Matrix-Assisted Laser Desorption Ionization - Time of Flight Mass Spectrometry: An Emerging Tool for the Rapid Identification of Mosquito Vectors

Yssouf, Amina; Socolovschi, Cristina; Flaudrops, Christophe; Ndiath, Mamadou Ousmane; Sougoufara, Seynabou; Dehecq, Jean-Sébastien; Lacour, Guillaume; Bérenger, Jean-Michel; Sokhna, Cheikh; Raoult, Didier; Parola, Philippe

doi:10.1371/journal.pone.0072380

Cited by 114 publications

(194 citation statements)

References 37 publications

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“…Protein profiling using MALDI-TOF MS was developed during the last decade as an important tool for the identification of microorganisms (20). Furthermore, it is a powerful method and is sufficiently reproducible and sensitive enough to rapidly survey the evolution of existing or emerging phenotypes with reduced financial and human costs (4 In the current study, the bioMérieux VITEK MS system was able to identify the Klebsiella species with an accuracy of 100%, and the reliability of some results was confirmed by sequencing analysis.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Klebsiella isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and determination of antimicrobial resistance with VITEK 2 advanced expert system (AES)

Karagöz¹,

Acar²,

Körkoca³

2015

Turk J Med Sci

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Background/aim: The purpose of the study was to evaluate the performance of the VITEK mass spectrometry (MS) (bioMérieux, France) system for the identification of Klebsiella spp. isolated from different sources. Moreover, while assessing the ability of the VITEK 2 automated expert system (AES) to recognize antimicrobial resistance patterns, the researchers have extended the study to compare VITEK 2 with the routine antimicrobial susceptibility testing method. Materials and methods:This study tested 51 Klebsiella spp. isolates that were isolated from environmental examples and clinical examples. Results of conventional methods and the matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS were compared. Then, any differing results were compared against a reference 16S rRNA gene sequence, and when indicated, a recA sequencing analysis was done.Results: VITEK MS correctly identified 100% of the Klebsiella spp. isolates. There were two K. oxytoca isolates incorrectly identified to the species level with conventional methods according to the 16S rRNA gene sequencing analysis. In addition, a VITEK 2 AST-N261 card was used for the detection of extended spectrum beta-lactamases (ESBL). Using the VITEK 2 AES, ESBL positivity was found at the rate of 16.3% whereas this rate was 4.08% using the disk diffusion method. Conclusion:MALDI-TOF MS is a rapid and accurate method for the identification of Klebsiella spp. Moreover, the bioMérieux AES provides a useful laboratory tool for the interpretation of susceptibility results.

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Section: Discussionmentioning

confidence: 99%

Characterization of Klebsiella isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and determination of antimicrobial resistance with VITEK 2 advanced expert system (AES)

Karagöz¹,

Acar²,

Körkoca³

2015

Turk J Med Sci

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“…5,18,[31][32][33][34][35][36][37][38] Moreover, MALDI-TOF MS has enabled the rapid detection of tick and mosquito vectors without requiring previous expertise in entomology. 39,40 Thus, this technique will be of use in the implementation of effective prevention measures for vector-borne diseases. 39,40 In the future, it will be useful for tropical countries to evaluate whether MALDI-TOF MS may be used to distinguish between uninfected mosquitoes and those infected with sporozoites of Plasmodium spp.…”

Section: Discussionmentioning

confidence: 99%

“…39,40 Thus, this technique will be of use in the implementation of effective prevention measures for vector-borne diseases. 39,40 In the future, it will be useful for tropical countries to evaluate whether MALDI-TOF MS may be used to distinguish between uninfected mosquitoes and those infected with sporozoites of Plasmodium spp. and determine potential vector resistance to insecticides.…”

Section: Discussionmentioning

confidence: 99%

The Ongoing Revolution of MALDI-TOF Mass Spectrometry for Microbiology Reaches Tropical Africa

Fall¹,

Lo²,

Samb-Ba³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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Abstract. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) represents a revolution in routine pathogen identification in clinical microbiology laboratories. A MALDI-TOF MS was introduced to tropical Africa in the clinical microbiology laboratory of the Hô pital Principal de Dakar (Senegal) and used for routine pathogen identification. Using MS, 2,429 bacteria and fungi isolated from patients were directly assayed, leading to the identification of 2,082 bacteria (85.7%) and 206 fungi (8.5%) at the species level, 109 bacteria (4.5%) at the genus level, and 16 bacteria (0.75%) at the family level. Sixteen isolates remained unidentified (0.75%). Escherichia coli was the most prevalent species (25.8%) followed by Klebsiella pneumoniae (14.8%), Streptococcus agalactiae (6.2%), Acinetobacter baumannii (6.1%), Pseudomonas aeruginosa (5.9%), and Staphylococcus aureus (5.9%). MALDI-TOF MS has also enabled the detection of rare bacteria and fungi. MALDI-TOF MS is a powerful tool for the identification of bacterial and fungal species involved in infectious diseases in tropical Africa.

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“…Mass spectrometry is used for numerous applications in different areas (50). Insect identification using MALDI-TOF MS is a novel entomological tool that has been utilized in only a few studies (3,37,38,40,51), including a very recent application for the identification of cryptic Anopheles mosquito species (39). Kaufmann et al applied this proteomic approach to characterize a hematophagous Culicoides species from Europe (41).…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, this proteomic approach has been used for the identification of metazoans, such as fish species (33), plants (lentil varieties) (34), ticks (35,36), and insects, such as Drosophila fruit flies (37,38) and mosquitoes (39,40).…”

mentioning

confidence: 99%

Comparison of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Molecular Biology Techniques for Identification of Culicoides (Diptera: Ceratopogonidae) Biting Midges in Senegal

et al. 2015

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Biting midges of the genus Culicoides are implicated as vectors for a wide variety of pathogens. The morphological identification of these arthropods may be difficult because of a lack of detailed investigation of taxonomy for this species in Africa. However, matrix-assisted laser desorption ionization؊time of flight mass spectrometry (MALDI-TOF MS) profiling is efficient for arthropod identification at the species level. This study established a spectrum database of Culicoides spp. from Senegal using MALDI-TOF. Identification of Culicoides insects to the species level before mass spectrometry was performed on the basis of morphological characters. MALDI-TOF MS reference spectra were determined for 437 field-caught Culicoides of 10 species. The protein profiles of all tested Culicoides revealed several peaks with mass ranges of 2 to 20 kDa. In a validation study, 72 Culicoides specimens in the target species were correctly identified at the species level with a similarity of 95 to 99.9%. Four Culicoides protein profiles were misidentified. Nevertheless, six SuperSpectra (C. imicola, C. enderleini, C. oxystoma, C. kingi, C. magnus, and C. fulvithorax) were created. Abdomens of midges were used to amplify and sequence a portion of the mitochondrial cytochrome oxidase I gene (COI). The results obtained using the MALDI-TOF MS method were consistent with the morphological identification and similar to the genetic identification. Protein profiling using MALDI-TOF is an efficient approach for the identification of Culicoides spp., and it is economically advantageous for approaches that require detailed and quantitative information of vector species that are collected in field. The database of African Culicoides MS spectra created is the first database in Africa. The COI sequences of five Culicoides species that were previously noncharacterized using molecular methods were deposited in GenBank. Biting midges of the genus Culicoides are among the smallest hematophagous insects, and they can be a nuisance to humans and animals (1). These insects are implicated as vectors for a wide variety of viruses, such as bluetongue virus (2), African horse sickness virus (3), epizootic hemorrhagic disease virus (4), the Schmallenberg virus that recently emerged in Europe (5-7), Toggenburg orbivirus (8), and the Oropouche virus (9), which is the only human virus transmitted by Culicoides. Additionally, biting midges are involved in the transmission of various human filarial parasites, such as Mansonella perstans (10-13), Mansonella streptocerca, Mansonella ozzardi, and Mansonella rodhaini (14). Recently, Leishmania spp. and Leishmania infantum were detected in Culicoides imicola and Culicoides spp. (15).Vector identification is an important step in the epidemiology of vector-borne diseases. Information on the major vector species may provide a clearer indication of the geographic distribution of the disease or its potential distribution, the location of danger points for high risks of contact with the vector, and access to alternative...

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Matrix-Assisted Laser Desorption Ionization - Time of Flight Mass Spectrometry: An Emerging Tool for the Rapid Identification of Mosquito Vectors

Cited by 114 publications

References 37 publications

Characterization of Klebsiella isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and determination of antimicrobial resistance with VITEK 2 advanced expert system (AES)

Characterization of Klebsiella isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and determination of antimicrobial resistance with VITEK 2 advanced expert system (AES)

The Ongoing Revolution of MALDI-TOF Mass Spectrometry for Microbiology Reaches Tropical Africa

Comparison of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Molecular Biology Techniques for Identification of Culicoides (Diptera: Ceratopogonidae) Biting Midges in Senegal

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