Mast Cell Development and Biostresses: Different Stromal Responses in Bone Marrow and Spleen after Treatment of Myeloablater, 5-Fluorouracil, and Inflammatory Stressor, Lipopolysaccharide

Hokari, Takeo; Tsuboi, Isao; Harada, Toshiro; Oshima, Hideki; Hirabayashi, Yoko; Kanno, Jun; Inoue, Tohru; Aizawa, Shin

doi:10.1248/bpb.34.1533

Cited by 6 publications

(13 citation statements)

References 21 publications

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“…25,26) In contrast, an increase in the number of CFU-preB cells was not observed. It is reported that increased CFU-preB number in the spleen was observed in C57 BL/6 mice immunized with acetyl-chicken γ globulin, of which phenomenon was attributed to that the down-regulation of SDF-1 in the BM facilitated the release of CFU-preB cells from the BM to the spleen.…”

Section: Discussionmentioning

confidence: 82%

“…These results are compatible with previous reports. 5,25,31) Flow cytometry analysis showed that the proportion of immature myeloid cells in the BM was increased in parallel with the increase in CFU-GM cells 3 d after LPS treatment, which indicates that myelopoiesis in the BM was activated soon after LPS treatment (Figs. 1B, 3).…”

Section: Discussionmentioning

confidence: 93%

“…25,26) A control group of mice was injected with the same volume of pyrogen-free saline (i.e., 0.2 mL per mouse). Three mice from each treatment group were then evaluated 1, 3, and 6 h and 1, 2, 3, 5, and 7 d after treatment with LPS or saline.…”

Section: Methodsmentioning

confidence: 99%

“…HU (Sigma-Aldrich) was dissolved in IMDM and added to one tube at a final concentration of 6×10 −3 M. 25,26,28) IMDM alone was added to the control. Both tubes were incubated for 1 h at 37°C.…”

Section: Methodsmentioning

confidence: 99%

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Lipopolysaccharide Reciprocally Alters the Stromal Cell-Regulated Positive and Negative Balance between Myelopoiesis and B Lymphopoiesis in C57BL/6 Mice

Taki

Tsuboi

Harada

et al. 2014

Biological & Pharmaceutical Bulletin

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Hematopoiesis in the bone marrow (BM) and spleen is controlled by stromal cells. Inflammation promotes myelopoiesis and simultaneously suppresses B lymphopoiesis. However, the role of the reciprocal regulation of myelopoiesis and B lymphopoiesis by stromal cells during inflammation is not fully understood. We investigated inflammation-induced alteration of hematopoietic regulation in lipopolysaccharide (LPS)-treated mice. C57BL/6 female mice were intravenously injected with a single, 5-µg dose of LPS, which induced a rapid decrease in the number of granulocyte-macrophage progenitors (colony-forming unit granulocyte-macrophage; CFU-GM) and B cell progenitors (CFU-preB) in BM. The CFU-GM count rapidly recovered, whereas the recovery of CFU-preB was delayed. LPS induced a marked increase in the number of CFU-GM but not in the number of CFU-preB in spleen. After LPS treatment, gene expression levels of positive regulators of myelopoiesis such as granulocyte colony-stimulating factor (G-CSF), interleukin (IL)-6, and granulocyte-macrophage colony-stimulating factor (GM-CSF) in BM and spleen were markedly upregulated whereas levels of positive regulators for B lymphopoiesis such as stromal cell-derived factor (SDF)-1, stem cell factor (SCF), and IL-7 remained unchanged. Meanwhile, the negative regulator of B lymphopoiesis tumor necrosis factor (TNF)-α was markedly up-regulated. The number of CFU-GM in S-phase in BM increased after LPS treatment, whereas the number of CFU-preB in S-phase decreased. These results suggest that LPS-activated stromal cells induce positive-dominant regulation of myelopoiesis and negative-dominant regulation of B lymphopoiesis, which facilitates emergency myelopoiesis during inflammation by suppressing B lymphopoiesis, thereby contributing to the host defense against infection.

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Lipopolysaccharide Reciprocally Alters the Stromal Cell-Regulated Positive and Negative Balance between Myelopoiesis and B Lymphopoiesis in C57BL/6 Mice

Taki

Tsuboi

Harada

et al. 2014

Biological & Pharmaceutical Bulletin

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“…However, all the above are proven activities on isolated single type of cells [14][15][16][17][18]. In vivo where cell-cell interaction is evident, LPS administration was found herein and by others to increase TNF-α, IL-12, and IL-10 and decrease TGF-β production in mice spleen indicating the favor of one signal pathway over the other in case of LPS proinflammatory induction and the suppression of some regulator pathways (Table 1) [19,20].…”

Section: Discussionmentioning

confidence: 71%

Anti-inflammatory aminoacetylenic isoindoline-1,3-dione derivatives modulate cytokines production from different spleen cell populations

Matalka

Alfarhoud

Qinna

et al. 2012

International Immunopharmacology

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Use of fluorescence microspectral method for studying bone marrow EGFP+ cell transplantation in 5-fluorouracil-treated mice

et al. 2014

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In this paper the experimental results of bone marrow transplantation from C57BL/6-Tg(ACTB EGFP)1Osb/J transgenic mice into C57BL/6 mice subjected to 5 fluorouracil treatment are represented. It has been shown that EGFP + cell engraftment in bone marrow, spleen and thymus of host mice after 5 Fu treatment significantly increased. More long term engraftment was recorded after transplantation between closely related donors and 5 fluorouracil treatment hosts. We have also obtained data on differences in the dynamics of EGFP + cell engraftment in host investigated organs. To assess the effect of the donor's bone marrow cells on the host immune system, functional activity of the synthetic apparatus (synthetic activity) of cells in bone marrow, spleen, thymus and blood have been investigated with fluorescence microspectral method. The results obtained allow of improving techniques for bone marrow transplantation without host irradiation in order to minimize the adverse effects.

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Mast Cell Development and Biostresses: Different Stromal Responses in Bone Marrow and Spleen after Treatment of Myeloablater, 5-Fluorouracil, and Inflammatory Stressor, Lipopolysaccharide

Cited by 6 publications

References 21 publications

Lipopolysaccharide Reciprocally Alters the Stromal Cell-Regulated Positive and Negative Balance between Myelopoiesis and B Lymphopoiesis in C57BL/6 Mice

Lipopolysaccharide Reciprocally Alters the Stromal Cell-Regulated Positive and Negative Balance between Myelopoiesis and B Lymphopoiesis in C57BL/6 Mice

Anti-inflammatory aminoacetylenic isoindoline-1,3-dione derivatives modulate cytokines production from different spleen cell populations

Use of fluorescence microspectral method for studying bone marrow EGFP+ cell transplantation in 5-fluorouracil-treated mice

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