Mast Cell-Nerve Cell Interaction at Acupoint: Modeling Mechanotransduction Pathway Induced by Acupuncture

Yao, Wei; Yang, Hongwei; Yin, Na; Ding, Guanghong

doi:10.7150/ijbs.8631

Cited by 51 publications

(32 citation statements)

References 42 publications

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“…Mast cell-neuron interaction contributes to the modulation of signal transmission pathways. A model of mast cellneuron interaction at acupoint has been proposed to study Ca 2+ signaling and ATP release in mast cells and neurons and applied to investigate the coupled response of mast cells and the nerve system to mechanical stimuli (Yao et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Local cutaneous nerve terminal and mast cell responses to manual acupuncture in acupoint LI4 area of the rats

Wei

et al. 2015

Journal of Chemical Neuroanatomy

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Previous studies have shown that the effects of manual acupuncture (MA) are contributed by collagen fibers and mast cells in local acupoints, at which acupuncture stimulation causes various afferent fiber groups to be excited. However what happens in local nerve fibers and mast cells after MA remains unclear. The aim of this study was to examine the response of cutaneous nerve fibers and mast cells to MA stimulation in acupoint Hegu (LI4). The contralateral LI4 of the same rat was used as a non-stimulated control. Immnohistochemistry analysis were carried out to observe the expression of histamine (HA), serotonin (5-HT) and nociceptive neuropeptides, calcitonin gene-related peptide (CGRP) and substance P (SP), in the LI4 area. Mast cells were labeled with anti-mast cell tryptase antibody and simultaneously with HA or 5-HT primary antibodies to observe their co-expression. Our results showed that SP and CGRP were expressed more highly on the cutaneous nerve fibers of LI4 after MA stimulation than that of the control. Mast cells aggregated in close proximity to the blood vessels in intra-epidermis and dermis and some of them with degranulation in the lower dermis and subcutaneous tissue of LI4. Both mast cells and their granules appeared with HA (+) and 5-HT (+) expression at stimulated L14 sites, while a few intact mast cells with a little expression of 5-HT and HA were distributed in areas of non-stimulated L14. The results indicated that local cutaneous nerve terminals and mast cells responded to MA with higher expression of SP and CGRP in nerve fibers, as well as with aggregation and degranulation of mast cells with HA and 5-HT granules at acupoint LI4. These neuroactive substances may convey signals to certain pathways that contribute to the effects of acupuncture.

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Section: Introductionmentioning

confidence: 99%

Local cutaneous nerve terminal and mast cell responses to manual acupuncture in acupoint LI4 area of the rats

Wei

et al. 2015

Journal of Chemical Neuroanatomy

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Section: The Effect Of Dry Needling Mechanotransduction On Mast Cellsmentioning

confidence: 99%

“…Some factors initially released during degranulation such as cytokines, serotonin and SP result in discomfort and may add to the perception of de qi [80,102]. However, given that MCs are primarily located in loose connective tissue, the cells effectively connect sensory afferents to blood and lymphatic vessels and therefore play a role in vasodilation by releasing histamine, heparin, leukotrienes and NO [80,102,105]. While MC degranulation also directly results in ATP release [102,105], histamine further stimulates ATP release from subcutaneous fibroblasts, amplifying P2X/P2Y and adenosine mediated pain reduction [83].…”

Section: The Effect Of Dry Needling Mechanotransduction On Mast Cellsmentioning

confidence: 99%

“…A number of studies have linked the physical stimulation of needles with the activation of stretchsensitive chloride channels on MCs, resulting in degranulation [83,[102][103][104]. Some factors initially released during degranulation such as cytokines, serotonin and SP result in discomfort and may add to the perception of de qi [80,102]. However, given that MCs are primarily located in loose connective tissue, the cells effectively connect sensory afferents to blood and lymphatic vessels and therefore play a role in vasodilation by releasing histamine, heparin, leukotrienes and NO [80,102,105].…”

Section: The Effect Of Dry Needling Mechanotransduction On Mast Cellsmentioning

confidence: 99%

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Peripheral and Spinal Mechanisms of Pain and Dry Needling Mediated Analgesia: A Clinical Resource Guide for Health Care Professionals

Butts¹,

Dunning²

2016

Int J Phys Med Rehabil

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There are a number of biochemical, biomechanical, endocrinological and neurovascular mechanisms underpinning the anti-nociceptive and anti-inflammatory effects of dry needling (DN). While myofascial trigger points likely play a role in peripheral pain, a diagnostic tool for localizing them has not been validated, and DN studies that have targeted trigger points to elicit localized twitch responses have reported mixed results. Therefore, the mechanism responsible for DN-mediated analgesia may be more complicated. DN activates opioid-based pain reduction, mediated by endogenous cannabinoids and the sympathetic nervous system, and non-opioid pain relief via serotonin and norepinephrine from the brain stem. DN also triggers the hypothalamic-pituitary-adrenal axis centrally and the corticotropin releasing hormone-proopiomelanocortin-corticosteroid axis locally to inhibit cox-2, reducing inflammatory cytokines. Recent studies demonstrate that DN combined with mechanical and/or electrical stimulation may reverse PKC-mediated peripheral hyperalgesic priming by normalizing nociceptive channels, to include TRPV, ASIC, TTX and P2X/Y. Electrical DN (EDN) stimulates immune cells, fibroblasts and keratinocytes to release CGRP and substance-P, altering the stimulation of TTX receptors to reverse hyperalgesia. It also encourages the supraoptic nucleus to release oxytocin to quiet ASIC receptors peripherally and stimulate opioid interneurons spinally. Moreover, EDN inhibits ERK1/2 kinase pathways of inflammation in the spinal cord and stimulates Aδ fibers and N/OFQ to reverse C-fiber mediated central changes. Mechanotransduction of fibroblasts and peripheral nerves via TRPV1 and P2X/Y-mediated intracellular Ca 2+ wave propagation and subsequent activation of the nucleus accumbens inhibits spinal pain transmission via glycinergic and opioidergic interneurons. The increased ATP is metabolized to adenosine, which activates P1 purinergic receptors, events considered key to DN analgesia and rho kinase-based tissue remodeling. Mechanotransduction-mediated release of histamine further explains analgesia secondary to needling points distal to pain. DN-mediated analgesia is dependent on a number of synergistic physiologic events involving biochemical and mechanical processes in neural, connective and muscle tissue.

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“…We have set up a mathematical model to explore signaling pathways in mast cells and clarified mast cells' participating in acupuncture effect [15]. Furthermore, we construct a mathematical model for describing mast cell and nerve cell interaction modulated by acupuncture [16].…”

Section: Introductionmentioning

confidence: 99%

A Mathematical Model for the Instigation and Transmission of Biological and Neural Signals in Response to Acupuncture

Yao

Yin

Yang

et al. 2015

Commun. Comput. Phys.

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Acupuncture has been in clinical practice in China for thousands of years and its analgesia effect is worldwide accepted. However, the mechanism of acupuncture effect is not well understood. The study focus on signaling pathways induced by acupuncture, analyzes the cooperative action of the acupoints’ structure and the associated chemical mediators during acupuncture, establishes a mathematical model clarifying the roadmap of electroneurographic signal startup and transmission mechanism induced by acupuncture, quantitatively analyzing the response in acupoints to acupuncture. These work contribute to reveal the activation and transmission mechanism of neural signals induced by acupuncture from systems biology perspective, lay the foundation for the integration of acupuncture theory and modern science and further guide the clinical treatment and experimental research of acupuncture.

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Mast Cell-Nerve Cell Interaction at Acupoint: Modeling Mechanotransduction Pathway Induced by Acupuncture

Cited by 51 publications

References 42 publications

Local cutaneous nerve terminal and mast cell responses to manual acupuncture in acupoint LI4 area of the rats

Local cutaneous nerve terminal and mast cell responses to manual acupuncture in acupoint LI4 area of the rats

Peripheral and Spinal Mechanisms of Pain and Dry Needling Mediated Analgesia: A Clinical Resource Guide for Health Care Professionals

A Mathematical Model for the Instigation and Transmission of Biological and Neural Signals in Response to Acupuncture

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