucleotides containing LNA over unmodified nucleotides: employment of multiplex PCR would require up to 12 primers. Problems of control of oligonucleotide modification by LNA monomers are discussed.

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Molecular technologies used in detecting of sensitive and isoniazid-resistant Mycobacterium tuberculosis

Limanskaya

Limanskii

2011

Cytol. Genet.

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Mass spectrometry of nucleic acids in molecular medicine

Cited by 4 publications

References 62 publications

Mass Spectrometry as a Crucial Analytical Basis for Omics Sciences

Mass Spectrometry as a Crucial Analytical Basis for Omics Sciences

Characterization of oligonucleotides with LNA-monomers for PCR detection of point mutations in mycobacteria tuberculosis genome

Molecular technologies used in detecting of sensitive and isoniazid-resistant Mycobacterium tuberculosis

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