Mast Cells in the Auditory Periphery of Rodents

Szczepek, Agnieszka J.; Dudnik, Tatyana; Karayay, Betül; Sergeeva, V. E.; Smorodchenko, Alina

doi:10.3390/brainsci10100697

Cited by 8 publications

(7 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The spiral prominence showed rare cells expressing CD117 (KIT). Recently, mast cells were localized in the mouse and rat modiolus, spiral ligament, and stria vascularis using antibodies against c-Kit/CD117 ( 78 ). Their role in cochlear development, homeostasis, and pathology was discussed.…”

Section: Discussionmentioning

confidence: 99%

Immuno-surveillance and protection of the human cochlea

Liu,

Li,

Kämpfe Nordström

et al. 2024

Front. Neurol.

View full text Add to dashboard Cite

BackgroundDespite its location near infection-prone areas, the human inner ear demonstrates remarkable resilience. This suggests that there are inherent instruments deterring the invasion and spread of pathogens into the inner ear. Here, we combined high-resolution light microscopy, super-resolution immunohistochemistry (SR-SIM) and synchrotron phase contrast imaging (SR-PCI) to identify the protection and barrier systems in the various parts of the human inner ear, focusing on the lateral wall, spiral ganglion, and endolymphatic sac.Materials and methodsLight microscopy was conducted on mid-modiolar, semi-thin sections, after direct glutaraldehyde/osmium tetroxide fixation. The tonotopic locations were estimated using SR-PCI and 3D reconstruction in cadaveric specimens. The sections were analyzed for leucocyte and macrophage activity, and the results were correlated with immunohistochemistry using confocal microscopy and SR-SIM.ResultsLight microscopy revealed unprecedented preservation of cell anatomy and several macrophage-like cells that were localized in the cochlea. Immunohistochemistry demonstrated IBA1 cells frequently co-expressing MHC II in the spiral ganglion, nerve fibers, lateral wall, spiral limbus, and tympanic covering layer at all cochlear turns as well as in the endolymphatic sac. RNAscope assays revealed extensive expression of fractalkine gene transcripts in type I spiral ganglion cells. CD4 and CD8 cells occasionally surrounded blood vessels in the modiolus and lateral wall. TMEM119 and P2Y12 were not expressed, indicating that the cells labeled with IBA1 were not microglia. The round window niche, compact basilar membrane, and secondary spiral lamina may form protective shields in the cochlear base.DiscussionThe results suggest that the human cochlea is surveilled by dwelling and circulating immune cells. Resident and blood-borne macrophages may initiate protective immune responses via chemokine signaling in the lateral wall, spiral lamina, and spiral ganglion at different frequency locations. Synchrotron imaging revealed intriguing protective barriers in the base of the cochlea. The role of the endolymphatic sac in human inner ear innate and adaptive immunity is discussed.

show abstract

Section: Discussionmentioning

confidence: 99%

Immuno-surveillance and protection of the human cochlea

Liu,

Li,

Kämpfe Nordström

et al. 2024

Front. Neurol.

View full text Add to dashboard Cite

show abstract

“…A few research teams have identified the expression of histamine receptors in the inner ear of mammals [38][39][40][41]. Additionally, in 2020, our group found mast cells in the inner ear of both rats and mice [42]. Mast cells are the major source of histamine in the body, along with basophils, gastric parietal cells, and the central nervous system [43].…”

Section: Introductionmentioning

confidence: 89%

Histamine and Its Receptors in the Mammalian Inner Ear: A Scoping Review

2023

Self Cite

View full text Add to dashboard Cite

Background: Histamine is a widely distributed biogenic amine with multiple biological functions mediated by specific receptors that determine the local effects of histamine. This review aims to summarize the published findings on the expression and functional roles of histamine receptors in the inner ear and to identify potential research hotspots and gaps. Methods: A search of the electronic databases PubMed, Web of Science, and OVID EMBASE was performed using the keywords histamine, cochlea*, and inner ear. Of the 181 studies identified, 18 eligible publications were included in the full-text analysis. Results: All four types of histamine receptors were identified in the mammalian inner ear. The functional studies of histamine in the inner ear were mainly in vitro. Clinical evidence suggests that histamine and its receptors may play a role in Ménière’s disease, but the exact mechanism is not fully understood. The effects of histamine on hearing development remain unclear. Conclusions: Existing studies have successfully determined the expression of all four histamine receptors in the mammalian inner ear. However, further functional studies are needed to explore the potential of histamine receptors as targets for the treatment of hearing and balance disorders.

show abstract

“…Postnatal mouse brain and adult mouse spleen tissues were harvested and used as positive and negative controls, respectively. The Western blot protocol was previously described [ 51 ]. Briefly, the tissue lysates were prepared by placing and vortexing the explants in 80 µL RIPA buffer (Cell Signaling Technology, Danvers, MA, USA) and then centrifuging them at 14,000 RPM at +4 °C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Identification and Characterization of TMEM119-Positive Cells in the Postnatal and Adult Murine Cochlea

2023

Self Cite

View full text Add to dashboard Cite

Transmembrane protein 119 (TMEM119) is expressed in a subset of resident macrophage cells of the brain and was proposed as a marker for native brain microglia. The presence of cells expressing TMEM119 in the cochlea has not yet been described. Thus, the present study aimed to characterize the TMEM119-expressing cells of the postnatal and adult cochlea, the latter also after noise exposure. Immunofluorescent staining of cochlear cryosections detected TMEM119 protein in the spiral limbus fibrocytes and the developing stria vascularis at postnatal Day 3. Applying the macrophage marker Iba1 revealed that TMEM119 is not a marker of cochlear macrophages or a subset of them. In the adult murine cochlea, TMEM119 expression was detected in the basal cells of the stria vascularis and the dark mesenchymal cells of the supralimbal zone. Exposure to noise trauma was not associated with a qualitative change in the types or distributions of the TMEM119-expressing cells of the adult cochlea. Western blot analysis indicated a similar TMEM119 protein expression level in the postnatal cochlea and brain tissues. The findings do not support using TMEM119 as a specific microglial or macrophage marker in the cochlea. The precise role of TMEM119 in the cochlea remains to be investigated through functional experiments. TMEM119 expression in the basal cells of the stria vascularis implies a possible role in the gap junction system of the blood–labyrinth barrier and merits further research.

show abstract

Mast Cells in the Auditory Periphery of Rodents

Cited by 8 publications

References 54 publications

Immuno-surveillance and protection of the human cochlea

Immuno-surveillance and protection of the human cochlea

Histamine and Its Receptors in the Mammalian Inner Ear: A Scoping Review

Identification and Characterization of TMEM119-Positive Cells in the Postnatal and Adult Murine Cochlea

Contact Info

Product

Resources

About