Matrices and Affinity Ligands for Antibody Purification and Corresponding Applications in Radiotherapy

Abstract

Antibodies have become an important class of biological products in cancer treatments such as radiotherapy. The growing therapeutic applications have driven a demand for high-purity antibodies. Affinity chromatography with a high affinity and specificity has always been utilized to separate antibodies from complex mixtures. Quality chromatographic components (matrices and affinity ligands) have either been found or generated to increase the purity and yield of antibodies. More importantly, some matrices (mainly particles) and affinity ligands (including design protocols) for antibody purification can act as radiosensitizers or carriers for therapeutic radionuclides (or for radiosensitizers) either directly or indirectly to improve the therapeutic efficiency of radiotherapy. This paper provides a brief overview on the matrices and ligands used in affinity chromatography that are involved in antibody purification and emphasizes their applications in radiotherapy to enrich potential approaches for improving the efficacy of radiotherapy.

Implementation of Novel Affinity Ligand for Lentiviral Vector Purification

“…After selecting the final candidates, it was necessary to decide on an appropriate matrix to assemble the novel affinity prototypes. To be considered an ideal matrix, a few requisites should be met, such as homogeneity, stability, residual nonspecific binding, large surface area for ligand attachment, and easy ligand coupling [ 25 , 49 ]. Therefore, to determine the most suitable resin for functionalization with the VSVG-LVs binding ligands, a set of different matrices were compared ( Table 1 ).…”