Maternal Embryo Effect Arrest 31 (MEE31) is a moonlighting protein involved in GDP-D-mannose biosynthesis and KAT1 potassium channel regulation

González-García, Adrián; Kanli, Maria; Wisowski, Natalia; Montoliu-Silvestre, Eva; Locascio, Antonella; Sifres, Alicia; Gómez, Marcos; Ramos, José; Porcel, Rosa; Andrés-Colás, Nuria; Mulet, José Miguel; Yenush, Lynne

doi:10.1016/j.plantsci.2023.111897

Cited by 2 publications

(1 citation statement)

References 73 publications

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“…Arabidopsis thaliana seeds used in this manuscript include the previously described aba2/gin1-3 ( aba2 ) 36 , pifq 69 , pPIF3::YFP:PIF3 (YFP- PIF3) 42 , p35S::PIF4-HA (PIF4-HA) 70 , pif1-1 71 , pif3-3 72 , pif4-2 73 , pif5-3 74 , PIF3-OX 75 , PIF5-OX 74 , phyAB 76 , prr5-1 77 , kat1-1 and kat1-2 53 , and 35S:KAT1-YFP ( KAT1OX ) 78 in Col-0 ecotype. The expression of KAT1 in the KAT1OX line is approximately 12-fold higher compared to Col-0 at ZT0 in SD (Supplementary Fig.…”

Section: Methodsmentioning

confidence: 99%

PIF transcriptional regulators are required for rhythmic stomatal movements

Rovira,

Veciana,

Basté-Miquel

et al. 2024

Nat Commun

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Stomata govern the gaseous exchange between the leaf and the external atmosphere, and their function is essential for photosynthesis and the global carbon and oxygen cycles. Rhythmic stomata movements in daily dark/light cycles prevent water loss at night and allow CO2 uptake during the day. How the actors involved are transcriptionally regulated and how this might contribute to rhythmicity is largely unknown. Here, we show that morning stomata opening depends on the previous night period. The transcription factors PHYTOCHROME-INTERACTING FACTORS (PIFs) accumulate at the end of the night and directly induce the guard cell-specific K+ channel KAT1. Remarkably, PIFs and KAT1 are required for blue light-induced stomata opening. Together, our data establish a molecular framework for daily rhythmic stomatal movements under well-watered conditions, whereby PIFs are required for accumulation of KAT1 at night, which upon activation by blue light in the morning leads to the K+ intake driving stomata opening.

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Section: Methodsmentioning

confidence: 99%

PIF transcriptional regulators are required for rhythmic stomatal movements

Rovira,

Veciana,

Basté-Miquel

et al. 2024

Nat Commun

Self Cite

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The ascorbate biosynthesis pathway in plants is known, but there is a way to go with understanding control and functions

Smirnoff,

Wheeler

2024

Journal of Experimental Botany

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Ascorbate (vitamin C) is one of the most abundant primary metabolites in plants. Its complex chemistry enables it to function as an antioxidant, free radical scavenger and as a reductant for iron and copper. Ascorbate biosynthesis occurs via the mannose/L-galactose pathway in green plants and the evidence for this pathway being the major route is reviewed. Ascorbate accumulation is leaves is responsive to light, reflecting various roles in photoprotection. GDP-L-galactose phosphorylase (GGP) is the first dedicated step in the pathway and is important in controlling ascorbate synthesis. Its expression is determined by a combination of transcription and translation. Translation is controlled by an upstream open reading frame (uORF) which blocks translation of the main GGP coding sequence, possibly in an ascorbate-dependent manner. GGP associates with a PAS-LOV protein, inhibiting its activity and dissociation is induced by blue light. While low ascorbate mutants are susceptible to oxidative stress, they grow nearly normally. In contrast, mutants lacking ascorbate do not grow unless rescued by supplementation. Further research should investigate possible basal functions of ascorbate in severely deficient plants involving prevention of iron over-oxidation in 2-oxoglutarate-dependent dioxygenases and iron mobilisation during seed development and germination.

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Maternal Embryo Effect Arrest 31 (MEE31) is a moonlighting protein involved in GDP-D-mannose biosynthesis and KAT1 potassium channel regulation

Cited by 2 publications

References 73 publications

PIF transcriptional regulators are required for rhythmic stomatal movements

PIF transcriptional regulators are required for rhythmic stomatal movements

The ascorbate biosynthesis pathway in plants is known, but there is a way to go with understanding control and functions

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