Maternal propionate supplementation ameliorates glucose and lipid metabolic disturbance in hypoxia-induced fetal growth restriction

Chen, Dan; Wang, Yingying; Li, Sheng-peng; Zhao, Huimin; Jiang, Feng; Wu, Yaxian; Ying, Tiejin; Pang, Qingfeng

doi:10.1039/d2fo01481e

Cited by 13 publications

(9 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the top enriched GO terms were immunologically and metabolically relevant, with results for the former similar to previous findings ( Yang et al, 2021 ). These results are consistent with the occurrence of aberrant immune regulation ( Sato, 2022 ) and fatty acid metabolism ( Chen et al, 2022 ) in the pathogenesis of FGR and suggest that placental eccDNA plays a role in FGR. Six of the 10 top differentially abundant placental eccDNA molecules were successfully validated by Sanger sequencing, a non-optimal result but similar to those obtained in previous research ( Kumar et al, 2020 ; Cen et al, 2022 ), which reflects the gap between next-generation sequencing technologies and Sanger sequencing as the traditional gold standard.…”

Section: Discussionsupporting

confidence: 87%

Integrative profiling of extrachromosomal circular DNA in placenta and maternal plasma provides insights into the biology of fetal growth restriction and reveals potential biomarkers

et al. 2023

View full text Add to dashboard Cite

Introduction: Fetal growth restriction (FGR) is a placenta-mediated pregnancy complication that predisposes fetuses to perinatal complications. Maternal plasma cell-free DNA harbors DNA originating from placental trophoblasts, which is promising for the prenatal diagnosis and prediction of pregnancy complications. Extrachromosomal circular DNA (eccDNA) is emerging as an ideal biomarker and target for several diseases.Methods: We utilized eccDNA sequencing and bioinformatic pipeline to investigate the characteristics and associations of eccDNA in placenta and maternal plasma, the role of placental eccDNA in the pathogenesis of FGR, and potential plasma eccDNA biomarkers of FGR.Results: Using our bioinformatics pipelines, we identified multi-chromosomal-fragment and single-fragment eccDNA in placenta, but almost exclusively single-fragment eccDNA in maternal plasma. Relative to that in plasma, eccDNA in placenta was larger and substantially more abundant in exons, untranslated regions, promoters, repetitive elements [short interspersed nuclear elements (SINEs)/Alu, SINEs/mammalian-wide interspersed repeats, long terminal repeats/endogenous retrovirus-like elements, and single recognition particle RNA], and transcription factor binding motifs. Placental multi-chromosomal-fragment eccDNA was enriched in confident enhancer regions predicted to pertain to genes in apoptosis, energy, cell growth, and autophagy pathways. Placental eccDNA–associated genes whose abundance differed between the FGR and control groups were associated with immunity-related gene ontology (GO) terms. The combined analysis of plasma and placental eccDNA–associated genes in the FGR and control groups led to the identification of potential biomarkers that were assigned to the GO terms of the epigenetic regulation of gene expression and nutrient-related processes, respectively.Conclusion: Together, our results highlight links between placenta functions and multi-chromosomal-fragment and single-fragment eccDNA. The integrative analysis of placental and plasma eccDNA confirmed the potential of these molecules as disease-specific biomarkers of FGR.

show abstract

Section: Discussionsupporting

confidence: 87%

Integrative profiling of extrachromosomal circular DNA in placenta and maternal plasma provides insights into the biology of fetal growth restriction and reveals potential biomarkers

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Bacteroidota abundantly produce propionate, a precursor in glucose, lipid, and protein synthesis. This metabolic activity is believed to contribute to improved fetal growth and plays a role in mitigating glucose and lipid disorders in metabolic diseases [66,67]. We confirmed that the microbiome was transferred from sows to piglets and was retained until the post-weaning period.…”

Section: Discussionsupporting

confidence: 67%

Early Colonization of the Intestinal Microbiome of Neonatal Piglets Is Influenced by the Maternal Microbiome

Lim,

Cha,

Choi

et al. 2023

Animals

View full text Add to dashboard Cite

The intestinal microbiome plays a crucial role in animal health and growth by interacting with the host, inhibiting pathogenic microbial colonization, and regulating immunity. This study investigated dynamic changes in the fecal microbial composition of piglets from birth through weaning and the relationship between the piglet fecal microbiome and sows. Feces, skin, neonatal oral cavity, and vaginal samples were collected from eight sows and sixty-three piglets, and 16S genome sequencing was performed. The results revealed that Firmicutes, Bacteroidetes, and Proteobacteria dominated the piglet microbiome in the early stages, and Firmicutes and Bacteroidetes were crucial for maintaining a balance in the intestinal microbiome during nursing. The abundance of Christensenellaceae_R-7_group, Succinivibrio, and Prevotella increased in weaned piglets fed solid feed. Analysis of the microbiome from sows to piglets indicated a shift in the microbiome colonizing piglet intestines, which became a significant constituent of the piglet intestinal microbiome. This study supports the theory that the neonatal intestinal microbiome is vertically transmitted from the mother. Further research is required to integrate factors related to sows, piglets, and their environments to gain a better understanding of the early establishment of the intestinal microbiome in piglets.

show abstract

“…It has been reported that SCFAs may regulate intestinal immunity via the production of interleukin-22 by innate lymphoid cells and CD4 + T cells ( 27 ). In a recent study, supplementation of SCFAs, particularly propionate, was shown to reverse the reduction in birth weight in hypoxia-induced FGR ( 28 ). In line with these findings, our data showed that the abundance of SCFA producers Ruminococcaceae , Bacteroides uniformis , and Alistipes onderdonkii was relatively lower, coupled with the involvement of butanoate metabolism in patients with FGR ( 29 , 30 ).…”

Section: Discussionmentioning

confidence: 99%

Alterations in the Gut Microbiome and Metabolisms in Pregnancies with Fetal Growth Restriction

Tao

Chen

et al. 2023

Microbiol Spectr

View full text Add to dashboard Cite

show abstract

Maternal propionate supplementation ameliorates glucose and lipid metabolic disturbance in hypoxia-induced fetal growth restriction

Abstract: Intrauterine growth restriction (IUGR), one of the major complications of pregnancy, is characterized by the low-birth weight and results in higher risks for long-term problems including developing metabolic and cardiovascular...

Cited by 13 publications

References 50 publications

Integrative profiling of extrachromosomal circular DNA in placenta and maternal plasma provides insights into the biology of fetal growth restriction and reveals potential biomarkers

Integrative profiling of extrachromosomal circular DNA in placenta and maternal plasma provides insights into the biology of fetal growth restriction and reveals potential biomarkers

Early Colonization of the Intestinal Microbiome of Neonatal Piglets Is Influenced by the Maternal Microbiome

Alterations in the Gut Microbiome and Metabolisms in Pregnancies with Fetal Growth Restriction

Contact Info

Product

Resources

About