Mass transfer in a spray column during two-phase extraction of horseradish peroxidase

Srinivas, N. D.; Narayan, A. V.; Raghavarao, K.S.M.S.

doi:10.1016/s0032-9592(02)00097-3

Cited by 28 publications

(15 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These results are within the range reported for the operation of columns with PEG/salt [29][30][31]. As previously observed for such systems, a fast hold-up increase with rising flow rate was also observed [32,33].…”

Section: Hydrodynamicssupporting

confidence: 90%

Multi‐Stage Aqueous Two‐Phase Extraction for the Purification of Monoclonal Antibodies

et al. 2014

View full text Add to dashboard Cite

Using monoclonal antibodies in a cell culture harvest as an example for complex biomolecules, a mini-plant-scale aqueous two-phase purification process was studied. The results of this study indicate that antibodies can be concentrated and purified in a single extraction step employing a small phase ratio. Following the extraction step, a multi-stage wash-extraction for further purification was investigated. Starting at a test tube-scale cross-current wash, the process was advanced towards a continuous counter-current mixer-settler and column wash process. It was shown that a test tube cross-current extraction operation can predict the multi-stage purity reasonably well. The hydrodynamic process performance for the multi-stage wash column was evaluated and related to the separation performance.

show abstract

Section: Hydrodynamicssupporting

confidence: 90%

Multi‐Stage Aqueous Two‐Phase Extraction for the Purification of Monoclonal Antibodies

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The definitions of process parameters evaluated for the extraction by ATPS in continuous mode, and the related equations were already described by several authors [7,10,11,20].…”

Section: Definition Of Process Parametersmentioning

confidence: 99%

Extraction of Ascorbate Oxidase from Cucurbita maxima by Continuous Process in Perforated Rotating Disc Contactor Using Aqueous Two-Phase Systems

Porto

Marques

Porto

et al. 2009

Appl Biochem Biotechnol

View full text Add to dashboard Cite

The ascorbate oxidase is the enzyme used to determine the content of ascorbic acid in the pharmaceutical and food industries and clinics analyses. The techniques currently used for the purification of this enzyme raise its production cost. Thus, the development of alternative processes and with the potential to reduce costs is interesting. The application of aqueous two-phase system is proposed as an alternative to purification because it enables good separation of biomolecules. The objective of this study was to determine the conditions to continuously pre-purify the enzyme ascorbate oxidase by an aqueous two-phase system (PEG/citrate) using rotating column provided with perforated discs. Under the best conditions (20,000 g/mol PEG molar mass, 10% PEG concentration, and 25% citrate concentration), the system showed satisfactory results (partition coefficient, 3.35; separation efficiency, 54.98%; and purification factor, 1.46) and proved suitable for the pre-purification of ascorbate oxidase in continuous process.

show abstract

“…Furthermore, up to now, most of that processes were performed in agitated vessels followed by centrifugation or very long gravity phase separation (>14 h) due to relative stability of the dispersion produced in the agitated vessel (Hart et al, 1994;Kepka et al, 2003;Kroner et al, 1982;Selber et al, 2004). This equipment assembly (agitated vessel + centrifuge) provides only one theoretical stage and is expensive at large scale (Srinivas et al, 2002). Thus, as in many ATPS more than one theoretical stage is necessary to achieve the desired yield and purity, multi-stage equipment, such as mixer-settler battery or column extractors, may be employed as alternative extraction equipment as they are known to provide more than one theoretical stage and do not require centrifugation to achieve faster phase separation (Srinivas et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…This equipment assembly (agitated vessel + centrifuge) provides only one theoretical stage and is expensive at large scale (Srinivas et al, 2002). Thus, as in many ATPS more than one theoretical stage is necessary to achieve the desired yield and purity, multi-stage equipment, such as mixer-settler battery or column extractors, may be employed as alternative extraction equipment as they are known to provide more than one theoretical stage and do not require centrifugation to achieve faster phase separation (Srinivas et al, 2002). Studies regarding the multi-stage equilibrium should then be performed in order to evaluate the possibility of using typical multi-stage liquid-liquid extraction equipment.…”

Section: Introductionmentioning

confidence: 99%

Application of aqueous two-phase systems to antibody purification: A multi-stage approach

Rosa

Azevedo

Sommerfeld

et al. 2009

Journal of Biotechnology

105

102

View full text Add to dashboard Cite

Mass transfer in a spray column during two-phase extraction of horseradish peroxidase

Cited by 28 publications

References 2 publications

Multi‐Stage Aqueous Two‐Phase Extraction for the Purification of Monoclonal Antibodies

Multi‐Stage Aqueous Two‐Phase Extraction for the Purification of Monoclonal Antibodies

Extraction of Ascorbate Oxidase from Cucurbita maxima by Continuous Process in Perforated Rotating Disc Contactor Using Aqueous Two-Phase Systems

Application of aqueous two-phase systems to antibody purification: A multi-stage approach

Contact Info

Product

Resources

About