MASTR Pouch: Palm-size lab for point-of-care detection of Mpox using recombinase polymerase amplification and CRISPR technology

Jia, Wei Hua; Wang, Wanjun; Yu, Qiong; Zhang, Mingrui; Xue, Fengyu; Fan, Beibei; Zhang, Tong; Gao, Yanpeng; Li, Juan; Pang, Bo

doi:10.1016/j.snb.2023.133950

Cited by 10 publications

(2 citation statements)

References 22 publications

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“…23,24 Previously, a few reports have demonstrated CRISPR-based detection of mpox virus. This study differs from the previous reports in two aspects; single copy detection for two genes/targets was not reported 20,21,25,26 and few reports have considered only a single locus for detection. 19,27 Our CRISPR-Cas12a-based assay is able to specifically detect mpox virus with an LOD of 1 copy/µL for both the targets.…”

Section: Conclusion and Discussioncontrasting

confidence: 81%

CRISPR‐Cas12a assisted specific detection of mpox virus

Singh

Misra

Bindal

et al. 2023

Journal of Medical Virology

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Mpox virus, a member of genus Orthopoxvirus, causes rash and flu‐like symptoms in humans. In the recent global outbreak, it was reported from several geographical areas that have not historically reported mpox. Point of care, sensitive and specific mpox diagnostic assays are critical in checking the spread of the disease. We have developed a clustered regularly interspaced short palindromic repeats associated Cas12a nuclease‐based assay for detecting mpox virus. Mpox specific conserved sequences were identified in polA (E9L) gene which differ by a single nucleotide polymorphism (SNP) from all the viruses present in the genus Orthopoxvirus. This SNP was exploited in our assay to specifically distinguish mpox virus from other related orthopox viruses with a limit of detection of 1 copy/μl in 30 min. The assay exhibits a sensitive and specific detection of mpox virus which can prove to be of practical value for its surveillance in areas infected with multiple orthopox viruses, especially in hotspots of mpox virus infections.

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