Maternal Liver Damage Delays Meiotic Resumption in Bovine Oocytes through Impairment of Signalling Cascades Originated from Low p38MAPK Activity in Cumulus Cells

Tanaka, Hiroshi; Takeo, Shun; Monji, Yasunori; Kuwayama, Takehito; Iwata, Hisataka

doi:10.1111/rda.12235

Cited by 5 publications

(6 citation statements)

References 58 publications

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“…COCs were culture in medium containing 0 or 10 µM of MG132 and after maturation periods oocytes were denuded from the granulosa cells and subjected to western blot analysis. To quantify ubiquitinated protein, 40 oocytes were lysed in 20 µL of Laemmli sample buffer (Bio-Rad Laboratories Inc., Hercules, CA, USA) and analyzed by western blot, using a method described previously [25] with slight modifications. Briefly, rabbit polyclonal ubiquitin primary antibody (1∶1000; #3933; Cell Signaling Technology) was diluted using immunoreaction enhancer solution 1 (Toyobo, Osaka Japan), And donkey anti-rabbit IgG HRP-linked secondary antibody (1∶20 000; Abcam, Tokyo, Japan) was diluted in immunoreaction enhancer solution 2.…”

Section: Methodsmentioning

confidence: 99%

Relationship between mitochondrial DNA Copy Number and SIRT1 Expression in Porcine Oocytes

et al. 2014

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The present study assessed the effect of resveratrol on the expression of SIRT1 and mitochondrial quality and quantity in porcine oocytes. Supplementing the maturation medium with 20 µM resveratrol increased the expression of SIRT1, and enhanced mitochondrial functions, as observed from the increased ATP content and mitochondrial membrane potential. Addition of resveratrol also improved the ability of oocytes to develop into the blastocyst stage following activation. The effects of resveratrol on mitochondrial number were examined by comparing the mitochondrial DNA copy number (Mt number) between group of oocytes collected from the same donor gilt ovaries. Supplementing the maturation medium with only resveratrol did not affect the Mt number in the oocytes. However, supplementing the maturation medium with 10 µM MG132, a proteasome inhibitor, significantly increased the amount of ubiquitinated proteins and Mt number by 12 and 14%, respectively. In addition, when resveratrol was added to the medium containing MG132, the Mt number increased significantly by 39%, this effect was diminished by the addition of the SIRT1 inhibitor EX527. Furthermore, supplementing the medium with MG132 and EX527 did not affect Mt number. The mean SIRT1 expression in 20 oocytes was significantly and positively correlated with the Mt number in oocytes collected from the same donor. This study suggests that the expression of SIRT1 is associated with the Mt number in oocytes. In addition, activation of SIRT1 by resveratrol enhances the biosynthesis and degradation of mitochondria in oocytes, thereby replenishing and improving mitochondrial function and the developmental ability of oocytes.

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Section: Methodsmentioning

confidence: 99%

Relationship between mitochondrial DNA Copy Number and SIRT1 Expression in Porcine Oocytes

et al. 2014

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“…Moreover, Tanaka et al . [ 47 ] showed that delayed meiotic maturation of oocytes in dairy cows with damaged livers is induced by slow gap junctional communication closure between oocytes and cumulus cells, which results in lower fertility. Furthermore, cows with damaged livers exhibit lower concentrations of growth factors in their follicular fluid, compared with those of cows with healthy livers [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Influence of hepatic load from far-off dry period to early postpartum period on the first postpartum ovulation and accompanying subsequent fertility in dairy cows

Kawashima

Ito

Nagashima

et al. 2016

Journal of Reproduction and Development

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The aim of the present study was to investigate nutritional and metabolic parameters during the dry and early postpartum periods of ovulatory and anovulatory cows, as well as their postpartum reproductive performance. Blood samples from 20 multiparous Holstein cows were collected once a week from the far-off dry period to 3 weeks postpartum. Early postpartum (0–3 weeks) ovulation was confirmed using plasma progesterone concentration profiles, and cows were considered ovulatory if they had resumed luteal activity by this point (n = 9), whereas cows that had not were considered anovulatory (n = 11). Data from the ovulatory and anovulatory cows were analyzed separately for the far-off dry period (7–4 weeks prepartum), the close-up dry period (3–1 weeks prepartum), and the early postpartum period (0–3 weeks). Serum gamma-glutamyl transpeptidase activity (far-off, P = 0.065; close-up, P = 0.051; and early postpartum, P = 0.030) and aspartate aminotransferase (close-up, P = 0.050 and early postpartum, P = 0.087) activities were higher in anovulatory than in ovulatory cows. The days open period was longer (P = 0.019) in anovulatory than in ovulatory cows, and the number of artificial inseminations per conception (P = 0.025) was greater. In conclusion, we found that continuously high gamma-glutamyl transpeptidase activities in serum, which may be induced by liver disorders, prevent subsequent ovulation and affect subsequent fertility, even if cows obtain sufficient ovulation-related energy and β-carotene.

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“…CG distribution is a marker of oocyte cytoplasmic maturation, and the distribution pattern is reportedly regulated by microfilaments whose polymerization depends on ATP [ 28 , 32 , 33 ]; this suggests that low ATP content during oocyte maturation prevents proper CG migration. In addition, we previously showed that the timing of GV breakdown was delayed in oocytes derived from DL cows, whereas the final maturation rates were similar in oocytes from HL and DL cows [ 7 , 8 ]. Thus, the short duration of nuclear maturation may limit the proper distribution of CGs.…”

Section: Discussionmentioning

confidence: 99%

“…The diagnosis of liver conditions and donor cow selection were conducted as described in our previous report [ 7 , 8 ]. Briefly, the liver conditions were visually diagnosed based on the surface and sectional appearance by onsite veterinarians at the slaughterhouse.…”

Section: Methodsmentioning

confidence: 99%

“…Although the relationship between the negative energy condition and infertility has been reported previously [ 2 , 6 ], few studies have examined the relationship between liver damage and the low quality of bovine oocytes [ 7 , 8 , 9 , 10 ]. In these studies, one of the prominent features of oocytes derived from cows with liver damage was the high frequency of abnormal fertilization [ 8 ]; however, the molecular mechanism underlying fertilization abnormalities are unclear.…”

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confidence: 99%

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Liver condition of Holstein cows affects mitochondrial function and fertilization ability of oocytes

Tanaka

Takeo

Abe

et al. 2016

Journal of Reproduction and Development

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The aim of the present study was to examine the fertilization ability and mitochondrial function of oocytes derived from cows with or without liver damage. Oocytes were collected from the ovaries of cows with damaged livers (DL) and those of cows with healthy livers (HL), subjected to in vitro maturation, and fertilized in vitro. A significantly high abnormal fertilization rate was observed for oocytes from DL cows compared to oocytes from HL cows. The time to dissolve the zona pellucida by protease before fertilization was similar between the two liver conditions, whereas after fertilization treatment this time was shorter for DL cows than for HL cows. The percentage of oocytes with equivalent cortical granule distributions underneath the membrane was greater for in vitro matured oocytes from HL cows, whereas an immature distribution pattern was observed for oocytes from DL cows. In addition, a greater percentage of oocytes derived from HL cows released cortical granules following fertilization compared with oocytes from DL cows. Mitochondrial function determined by ATP content and membrane potential were similar at the germinal vesicle stage, but post-in vitro maturation, the oocytes derived from HL cows showed higher values than DL cows. The mitochondrial DNA copy number in oocytes was similar between the two liver conditions for both the germinal vesicle and post-in vitro maturation oocytes. In conclusion, liver damage induces low fertilization, likely because of incomplete cortical granule distribution and release, and the maturation of oocytes from DL cows contain low-functioning mitochondria compared to their HL counterparts.

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Maternal Liver Damage Delays Meiotic Resumption in Bovine Oocytes through Impairment of Signalling Cascades Originated from Low p38MAPK Activity in Cumulus Cells

Cited by 5 publications

References 58 publications

Relationship between mitochondrial DNA Copy Number and SIRT1 Expression in Porcine Oocytes

Relationship between mitochondrial DNA Copy Number and SIRT1 Expression in Porcine Oocytes

Influence of hepatic load from far-off dry period to early postpartum period on the first postpartum ovulation and accompanying subsequent fertility in dairy cows

Liver condition of Holstein cows affects mitochondrial function and fertilization ability of oocytes

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