2018
DOI: 10.15407/biotech11.02.040
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MATHEMATICAL MODELLING OF OPTIMIZATION NUTRIENT MEDIUM COMPOSITION FOR ENTOMOPATHOGENIC BACTERIA STRAIN Bacillus thuringiensis 87/3 CULTIVATION

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“…An analysis of available publications on the biosynthesis of recombinant cytokines and other proteins in the Escherichia coli expression system suggests that the most commonly used are standard mediums LB, YT and TB, as well as similar peptone-containing mediums In most cases, the authors do not provide experimental data that would confirm the substantiality of using the qualitative and quantitative media composition. Various methodological approaches may be used to determine the optimal composition of culture media, however, the most commonly used are the Box-Wilson method, complete and fractional factor experiments (Adinarayana et al, 2002;Boiko et al, 2018;Wang et al, 2009;Singh et al, 2017). In the case of a fractional factorial experiment (FFE) there is a possibility to reduce the number of experiments due to the correlation between the columns of the matrix of planning, which complicates this stage, but does not affect the validity of the estimates of the experiment.…”
Section: Introductionmentioning
confidence: 99%
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“…An analysis of available publications on the biosynthesis of recombinant cytokines and other proteins in the Escherichia coli expression system suggests that the most commonly used are standard mediums LB, YT and TB, as well as similar peptone-containing mediums In most cases, the authors do not provide experimental data that would confirm the substantiality of using the qualitative and quantitative media composition. Various methodological approaches may be used to determine the optimal composition of culture media, however, the most commonly used are the Box-Wilson method, complete and fractional factor experiments (Adinarayana et al, 2002;Boiko et al, 2018;Wang et al, 2009;Singh et al, 2017). In the case of a fractional factorial experiment (FFE) there is a possibility to reduce the number of experiments due to the correlation between the columns of the matrix of planning, which complicates this stage, but does not affect the validity of the estimates of the experiment.…”
Section: Introductionmentioning
confidence: 99%
“…In the case of a fractional factorial experiment (FFE) there is a possibility to reduce the number of experiments due to the correlation between the columns of the matrix of planning, which complicates this stage, but does not affect the validity of the estimates of the experiment. Therefore, given the literature (Adinarayana et al, 2002;Boiko et al, 2018;Wang et al, 2009;Singh et al, 2017) and the results of our own previous experiments on the biosynthesis of recombinant proteins in E. coli (including recombinant interleukin-7, rIL-7) (Galkіn et al, 2015), the concept of FFE is quite acceptable. The works of various authors testify to the possibility of effective application of plant extracts as growth stimulators of bacterial cells, as well as the synthesis of bacteria of certain products, including recombinant proteins (Pererva et al, 2012;Galkіn et al, 2015;Pererva et al, 2012).…”
Section: Introductionmentioning
confidence: 99%