Maternal oxidized soybean oil exposure in rats during lactation damages offspring kidneys via Nrf2/HO‐1 and NF‐κB signaling pathway

Abstract

BACKGROUND Cooking oil is an indispensable component of the human diet. However, oils usually undergo thermal oxidation. Oxidized soybean oil (OSO) has been shown to have detrimental effects on humans and has emerged as a root cause of many chronic diseases. The objective of this work was to evaluate the effects of puerpera exposure to OSO on kidney damage in the mother and offspring using lactating rats as an experimental model. RESULTS Pathological sections and ultrastructure showed that OSO exposure resulted in various levels of damage to lactating rats and their offspring. OSO induced oxidative stress in the kidneys of lactating rats, as evidenced by increased levels of hydrogen peroxide, interleukin (IL)‐1β, and IL‐8. OSO increased the activities of glutathione peroxidase and superoxide dismutase. OSO upregulated the expression of apoptosis‐related genes, nuclear factor‐erythroid 2‐related factor 2 (Nrf2), and nuclear factor κB‐related inflammatory factor genes. In the offspring of the OSO‐exposed mothers, hydrogen peroxide, malondialdehyde, IL‐6, and tumor necrosis factor‐alpha contents were increased. Furthermore, OSO enhanced the levels of Nrf2, NAD(P)H quinone oxidoreductase 1, heme oxygenase 1, and p65 and decreased B‐cell lymphoma 2. CONCLUSION These findings indicated that the kidneys of two generations of rats were compromised by oxidative damage when fed OSO during lactation. This study provides evidence for increasing the genes expression of the Nrf2/heme oxygenase 1 pathway to alleviate the kidney damage caused by OSO in the mother and offspring. © 2021 Society of Chemical Industry.

Oxidized Soybean Oil Evoked Hepatic Fatty Acid Metabolism Disturbance in Rats and their Offspring

“…Studies have shown that LXR deletion not only interfered with the gestation adaptation of triglyceride metabolism in the liver but also caused abnormal fetal placental dyslipidemia due to the increased decomposition of maternal white fat . Meanwhile, in previous studies, we found that female rats supplementing oxidized oil containing high concentrations of lipid peroxidation products would affect the development and antioxidant status of fetuses and suckling pups. , In this experiment, F1 offspring showed the same trend as gestational F0 dams with increased oxidation degree. The mRNA levels of lipid synthesis-related genes LXRα, PPARγ, SREBP-1c, SCD-1, ACC, FAS, and CD36 were reduced.…”

“…Furthermore, our prior study found that exposure of rats to OSO during lactation will affect the kidney health of their offspring . It was also found in our study that a similar phenomenon occurred.…”

“…26 Furthermore, our prior study found that exposure of rats to OSO during lactation will affect the kidney health of their offspring. 27 It was also found in our study that a similar phenomenon occurred. We found in the pathological structure of the offspring's liver that as the degree of oxidation increased, the liver of F1 rats at 21 days and 56 days contained lipid vacuoles, and the liver sinusoids gradually widened.…”

“…56 Meanwhile, in previous studies, we found that female rats supplementing oxidized oil containing high concentrations of lipid peroxidation products would affect the development and antioxidant status of fetuses and suckling pups. 21,27 In this experiment, F1 offspring showed the same trend as gestational F0 dams with increased oxidation degree. The mRNA levels of lipid synthesis-related genes LXRα, PPARγ, SREBP-1c, SCD-1, ACC, FAS, and CD36 were reduced.…”

Effects of oxidized soybean oil on the performance of sows and jejunum health of suckling piglets

“…Fresh soybean oil (POV = 11.5 mEqO 2 kg −1 ) was bubbled with air at 65°C with a 4 L min −1 until the POV reached 220 mEqO 2 kg −1 . POV was determined by the previously described method (Gao et al, 2021). The POV was 11.5 mEqO 2 kg −1 for the fresh soybean oil group (FSO) and 220 mEqO 2 kg −1 for the oxidized soybean oil group (OSO).…”