Maternal Serum Screening for Neural Tube and Other Defects

Milunsky, Aubrey; Canick, Jacob A.

doi:10.1002/9781444314342.ch23

Cited by 8 publications

(5 citation statements)

References 524 publications

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“…Gulbis et al (1998) describe this as a human protein having a molecular weight of 70 kDa, which is close to that found by Milunsky (1992) of 69 kDa and by Alpert et al (1972) of 72 kDa. Regarding the test done with the primary monoclonal antibody anti-mouse for human AFP (SERO-MCA1863HT -1 mL), with no reaction with the other samples, we can assume that in the other samples, there was a lack of this protein or that the primary antibody had spoiled, which was unlikely to happen.…”

Section: Protein Analysissupporting

confidence: 84%

Characterization of yolk sac proteins of Bos indicus cattle embryos

Matsumoto

Oliveira²,

Mançanares

et al. 2012

Genet. Mol. Res.

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ABSTRACT. The yolk sac is an embryonic membrane that is essential for the embryo's initial survival in many mammals. It also plays an important role in the production of proteins necessary for development. We studied proteins of the yolk sac in bovine embryos at up to 40 days of gestation. We examined the yolk sac of 17 bovine embryos at different gestational periods, measuring α-fetoprotein, α-1-antitrypsin, and transferrin. This experiment was carried out by Western blot technique, associated with electrophoresis on a 6% sodium dodecyl sulfate polyacrylamide gel. Mouse monoclonal antibody anti-human-α-fetoprotein, mouse antibody anti-human-transferrin and rabbit polyclonal anti-human-α-1-antitrypsin were used as primary antibodies, and conjugated peroxidase as a secondary antibody. We detected the three proteins in some of the yolk sac samples; however, the bands in some specimens (samples) were weak, maybe a result of poor antigenantibody reaction, since the antibodies used in this study were not Bovine yolk sac proteins specific to bovine proteins. The fact that weak bands appeared might be due to a weak cross-reaction.

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Section: Protein Analysissupporting

confidence: 84%

Characterization of yolk sac proteins of Bos indicus cattle embryos

Matsumoto

Oliveira²,

Mançanares

et al. 2012

Genet. Mol. Res.

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“…2,8 In our set up screening method by maternal serum alpha-fetoprotein is not widely available and early diagnosis does not change the outcome of this case, but obstetric ultrasonography report excellent sensitivity and specificity in detecting fetal neural tube defects, especially in high-risk patients, and many hospital or diagnostic center offer specialized ultrasound examination as a diagnostic test for women at high risk of neural tube defects. 9,10 The fetus with neural tube defect should be delivered at term when lung maturity is well established. In this case a neural tube defect was diagnosed during antenatal care follow up but delivery was delayed for achieving lung maturity of health fetus.…”

Section: Discussionmentioning

confidence: 99%

Twin pregnancy one neonate with encephalocele

Diriba¹

2018

IPCB

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Encephalocele is neural tube defect characterized by failure of the surface ectoderm to separate from the neuroectoderm. This leads to a bony defect in the skull table, which allows herniation of the meninges or brain tissue.1,2 To report case of 30 years old gravida 5 para 3 abortion 1 lady admitted with diagnosis of twin pregnancy and one of the twin with neural tube defect, delivered one health neonate and other with encephalocele. Conclusion folic acid supplementation and/or food fortification for all reproductive age groups will reduce incidence and recurrence of neural tube defect according to the level of the risk they have.

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“…Raised MSAFP levels should be offered genetic counselling and targeted ultrasound for further evaluation. [8]…”

Section: Current Screening Technologiesmentioning

confidence: 99%

Prenatal screening methods for aneuploidies

2013

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Aneuploidies are a major cause of perinatal morbidity and mortality. Therefore, it is the most common indication for invasive prenatal diagnosis. Initially, screening for aneuploidies started with maternal age risk estimation. Later on, serum testing for biochemical markers and ultrasound markers were added. Women detected to be at high-risk for aneuploidies were offered invasive testing. New research is now focusing on non-invasive prenatal testing using cell-free fetal DNA in maternal circulation. The advantage of this technique is the ability to reduce the risk of miscarriage associated with invasive diagnostic procedures. However, this new technique has its own set of technical limitations and ethical issues at present and careful consideration is required before broad implementation

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Maternal Serum Screening for Neural Tube and Other Defects

Cited by 8 publications

References 524 publications

Characterization of yolk sac proteins of Bos indicus cattle embryos

Characterization of yolk sac proteins of Bos indicus cattle embryos

Twin pregnancy one neonate with encephalocele

Prenatal screening methods for aneuploidies

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