Matrix Metalloproteinases 2 and 9 and E-Cadherin Expression in the Endometrium During the Implantation Window of Infertile Women Before In Vitro Fertilization Treatment

Abstract

Objective: To evaluate the expression of endometrial matrix metalloproteinases (MMPs) 2 and 9 and E-cadherin in periimplantation phase of infertile women who have undergone in vitro fertilization (IVF) cycles. Methods: This prospective study included 51 patients who underwent endometrial biopsy during the receptive phase in a menstrual cycle prior to IVF treatment. The samples were evaluated by tissue microarray for immunohistochemical study. Results: The expression of MMP-2, MMP-9, and E-cadherin in the endometrium prior to IVF treatment was not associated with pregnancy. There was a decrease in E-cadherin immunodetection, the higher the age of the patients, a negative relationship between E-cadherin and MMP-2, and a positive association between MMP-9 and E-cadherin. Conclusions: The MMP-2, MMP-9, and E-cadherin are expressed in the endometrium of infertile patients during the receptive phase of the natural menstrual cycle. However, there is no correlation between the expression of these molecules and the clinical IVF outcomes.

Factors related to infertility in Brazil and their relationship with success rates after assisted reproduction treatment: an integrative review

“…Through the categorized analysis of the 27 articles that composed the final sample, we found that all were published between 2003 and 2019. Of these, 4 were cross-sectional studies ( Pasqualotto et al ., 2003 ; Esteves & Glina, 2005 ; Cota et al ., 2012 ; Souza et al ., 2017 ), 12 were cohort studies ( Pinheiro et al ., 2003 ; Borges et al ., 2003 ; Glina et al ., 2005 ; Pasqualotto et al ., 2005 ; Romão et al ., 2010 ; Semião-Francisco et al ., 2010 ; Cota et al ., 2012 ; Borges et al ., 2013 ; Coelho Neto et al ., 2015 ; Maia-Filho et al ., 2015 ; Scheffer et al ., 2017 ; Bercaire et al ., 2018 ); 6 were case-control studies ( Setti et al ., 2011 ; Pasqualotto et al ., 2012 ; Cavagna et al ., 2012 ; Picinato et al ., 2014 ; Donabela et al ., 2015 ; Costa et al ., 2016 ); 3 were case reports ( Valle et al ., 2012 ; Taitson et al ., 2012 ; Borges et al ., 2016 ) and 2 were randomized controlled trials ( Geber & Sampaio, 2013 ; Nastri et al ., 2013 ).…”

“…In 15.21% of the articles included in this review, endometriosis is cited as the main condition causing female infertility ( Cota et al ., 2012 ; Nastri et al ., 2013 ; Maia-Filho et al ., 2015 ; Picinato et al ., 2014 ; Donabela et al ., 2015 ; Coelho Neto et al ., 2015 ; 2016 ); and in 13.04% azoospermia is the male infertility condition ( Pasqualotto et al ., 2003 ; Glina et al ., 2005 ; Esteves & Glina, 2005 ; Pasqualotto et al ., 2005 ; Semião-Francisco et al ., 2010 ; Taitson et al ., 2012 ). Figure 2 shows the other infertility conditions presented by the patients.…”

“…Regarding the assisted reproduction techniques, 7 studies (25.92%) mentioned that patients were submitted to intracytoplasmic sperm injection (ICSI) or conventional in vitro fertilization (IVF) ( Cota et al ., 2012 ; Geber & Sampaio, 2013 ; Coelho Neto et al ., 2015 ; 2016 ; Costa et al ., 2016 ; Souza et al ., 2017 ; Bercaire et al ., 2018 ); 18 studies (66.66%) cited ICSI exclusively ( Pinheiro et al ., 2003 ; Borges et al ., 2003 ; Pasqualotto et al ., 2003 ; Glina et al ., 2005 ; Esteves & Glina, 2005 ; Pasqualotto et al ., 2005 ; Romão et al ., 2010 ; Semião-Francisco et al ., 2010 ; Setti et al ., 2011 ; Pasqualotto, et al ., 2012 ; Valle et al ., 2012 ; Taitson et al ., 2012 ; Nastri et al ., 2013 ; Picinato et al ., 2014 ; Maia-Filho et al ., 2015 ; Donabela et al ., 2015 ; Borges et al ., 2016 ; Scheffer et al ., 2017 ); and 2 studies (7.40%) cited the Intracytoplasmic Injection of Morphologically Selected Sperm (IMSI) ( Cavagna et al ., 2012 ; Borges et al ., 2013 ). IVF was used mainly in cases associated with female infertility, such as endometriosis, poor ovarian response, polycystic ovary syndrome and advanced age.…”

Dysregulation of collagen expression in peri‐implantation endometrium of women with high ovarian response

“…In mid‐secretory endometria of patients with unexplained infertility and recurrent miscarriages, one study reported higher levels of MMP2 and decreased levels of TIMP3 mRNA, but another found that MMP2 and TIMP3 expressions were significantly decreased simultaneously . However, other studies reported that MMP2, MMP9 and TIMP1 expressions in endometrial samples revealed no significant differences between idiopathic infertility, unexplained recurrent miscarriage and control group, and were not associated with IVF pregnancy outcome . This may be related to different sources of the endometrium sample.…”

Fractalkine Regulates HEC-1A/JEG-3 Interaction by Influencing the Expression of Implantation-Related Genes in an In Vitro Co-Culture Model

“…MMPs play a critical role in the invasion of trophoblast cells. Among MMPs, MMP2 and 9 seem to be essential in the regulation of invasion and the behaviours of trophoblasts [62][63][64]. During the implantation, trophoblast cells release large amounts of MMP2 and 9 [65].…”