Matrix-Assisted Laser Desorption/Ionization (MALDI) Imaging Mass Spectrometry (IMS): A Challenge for Reliable Quantitative Analyses

Kubo, Akiko; Kajimura, Mayumi; Suematsu, Makoto

doi:10.5702/massspectrometry.a0004

Cited by 14 publications

(15 citation statements)

References 48 publications

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“…Tissue washing was refused in this study, as hydrophilic solvents would have caused metabolite diffusion and high amylase activity [ 58 ]. MS signal intensities were not suitable for absolute quantification, which is still a challenge in MALDI MSI [ 59 ]. The use of labeled external standards might be beneficial to address the quantification of oligosaccharides.…”

Section: Discussionmentioning

confidence: 99%

Spatio-Temporal Metabolite Profiling of the Barley Germination Process by MALDI MS Imaging

Gorzolka

Kölling

Nattkemper³

et al. 2016

PLoS ONE

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MALDI mass spectrometry imaging was performed to localize metabolites during the first seven days of the barley germination. Up to 100 mass signals were detected of which 85 signals were identified as 48 different metabolites with highly tissue-specific localizations. Oligosaccharides were observed in the endosperm and in parts of the developed embryo. Lipids in the endosperm co-localized in dependency on their fatty acid compositions with changes in the distributions of diacyl phosphatidylcholines during germination. 26 potentially antifungal hordatines were detected in the embryo with tissue-specific localizations of their glycosylated, hydroxylated, and O-methylated derivates. In order to reveal spatio-temporal patterns in local metabolite compositions, multiple MSI data sets from a time series were analyzed in one batch. This requires a new preprocessing strategy to achieve comparability between data sets as well as a new strategy for unsupervised clustering. The resulting spatial segmentation for each time point sample is visualized in an interactive cluster map and enables simultaneous interactive exploration of all time points. Using this new analysis approach and visualization tool germination-dependent developments of metabolite patterns with single MS position accuracy were discovered. This is the first study that presents metabolite profiling of a cereals’ germination process over time by MALDI MSI with the identification of a large number of peaks of agronomically and industrially important compounds such as oligosaccharides, lipids and antifungal agents. Their detailed localization as well as the MS cluster analyses for on-tissue metabolite profile mapping revealed important information for the understanding of the germination process, which is of high scientific interest.

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Section: Discussionmentioning

confidence: 99%

Spatio-Temporal Metabolite Profiling of the Barley Germination Process by MALDI MS Imaging

Gorzolka

Kölling

Nattkemper³

et al. 2016

PLoS ONE

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“…Matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS) is a technology that has been used for visualizing in situ distributions of small metabolites (19). It has been used for examining tissue distributions of substances, which are difficult for immunostaining techniques to detect (20)(21)(22)(23).…”

Section: Introductionmentioning

confidence: 99%

Xanthine oxidase inhibitor ameliorates postischemic renal injury in mice by promoting resynthesis of adenine nucleotides

Fujii¹,

Kubo

Miyashita³

et al. 2019

JCI Insight

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“…MALDI has become widely used because it is among the most sensitive, highest throughput and highest spatial resolution of MSI techniques. A number of previous reviews have covered various aspects and accumulated literature of MALDI-MSI in detail, including the quantitative analysis of tissue [53][54][55][56][57][58][59][60][61][62][63]. Here, we briefly overview the approaches and challenges to achieving quantitative MSI with MALDI, which is non-trivial and requires care in experimental design.…”

Section: Tissue Imagingmentioning

confidence: 99%

Spatially resolved absolute quantitation in thin tissue by mass spectrometry

Kertész

Cahill

2020

Anal Bioanal Chem

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Mass spectrometry (MS) has become the de facto tool for routine quantitative analysis of biomolecules. MS is increasingly being used to reveal the spatial distribution of proteins, metabolites, and pharmaceuticals in tissue and interest in this area has led to a number of novel spatially resolved MS technologies. Most spatially resolved MS measurements are qualitative in nature due to a myriad of potential biases, such as sample heterogeneity, sampling artifacts, and ionization effects. As applications of spatially resolved MS in the pharmacological and clinical fields increase, demand has become high for quantitative MS imaging and profiling data. As a result, several varied technologies now exist that provide differing levels of spatial and quantitative information. This review provides an overview of MS profiling and imaging technologies that have demonstrated quantitative analysis from tissue. Focus is given on the fundamental processes affecting quantitative analysis in an array of MS imaging and profiling technologies and methods to address these biases.

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Matrix-Assisted Laser Desorption/Ionization (MALDI) Imaging Mass Spectrometry (IMS): A Challenge for Reliable Quantitative Analyses

Cited by 14 publications

References 48 publications

Spatio-Temporal Metabolite Profiling of the Barley Germination Process by MALDI MS Imaging

Spatio-Temporal Metabolite Profiling of the Barley Germination Process by MALDI MS Imaging

Xanthine oxidase inhibitor ameliorates postischemic renal injury in mice by promoting resynthesis of adenine nucleotides

Spatially resolved absolute quantitation in thin tissue by mass spectrometry

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