Matrix Metalloproteinase 2 in Reduced‐Size Liver Transplantation: Beyond the Matrix

Padrissa-Altés, Susagna; Zaoualí, Mohamed Amine; Franco-Gou, Rosa; Bartrons, Ramón; Boillot, Olivier; Rimola, Antoni; Arroyo, Vicente; Rodés, Joan; Peralta, Carmen; Roselló‐Catafau, Joan

doi:10.1111/j.1600-6143.2010.03092.x

Cited by 17 publications

(15 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Survival in the 60% hepatectomy and 40% SOLT groups seemed to be higher than in the 100% OLT group and this may reflect the damage induced by shear stress and portal hypertension. Our histopathological, immunohistological and biochemical findings revealed that liver damage and apoptotic induction were observed in the early postoperative period after liver surgery, as in previous studies [3,12,13,18,[29][30][31] . Paradoxically, the early postoperative period may have a therapeutic potential for a subsequent course after liver surgery.…”

Section: Discussionsupporting

confidence: 66%

“…The survival study was performed on 10 rats in each group. Cell signaling involved in proliferation, differentiation and apoptosis was confirmed at the early postoperative period after liver surgery and subsequently progressive necrosis was observed, as described previously [3,12,13,18,[29][30][31] . Serum and plasma were collected at 6 h after surgery (n = 5, in each group).…”

Section: Methodsmentioning

confidence: 99%

“…MMPs have been intensively studied and shown to play key roles in inflammation, carcinogenesis and regeneration and many researchers have already focused on MMP-2 and MMP-9 after liver surgery [12][13][14][15][16][17][18][19][20][21] . In the present study, 40% SOLT increased protein expression of MMP-2 in western blotting, although zymography did not show any differences.…”

Section: Survival Ratementioning

confidence: 99%

“…Although shear stress with portal hypertension and CIWR injury trigger the liver regeneration cascade after liver surgery, these injuries also cause fatal liver damage [29][30][31] . Initial damage is confirmed at the early postoperative period after liver surgery [3,12,13,18,[29][30][31] . Therapeutic strategies to reduce this damage have the advantage of improving clinical results after liver surgery and overcoming the current issue of insufficient liver volume in the field of liver surgery.…”

Section: Introductionmentioning

confidence: 99%

“…MMP-2 and MMP-9 are implicated in liver injury and remodeling. In particular, previous researchers reported that MMP-9 and MMP-2 contribute to liver failure after liver surgery [12][13][14][15][16][17][18][19][20][21] . Tissue inhibitors of metalloproteinases (TIMPs) are a family of endogenous inhibitors of MMPs.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Oxidative stress and extracellular matrices after hepatectomy and liver transplantation in rats

Hori¹,

Üemoto²,

Chen³

et al. 2014

WJH

View full text Add to dashboard Cite

AIM:To investigate oxidative stress (OS)-mediated damage and the behavior of extracellular matrices in various rat models because shear stress with portal hypertension and cold ischemia/warm reperfusion injury trigger the liver regeneration cascade after surgery. These injuries also cause fatal liver damage. METHODS:Rats were divided into four groups according to the surgery performed: control; hepatectomy with 40% liver remnant (60% hepatectomy); orthotopic liver transplantation (OLT) with whole liver graft (100% OLT); and split OLT (SOLT) with 40% graft (40% SOLT). Survival was evaluated. Blood and liver samples were collected at 6 h after surgery. Biochemical and histopathological examinations were performed. OSinduced damage, 4-hydroxynonenal, ataxia-telangiectasia mutated kinase, histone H2AX, phosphatidylinositol 3-kinase (PI3K) and Akt were evaluated by western blotting. Behavior of extracellular matrices, matrix metalloproteinase (MMP)-9, MMP-2, tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2 were also evaluated by western blotting and zymography. RESULTS:Although 100% OLT survived, 60% hepatectomy and 40% SOLT showed poor survival. Histopathological, immunohistological, biochemical and protein assays revealed that 60% hepatectomy, 100% OLT and 40% SOLT showed liver damage. PI3K and Akt were decreased in 60% hepatectomy and 40% SOLT. For protein expression, 40% SOLT showed differences in MMP-9, MMP-2 and TIMP-2. TIMP-1 showed differences in 60% hepatectomy and 40% SOLT. For protein activity, MMP-9 demonstrated significant differences in 60% hepatectomy, 100% OLT and 40% SOLT. CONCLUSION: Under conditions with an insufficient liver remnant, prevention of OS-induced damage viathe Akt/PI3K pathway may be key to improve the postoperative course. MMP-9 may be also a therapeutic target after surgery. Hori T et al . Hepatic damage after liver surgeryCore tip: Although shear stress with portal hypertension and cold ischemia/warm reperfusion injury trigger the liver regeneration cascade after surgery, these injuries also cause fatal liver damage. Postoperative liver damage is still a critical matter in the field of liver surgery. Oxidative stress and extracellular matrices are important for liver regeneration after surgery and these may be important keys to overcome current problems in the field of liver surgery. Here, we investigated oxidative stress-mediated damage and the behavior of extracellular matrices in various rat models with liver surgery.Hori T, Uemoto S, Chen F, Gardner LB, Baine AMT, Hata T, Kogure T, Nguyen JH. ��idative stress and e�tracellular ma��idative stress and e�tracellular matrices after hepatectomy and liver transplantation in rats. World

show abstract

Section: Discussionsupporting

confidence: 66%

Section: Methodsmentioning

confidence: 99%

Section: Survival Ratementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Oxidative stress and extracellular matrices after hepatectomy and liver transplantation in rats

Hori¹,

Üemoto²,

Chen³

et al. 2014

WJH

View full text Add to dashboard Cite

show abstract

Hepatic arterial reconstruction for orthotopic liver transplantation in the rat

Hori

Gardner

Chen

et al. 2012

Journal of Surgical Research

View full text Add to dashboard Cite

Background Orthotopic liver transplantation (OLT) models in rats have been investigated in many studies. The reconstruction of hepatic artery is required for reliable OLT and also requires advanced skills. Methods The hepatic artery reconstructions by a hand-suture technique and a new method using a micro T-tube were investigated in rats with a whole-liver syngeneic graft. Operative time and postoperative patency were compared between the hand-suture and micro T-tube techniques. Results Our technique using the micro T-tube shortened the operative time of recipient surgery compared with the hand-suture technique and prolonged the operative time for the donor. The patency ratio was maintained at 24 h after OLT with hand suturing but was significantly reduced with the micro T-tube, which had a patency ratio of 0.83 only up to 6 h after OLT. Conclusion The micro T-tube technique may have potential usefulness in the rat OLT model but requires further modification.

show abstract

Liver graft pretreated in vivo or ex vivo by γ-aminobutyric acid receptor regulation

Hori

Gardner

Chen

et al. 2013

Journal of Surgical Research

View full text Add to dashboard Cite

Background γ-Aminobutyric acid exists throughout the body, and the brain γ-aminobutyric acid receptor (GABAR) regulation reduces oxidative stress (OS). Effects of GABAR regulation in the liver are unknown. Ischemia or reperfusion injury after orthotopic liver transplantation (OLT) or shear stress after split OLT (SOLT) with a small-for-size graft causes OS-induced graft damage. Here, the strategic potential of graft pretreatment in vivo and ex vivo by GABAR regulation was investigated. Materials and methods Recipient rats were divided into seven groups according to the graft pretreatments and graft types: (1) laparotomy, (2) OLT, (3) GABAR regulation in vivo and OLT, (4) GABAR regulation ex vivo and OLT, (5) SOLT, (6) GABAR regulation in vivo and SOLT, and (7) GABAR regulation ex vivo and SOLT. Survival study, biochemical assays, histopathologic or immunohistologic assessments, and Western blotting were performed at 6 h after OLT or SOLT. Results Graft pretreatment in vivo prolonged survival after SOLT. Histopathologic and biochemical profiles verified that graft pretreatment in vivo reduced graft damage after OLT or SOLT. Immunohistologically, graft pretreatment in vivo prevented apoptotic inductions after OLT or SOLT. The 4-hydroxynonenal confirmed the OS after OLT or SOLT, and graft pretreatment in vivo improved the OS. Graft pretreatment in vivo decreased ataxia-telangiectasia–mutated kinase and H2AX after OLT or SOLT. Graft pretreatment in vivo increased phosphatidylinositol 3 kinase and Akt after SOLT. In contrast, GABAR regulation ex vivo did not work. Conclusions Graft pretreatment in vivo, not ex vivo, prevented the ischemia or reperfusion injury–mediated OS after OLT or SOLT via the ataxia-telangiectasia–mutated kinase/H2AX pathway and the shear stress–mediated OS after SOLT with small-for-size graft via the phosphatidylinositol 3 kinase/Akt pathway.

show abstract

Matrix Metalloproteinase 2 in Reduced‐Size Liver Transplantation: Beyond the Matrix

Cited by 17 publications

References 66 publications

Oxidative stress and extracellular matrices after hepatectomy and liver transplantation in rats

Oxidative stress and extracellular matrices after hepatectomy and liver transplantation in rats

Hepatic arterial reconstruction for orthotopic liver transplantation in the rat

Liver graft pretreated in vivo or ex vivo by γ-aminobutyric acid receptor regulation

Contact Info

Product

Resources

About