Matrix Metalloproteinase-9 −1562C/T Gene Polymorphism Is Associated with Diabetic Nephropathy

Feng, Shufen; Ye, Gang; Bai, Shi; Wei, Hongcheng; Liao, Xueling; Li, Lü

doi:10.1155/2016/1627143

Cited by 10 publications

(15 citation statements)

References 24 publications

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“…For amplification of the region containing the MMP-9 −1562C/T polymorphism, the following primers were used: sense primer 5-CTT CCT AGC CAG CCG GCA TC-3 and antisense 5-GCC TGG CCT ATA GTA GGC CC-3 [13]. PCR amplification was performed using DreamTaq PCR Green Master Mix (2X) (cat no k1081, Lithuania, EU).…”

Section: Dna Extractionmentioning

confidence: 99%

“…PCR amplification was performed using DreamTaq PCR Green Master Mix (2X) (cat no k1081, Lithuania, EU). PCR was performed using the following thermal cycling conditions [13] Digestion of PCR products with SphI restriction endonuclease Thermo Scientific FastDigest, cat no FD 0604, Lithuania, EU, to detect the MMP-9 −1562C/T SNP [13]. Separation of restriction products by electrophoresis in 2% agarose gels, after that visualization of the restriction products using an ultraviolet transilluminator.…”

Section: Dna Extractionmentioning

confidence: 99%

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Matrix metalloproteinase-9 gene polymorphism (-1562 C/T) and its correlation with diabetic nephropathy

et al. 2021

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Background Matrix metalloproteinase 9 (MMP-9) is an important inflammatory marker in diabetic nephropathy. Many studies assessed the association between MMP-9 gene polymorphism and different microvascular complications of type 2 diabetes mellitus, though the results were inconclusive and need further exploration. Our study aimed to assess the association between MMP-9 -1562C/T gene polymorphism and diabetic nephropathy in patients with type 2 diabetes mellitus. Results Taking CC genotype of rs3918242 (MMP-9-1562C/T SNP) as the reference genotype and C as the reference allele, TT genotype, T allele showed significantly lower frequency in diabetic nephropathy group than without nephropathy (2.9% versus 20%, 20% versus 35.7% respectively), with the possible significant protective effect against diabetic nephropathy development (OR = 0.269, 0.450 respectively); it was considered as an independent predictor for diabetic nephropathy occurrence. Conclusions This study suggested that T allele of MMP-9 -1562C/T single nucleotide polymorphism had a protective role against diabetic nephropathy development and also had a role for early prediction of patients susceptible to this complication, so it helps in prevention and management of those patients.

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Section: Dna Extractionmentioning

confidence: 99%

Section: Dna Extractionmentioning

confidence: 99%

Matrix metalloproteinase-9 gene polymorphism (-1562 C/T) and its correlation with diabetic nephropathy

et al. 2021

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“…Moreover, MMPs, including MMP9 , are zinc-dependent endopeptidases and the major proteases in ECM degradation. There are common variants such as rs3918242 (-1562C/T) and microsatellites (CA)n in the promoter region and several SNPs rs481480, rs2032487, rs4281481, rs3752462 and rs3918242 are found to be associated with the susceptibility to DKD (Hirakawa et al, 2003; Nair et al, 2008; Ahluwalia et al, 2009; Freedman et al, 2011; Cooke et al, 2012; Zhang et al, 2015; Feng et al, 2016).…”

Section: Recent Data From Genetic Studies In Diabetic Kidney Diseasementioning

confidence: 99%

Genetic and Epigenetic Studies in Diabetic Kidney Disease

2019

Front. Genet.

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Chronic kidney disease is a worldwide health crisis, while diabetic kidney disease (DKD) has become the leading cause of end-stage renal disease (ESRD). DKD is a microvascular complication and occurs in 30–40% of diabetes patients. Epidemiological investigations and clinical observations on the familial clustering and heritability in DKD have highlighted an underlying genetic susceptibility. Furthermore, DKD is a progressive and long-term diabetic complication, in which epigenetic effects and environmental factors interact with an individual’s genetic background. In recent years, researchers have undertaken genetic and epigenetic studies of DKD in order to better understand its molecular mechanisms. In this review, clinical material, research approaches and experimental designs that have been used for genetic and epigenetic studies of DKD are described. Current information from genetic and epigenetic studies of DKD and ESRD in patients with diabetes, including the approaches of genome-wide association study (GWAS) or epigenome-wide association study (EWAS) and candidate gene association analyses, are summarized. Further investigation of molecular defects in DKD with new approaches such as next generation sequencing analysis and phenome-wide association study (PheWAS) is also discussed.

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“…A series of epidemiological researches have assessed the association between the MMP-9 gene polymorphism and the risk of DN. [ 25 – 27 ] The most common variants are microsatellites (CA)n [ 28 ] and rs3918242 (-1562C/T) [ 26 , 27 ] promoter polymorphisms. A recent meta-analysis provided potential evidence of the association between MMP-9 gene promoter polymorphism and the risk of diabetic microvascular complications, [ 26 ] but there is no system review specifically evaluating MMP-9 SNPs in DN to date.…”

Section: Introductionmentioning

confidence: 99%

Association of MMP-9 polymorphisms with diabetic nephropathy risk

Xie¹,

Wang²,

Chang³

et al. 2020

Medicine

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Background: Diabetic nephropathy (DN) is a multifactorial disease with gene–environment interaction resulting in progressive renal function damage. Multiple studies have assessed the association between matrix metalloproteinase-9 (MMP-9) gene promoter polymorphism and DN susceptibility. However, the results are inconclusive. In the present study, we will conduct a meta-analysis to further examine this relationship more precisely. Methods: Electronic databases (Pubmed, Web of Science, Embase, Google Scholar, Wanfang, China Biological Medicine and China National Knowledge Infrastructure) will be used to search clinical case–control studies about MMP-9 polymorphism and DN published until 18 August 2020. The language will be restricted to Chinese and English. Two reviewers will take charge of completing the selection of study, the extraction of data as well as the assessment of study quality independently. The Newcastle-Ottawa Scale will be used to evaluate the study quality. We will evaluate the association under 5 genetic models. Fixed-effects or random-effects models will be used to calculate the effect sizes of odds ratio and 95% confidence intervals. Afterwards, subgroup analysis will be conducted in terms of the ethnicity and genotyping method. Additionally, sensitivity analysis will be performed via sequentially omitting each of the included studies one at a time. The funnel plots, Egger regression test, and Begg rank correlation test will be used to test the potential publication bias. All the statistical analyses will be performed using Review Manager 5.3 and Stata 12.0. Results: This protocol reported according to the Preferred Reporting ltems for Systematic Reviews and Meta-Analyses Protocols (PRISMA-P) statement. This study will provide a better understanding of the association between MMP-9 polymorphisms and DN risk. Conclusion: Publishing this protocol will minimize the potential bias related to data mining, thus contributing to generation of reliable evidence. OSF registration number: DOI 10.17605/OSF.IO/H5FS4

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Matrix Metalloproteinase-9 −1562C/T Gene Polymorphism Is Associated with Diabetic Nephropathy

Cited by 10 publications

References 24 publications

Matrix metalloproteinase-9 gene polymorphism (-1562 C/T) and its correlation with diabetic nephropathy

Matrix metalloproteinase-9 gene polymorphism (-1562 C/T) and its correlation with diabetic nephropathy

Genetic and Epigenetic Studies in Diabetic Kidney Disease

Association of MMP-9 polymorphisms with diabetic nephropathy risk

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