Matrix metalloproteinases 2 and 9 and MMP9/NGAL complex activity in women with PCOS

Ranjbaran, Javad; Farimani, Marzie; Tavilani, Heidar; Ghorbani, Marzieh; Karimi, Jamshid; Poormonsefi, Faranak; Khodadadi, Iraj

doi:10.1530/rep-15-0340

Cited by 36 publications

(26 citation statements)

References 34 publications

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“…Secondly, in the present study we quantified free MMP‐9 levels that is both pro‐MMP‐9 and active MMP‐9 levels. However, we have not measured MMP‐9/NGAL complex concentrations, which is believed to have an additive functional affect . Anticoagulants used for collection of blood samples were shown to altered activity of MMP‐9 .…”

Section: Discussionmentioning

confidence: 92%

“…However, we have not measured MMP-9/ NGAL complex concentrations, which is believed to have an additive functional affect. 28 Anticoagulants used for collection of blood samples were shown to altered activity of MMP-9. 29 In the current report, we have used EDTA as anticoagulant and samples collected in EDTA has been shown optimal activity of both pro and mature MMP-9.…”

Section: Discussionmentioning

confidence: 99%

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Role of matrix metalloproteinase‐9 gene polymorphisms in glaucoma: A hospital‐based study in Chinese patients

Zhao

Fan

Huang

2019

Clinical Laboratory Analysis

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Background Glaucoma is the irreversible vision loss and contributes second leading cause of blindness worldwide. Matrix metalloproteinase‐9 (MMP‐9) is involved with remodeling and destruction of extracellular matrix. Elevated MMP‐9 levels and various functional variants of MMP‐9 have been associated with glaucoma in different population. In the current investigation, we tested association of MMP‐9 common variants with different clinical categories of glaucoma in Chinese population. Materials and Methods We enrolled total of 396 glaucoma patients those reported to hospital comprising of 212 primary angle closure glaucoma (PACG) cases and 184 primary open‐angle glaucoma POAG patients. In addition, 329 normal individuals from similar geographical areas were enrolled as healthy controls. Five common single nucleotide polymorphisms (rs3918242, rs3918254, rs2250889, rs3918249, and rs17576) were genotyped by PCR‐RFLP. Plasma levels of MMP‐9 were quantified by ELISA. Results Heterozygotes (GC) and allele “G” for rs2250889 polymorphism were more frequent in PACG cases compared with healthy controls (GC: P < .0001, OR = 2.26; G: P < .0001, OR = 1.19). Similarly, heterozygous mutant and minor allele for rs3918242 polymorphism were more prevalent in POAG in comparison with healthy controls. Interestingly, distribution of rs17576 variant was statistically higher in both PACG and POAG cases than healthy controls. Furthermore, analysis of plasma MMP‐9 with MMP‐9 polymorphisms revealed significant association of rs2250889, rs3918242, and rs17576 with plasma levels of the protein. Conclusions MMP‐9 mutants are associated with elevated plasma MMP‐9 and predisposed to development of glaucoma.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Role of matrix metalloproteinase‐9 gene polymorphisms in glaucoma: A hospital‐based study in Chinese patients

Zhao

Fan

Huang

2019

Clinical Laboratory Analysis

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“…The study of Ranjbaran et al in 2016 was the rst one on the involvement of the activity of MMP-9 in pathogenesis of PCOS. They showed that the serum level of MMP-9 was signi cantly higher in women with PCOS compared to the control group (42). The study of Dambala et al in 2017 also showed the same result (43).…”

Section: Discussionmentioning

confidence: 78%

Assessment of gingival inflammation indexes and matrix metalloproteinase (MMP-9) in patients with polycystic ovarian syndrome compared to control group

Maboudi¹,

Shafizade²,

Amiri³

et al. 2020

Preprint

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Background: There is emerging evidence to support the link between polycystic ovarian syndrome (PCOS) and periodontal disease, but the mechanisms associated with these two diseases have not been clearly elucidated but relate to different aspects of inflammation. This study aimed to determine the gingival inflammation indexes and matrix metalloproteinase-9 (MMP-9) in patients with polycystic ovarian syndrome compared to control group.Methods: This is a case-control study in which 87 women referred to the Babol Clinic Private Hospital were divided into three groups: 26 women with PCOS diagnosed on the basis of Rotterdam criteria with gingivitis, 26 women with PCOS without gingivitis and 26 healthy women who were matched for age and body mass index. After recording the underlying variables, fasting saliva samples were taken from all participants in the morning before any periodontal intervention. The samples, which were transferred to Babol Molecular Cell Research Center under cold-chain conditions to measure the serum levels of MMP-9 using ELISA kits. Periodontal status was evaluated for Gingival index (GI), bleeding on probing (BOP), plaque index (PI). Analysis of variance was used to compare the results for the normal quantitative variables and Chi-square test was used to compare the qualitative variables. Significance level was considered less than 0.05.Results: The highest mean periodontal disease indexes (GI, BOP, PI) were observed in women with PCOS with gingivitis and then in women with PCOS without gingivitis and then in healthy women in control group (p <0.05). The mean salivary MMP-9 levels were significantly higher in PCOS women (388/37 ± 75.05) compared to the control group (166/25 ± 35/43). Even in PCOS patients with healthy gingiva (233.00± 47.76) had higher levels of salivary MMP-9 than the control group and this difference was significant (p <0.05)Conclusions: There is a positive association between periodontal disease and PCOS and that salivary MMP-9 levels are higher in PCOS women with and without gingivitis than healthy women. However, it is recommended that multicenter study with larger sample sizes are to be conducted to establish a clearer association.

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“…Tokmak et al, also reported the similar results [16] . While Ranjbaran et al, reported that a noticeable decline in FSH and an increase in LH levels together with a significant increase in LH/FSH ratio were found in PCOS patients [19] .…”

Section: Discussionmentioning

confidence: 94%

Role Of Insulin Like Growth Factor in Polycystic Ovary Syndrome

El-Hassanin

Shemais²,

and³

2018

The Egyptian Journal of Hospital Medicine

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Objectives:The polycystic ovary syndrome (PCOS) is conventionally defined as a combination of hyperandrogenism and anovulation with polycystic ovaries. Insulin-like growth factor I (IGF-I) is a 70 aa polypeptide hormone with endocrine, paracrine, and autocrine effects. it is now established that the ovary is a site of IGF-I gene expression and reception. However, this huge amount of data could be somehow distractive since different species may produce distinct IGFs (or their relative binding proteins and receptors) at different stages of follicular development. Objective: The aim of this work was to determine the serum level of IGF-I to evaluate its role in PCOS. Subjects and Methods: Twenty-five infertile women with PCOS diagnosed by ultrasound examination and a history of oligomenorrhea, hirsutism and obesity were studied. Serum levels of insulin-like growth factor-1 (IGF-1) and hormonal profile were measured. Fifteen healthy and fertile women with regular menstrual cycles served as a comparison group. Results: The serum levels of IGF-I were significantly elevated in women with PCOS compared with control and there was positive correlation between IGF-I from one side and each of BMI, W/H ratio, LH, LH/FSH ratio, TSN, T/SHBG ratio, right and left ovarian volumes. On the other side, IGF-I was inversely correlated with SHBG, right and left uterine artery PI and RI. Conclusion: The significant finding of this study is that PCOS patients have increased circulating IGF-I levels than healthy controls and it can be used as a biochemical marker for PCOS.

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Matrix metalloproteinases 2 and 9 and MMP9/NGAL complex activity in women with PCOS

Cited by 36 publications

References 34 publications

Role of matrix metalloproteinase‐9 gene polymorphisms in glaucoma: A hospital‐based study in Chinese patients

Role of matrix metalloproteinase‐9 gene polymorphisms in glaucoma: A hospital‐based study in Chinese patients

Assessment of gingival inflammation indexes and matrix metalloproteinase (MMP-9) in patients with polycystic ovarian syndrome compared to control group

Role Of Insulin Like Growth Factor in Polycystic Ovary Syndrome

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