Matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectrometry in lipid and phospholipid research

Schiller, Jürgen; Süß, Rosmarie; Arnhold, J; Fuchs, Beate; Lessig, Jacqueline; Müller, Mat­thias; Petković, Marijana; Spalteholz, Holger; Zschörnig, Olaf; Arnold, Klaus

doi:10.1016/j.plipres.2004.08.001

Cited by 356 publications

(281 citation statements)

References 140 publications

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“…The use of matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) or more specifically MALDI-time of flight mass spectrometry (MALDI-TOF) has become routine in the analysis of large molecules such as lipids, proteins, carbohydrates and nucleic acids (96)(97)(98)(99)(100)(101). It has been applied more recently to the analysis of proteins in ancient bones (102,103) and eggshells (104), where it allows the identification of species, and has been used to analyse proteinaceous and lipid binders in works of art and wall plasters (105)(106)(107)(108).…”

Section: New Mass Spectrometric Techniquesmentioning

confidence: 99%

Organic Residues in Archaeology: The Highs and Lows of Recent Research

Steele

2013

ACS Symposium Series

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The analysis of organic residues from archaeological materials has become increasingly important to our understanding of ancient diet, trade and technology. Residues from diverse contexts have been retrieved and analysed from the remains of food, medicine and cosmetics to hafting material on stone arrowheads, pitch and tar from shipwrecks, and ancient manure from soils. Research has brought many advances in our understanding of archaeological, organic residues over the past two decades. Some have enabled very specific and detailed interpretations of materials preserved in the archaeological record. However there are still areas where we know very little, like the mechanisms at work during the formation and preservation of residues, and areas where each advance produces more questions rather than answers, as in the identification of degraded fats. This chapter will discuss some of the significant achievements in the field over the past decade and the ongoing challenges for research in this area.1341280_File000001_20903114.docPrinted 7/19/2013 2

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Section: New Mass Spectrometric Techniquesmentioning

confidence: 99%

Organic Residues in Archaeology: The Highs and Lows of Recent Research

Steele

2013

ACS Symposium Series

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“…These methods also provide high sensitivity, and lipid analyses can be achieved with submicrogram samples. Comprehensive reviews of ESI-MS 16 , 17 and MALDI-TOF (time-offl ight) MS 18 and their application to lipidomics have been published.…”

Section: Lipidomicsmentioning

confidence: 99%

Lipids and lipidomics in brain injury and diseases

Adibhatla

Hatcher

Dempsey

2006

AAPS J

147

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Lipidomics is systems-level analysis and characterization of lipids and their interacting moieties. The amount of information in the genomic and proteomic fi elds is greater than that in the lipidomics fi eld, because of the complex nature of lipids and the limitations of tools for analysis. The main innovation during recent years that has spurred advances in lipid analysis has been the development of new mass spectroscopic techniques, particularly the " soft ionization " techniques electrospray ionization and matrix-assisted laser desorption/ionization. Lipid metabolism may be of particular importance for the central nervous system, as it has a high concentration of lipids. The crucial role of lipids in cell signaling and tissue physiology is demonstrated by the many neurological disorders, including bipolar disorders and schizophrenia, and neurodegenerative diseases such as Alzheimer ' s, Parkinson ' s, and Niemann-Pick diseases, that involve deregulated lipid metabolism. Altered lipid metabolism is also believed to contribute to cerebral ischemic (stroke) injury. Lipidomics will provide a molecular signature to a certain pathway or a disease condition. Lipidomic analyses (characterizing complex mixtures of lipids and identifying previously unknown changes in lipid metabolism) together with RNA silencing, using small interfering RNA (siRNA), may provide powerful tools to elucidate the specifi c roles of lipid intermediates in cell signaling and open new opportunities for drug development.K e yw o r ds: Acrolein , arachidonic acid , cerebral ischemia , cytidine-5 ′ -diphosphocholine , CDP-choline , citicoline , docosahexaenoic acid , 4-hydroxynonenal , lipidomics , lipid peroxidation , MALDI-TOF , ESI-MS-MS , neuroprotectin D1 , 10,17S-docosatriene , phospholipases , phospholipids , RNA silencing , small interfering RNA , siRNA , reactive oxygen species , stroke INTRODUCTIONPhospholipids are important components of all mammalian cells and have a variety of biological functions: (1) they form lipid bilayers that provide structural integrity necessary for protein function, (2) they function as an energy reservoir (eg, triglycerides), and (3) they serve as precursors for various second messengers such as arachidonic acid, docosahexaenoic acid, ceramide, 1,2-diacylglycerol, phosphatidic acid, and lyso-phosphatidic acid. A deeper knowledge of the complexity of phospholipid metabolism will elevate our understanding of the role of lipids in maintaining normal cell physiology and how alterations in lipid metabolism contribute to various disease states. DEFINING A LIPIDLipids comprise an enormous number of chemically distinct molecules arising from combinations of fatty acids with various backbone structures. Indeed, it is diffi cult to defi ne what lipids are. Defi ning lipids as a class of biological molecules that share a high degree of solubility in organic solvents is considered at once too broad and too narrow, encompassing structurally and functionally unrelated molecules and excluding lipids, such as ganglioside...

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“…However, the disadvantages of MALDI include the limited quantitative applications and the need to do preliminary separations if complex samples are analyzed. Schiller et al (2004) has published a comprehensive review of MALDI for the analysis of phospholipids.…”

Section: Soft Ionization Methodsmentioning

confidence: 99%

A review of chromatographic methods for the assessment of phospholipids in biological samples

Peterson

Cummings

2005

Biomedical Chromatography

235

159

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Phospholipids are important constituents of all living cell membranes. Lipidomics is a rapidly growing field that provides insight as to how specific phospholipids play roles in normal physiological and disease states. There are many analytical methods available for the qualitative and quantitative determination of phospholipids. This review provides a summary of the methods that were historically used such as thin layer chromatography, gas chromatography and high-performance liquid chromatography. In addition, an introduction to applications of interfacing these traditional chromatographic techniques with mass spectrometry is provided.

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Matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectrometry in lipid and phospholipid research

Cited by 356 publications

References 140 publications

Organic Residues in Archaeology: The Highs and Lows of Recent Research

Organic Residues in Archaeology: The Highs and Lows of Recent Research

Lipids and lipidomics in brain injury and diseases

A review of chromatographic methods for the assessment of phospholipids in biological samples

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