Mass Spectrometry

An Overview of Analytical Applications of Time of Flight-Mass Spectrometric (TOF-MS) Analyzers and an Inductively Coupled Plasma-TOF-MS Technique

“…For the GBC Optimass8000 instrument, the reported resolution ranges from approximately 500 for 6 Li to at least 2200 for 238 U. 155 The resolving power decreases towards low masses being, however, considerably higher than that observed for quadrupole systems.…”

“…Since its introduction in 1980, 5 ICP-MS has been widely used for the determination of low concentrations of the elements in a wide range of applications. [6][7][8][9][10][11][12][13][14][15] The technique offers excellent detection limits, a multi-element capability, selectivity, a possibility to evaluate isotopic ratios and a high sample throughput. Most ICP-MS spectrometers are equipped with scanning-mode quadrupole analyzers that can monitor only one mass/charge (m/z) at a given time period.…”

Capture and analysis of quantitative proteomic data

“…As the different control points in the regulation of gene expression are uncovered, from global mechanisms at the level of chromatin structure and transcriptional control, through to translational mechanisms including protein modification and turnover, the ability to quantify protein levels accurately will assume increasing importance. It is widely acknowledged that modern MS, coupled with online peptide separation methods and bioinformatics, can enable hundreds or thousands of peptides to be identified in a standard proteomics experiment [1][2][3][4][5][6] -the challenge is now to do this in a quantitative fashion, and translate these into protein levels. This will support the growing field of systems biology, where different concentrations of proteins determine various states of the sample being analysed and networks of interacting molecules are developed, ultimately to predict systems level phenomena.…”

Improvement in LC–MS calibration by addition of fragment signals

“…Its development over the last 15 years is related to the development of key ionisation techniques, such as electrospray ionisation (ESI) and atmospheric pressure chemical ionisation (APCI) [1]. Although ESI and APCI normally give little or no fragmentation, modern LC-MS instruments have methods for inducing fragmentation prior to mass separation.…”