Mast Cells Interact with Endothelial Cells to Accelerate In Vitro Angiogenesis

Souza, Devandir Antonio de; Mazucato, Vivian Marino; Santana, Ana Carolina; Oliver, Constance; Jamur, Maria Célia

doi:10.20944/preprints201710.0190.v1

Cited by 8 publications

(2 citation statements)

References 50 publications

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“…MC have been shown to produce IL-1β 32 and to interact with several other cell types including macrophages, T cells, and endothelial cells. [33][34][35][36][37] An in vitro study showed that β-tryptase stimulates the production of IL-1β in endothelial cells from human umbilical veins in vitro. 20 Further, MC activation induces IL1B expression in CD68positive synovial macrophages in cultured SM cells derived from RA patients.…”

Section: Discussionmentioning

confidence: 99%

<p>Increase in Tryptase and Its Role in the Synovial Membrane of Overweight and Obese Patients with Osteoarthritis of the Knee</p>

Takata¹,

Uchida²,

Mukai³

et al. 2020

DMSO

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The mechanisms governing evidence that obesity is a risk factor for osteoarthritis (OA) are not well understood. We previously reported an increase in mast cell (MC) marker expression in the osteoarthritic synovial membrane (SM) of patients with obesity. We hypothesized that an enzyme produced by MC, β-tryptase, may be increased in the SM of obese patients with knee OA and contribute to synovial inflammation. This study investigated the expression of the β-tryptase encoding gene, TPSB2, in the SM of obese patients with knee OA and β-tryptase-mediated regulation of IL-1β in synovial cells. Patients and Methods: A total of 216 patients radiographically diagnosed with knee OA were grouped according to the World Health Organization's body mass index classifications: normal weight (NW; <25 kg/m 2), overweight (OW; 25-29.99 kg/m 2) and obese (OB; ≥30 kg/ m 2). Quantitative polymerase chain reaction was conducted to examine TPSB2 expression in the SM among the three groups. We also examined TPSB2 and IL1B expression in MC-rich (CD3-CD14-CD19-CD90-) and MC-poor (CD3+, CD14+, CD19+, or CD90+) fractions freshly isolated from synovial tissue. Further, the effect of β-tryptase on IL1B expression was investigated in cultured CD14-positive (macrophage-rich fraction) and CD14-negative (fibroblast-rich fraction) cells. Results: There was significantly elevated TPSB2 expression in the OW and OB groups compared to the NW group. The MC-rich fraction had significantly higher levels of TPSB2, CD117 and CD203c than the MC-poor fraction. Recombinant human β-tryptase stimulated IL1B expression in both the synovial fibroblast and macrophage fractions. Conclusion: Obese patients with knee OA showed elevated TPSB2 expression in the SM. Tryptase may play a role in synovial inflammation in obese patients with OA.

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Section: Discussionmentioning

confidence: 99%

<p>Increase in Tryptase and Its Role in the Synovial Membrane of Overweight and Obese Patients with Osteoarthritis of the Knee</p>

Takata¹,

Uchida²,

Mukai³

et al. 2020

DMSO

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“…On the other hand, gap junctions can be engaged in effects of MCs proliferation and phenotype of fibroblasts observed in vitro [ 120 , 121 ]. There is also a report regarding the formation of gap junctions between mouse mastocytoma and endothelial cell lines associated with increased angiogenesis activity of endothelial cells [ 122 ].…”

Section: Mast Cells and Intercellular Junctionsmentioning

confidence: 99%

The Art of Mast Cell Adhesion

Pastwińska

Żelechowska

Walczak‐Drzewiecka

et al. 2020

Cells

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Cell adhesion is one of the basic phenomena occurring in a living organism, affecting many other processes such as proliferation, differentiation, migration, or cell viability. Mast cells (MCs) are important elements involved in defending the host against various pathogens and regulating inflammatory processes. Due to numerous mediators, they are contributing to the modulation of many basic cellular processes in a variety of cells, including the expression and functioning of different adhesive molecules. They also express themselves many adhesive proteins, including ICAM-1, ICAM-3, VCAM-1, integrins, L-selectin, E-cadherin, and N-cadherin. These molecules enable MCs to interact with other cells and components of the extracellular matrix (ECM), creating structures such as adherens junctions and focal adhesion sites, and triggering a signaling cascade. A thorough understanding of these cellular mechanisms can create a better understanding of MC biology and reveal new goals for MC targeted therapy. This review will focus on the current knowledge of adhesion mechanisms with the involvement of MCs. It also provides insight into the influence of MCs or MC-derived mediators on the adhesion molecule expression in different cells.

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Metachromatic, tryptase- and ghrelin-positive mast cells in the blood vessels of rat’s lung

Ivanova

2023

BJVM

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Mast cells and the mediators they release play a major role in the pathogenesis of lung diseases. They regulate the function of the smooth muscle layer of the pulmonary airways and the same layer of the blood vessels. There are no data about the distribution of metachromatic and tryptase-positive mast cells in the blood vessels’ wall as well as on the content of ghrelin in mast cell granules in rat’s lung. The aim of this research was to present an analysis of the number and localisation of tryptase-positive, ghrelin-positive and metachromatic mast cells in the blood vessels (arteries, capillaries, venules and veins) in rats of different age. Six male Wistar rats were used for each age group - 20 days, 3 months and 1 year. Tissue slices for histochemical examination were taken from the caudal lobe of the left lung and stained with toluidine blue. Immunohistochemical reactions were then performed to indicate the expression of tryptase and ghrelin, allowing comparing the presence of tryptase-, ghrelin-positive and metachromatic mast cells in the wall of the blood vessels of different diameters. The light microscopical study showed that in all types of blood vessels from the three age groups, the number of ghrelin positive cells was the largest, followed by tryptase-positive and metachromatic mast cells. The observed differences in the distribution of these cells are important for maintaining the lung homeostasis and can be used as reference values in experimental studies in order to obtain accurate results.

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Mast Cells Interact with Endothelial Cells to Accelerate In Vitro Angiogenesis

Cited by 8 publications

References 50 publications

<p>Increase in Tryptase and Its Role in the Synovial Membrane of Overweight and Obese Patients with Osteoarthritis of the Knee</p>

<p>Increase in Tryptase and Its Role in the Synovial Membrane of Overweight and Obese Patients with Osteoarthritis of the Knee</p>

The Art of Mast Cell Adhesion

Metachromatic, tryptase- and ghrelin-positive mast cells in the blood vessels of rat’s lung

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