Mass transfer in aqueous two-phases system packed column

Continuous aqueous two-phase extraction of human antibodies using a packed column

“…Column extractors used for the conventional organic-aqueous liquid-liquid extraction (LLE) operations in the chemical industry (e.g. spray and packed column) can be adapted for continuous ATPE of proteins [12] as an alternative to the typically used batch equipment assembly. In fact, they are known to provide more than one theoretical stage improving process efficiency, and do not require the use of a centrifuge to achieve faster phase separation by gravity settling leading to a more cost-effective process [12,13].…”

Application of the response surface methodology for optimization of whey protein partitioning in PEG/phosphate aqueous two-phase system

“…The high water content of the system ensures molecular stability of proteins throughout the extraction process [19]. Moreover, extraction using ATPS is suitable for continuous purification processes using equipment traditionally applied in conventional liquid-liquid extraction [20].…”

Extraction of Ascorbate Oxidase from Cucurbita maxima by Continuous Process in Perforated Rotating Disc Contactor Using Aqueous Two-Phase Systems

“…Liquid-liquid extraction equipments are widely used in biotechnology process for extraction of proteins [8,9], enzymes [8,[10][11][12], toxin [7], etc. The rotating disc contactor, consisting of a vertical shell equipped with a series of rotor discs and stators, can be used for this process [13].…”