Matrix metalloproteinases and E-cadherin immunoreactivity in different basal cell carcinoma histological types

Vanjaka-Rogošić, Lucija; Puizina-Ivić, Neira; Mirić, Lina; Rogošić, Veljko; Kuzmić-Prusac, Ivana; Babić, Maja; Vuković, Dubravka; Mardesic, Snjezana

doi:10.1016/j.acthis.2013.12.007

Cited by 16 publications

(19 citation statements)

References 24 publications

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“…A number of previous studies showed an increase in the expressions of MMP-2 and MMP-9 within various malignancies, and these enzymes were proposed as potential cancer markers [ 25 , 44 , 49 – 52 ]. Our findings are consistent with the data published by Vempati et al [ 46 ], Zlatarova et al [ 25 ], Fu et al [ 50 ], El-Khalawany and Abou-Bakr [ 24 ] and Vanjaka-Rogosic et al [ 53 ], who showed that the expression of MMP-9 may constitute a molecular marker of processes taking place within the BCCs. However, the evidence in this matter is inconclusive.…”

Section: Discussionmentioning

confidence: 99%

Expression of metalloproteinases (MMP-2 and MMP-9) in basal-cell carcinoma

et al. 2016

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The aim of this study was to compare the expressions of mRNA for metalloproteinases (MMP-2 and MMP-9) and type IV collagen in two different histological types of basal-cell carcinoma (BCCs; nodular and infiltrative) and in normal tissues from the tumor interface. The study included biopsy specimens of the skin involved with BCC and normal skin adjacent the lesion. The expressions of mRNA for MMP-2, MMP-9 and type IV collagen were determined by means of RT-PCR (Reverse transcription polymerase chain reaction). The level of type IV collagen mRNA in nodular and infiltrative BCCs turned out to be significantly lower, and the expressions of MMP-2 and MMP-9 mRNA significantly higher than in normal tissues adjacent to these tumors. The expression of mRNA for MMP-9 but not for MMP-2 was significantly higher in infiltrative BCCs than in the nodular BCCs. In turn, normal tissues adjacent to nodular BCCs showed significantly higher levels of mRNA for MMP-2 and significantly lower levels of type IV collagen mRNA than the normal tissues from the interface of infiltrative BCCs. The findings suggest that MMP-2 and MMP-9 could be used as prognostic factors of BCCs.

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Section: Discussionmentioning

confidence: 99%

Expression of metalloproteinases (MMP-2 and MMP-9) in basal-cell carcinoma

et al. 2016

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“…Tissue pieces were fixed in 4% paraformaldehyde in phosphate buffer and dehydrated in 100% ethanol. They were paraffin embedded and processed as we described previously 21,22 . Haematoxylin and Eosin staining was used on every 10 th section to confirm appropriate tissue preservation.…”

Section: Methodsmentioning

confidence: 99%

Expression of apoptotic and proliferation factors in gastric mucosa of patients with systemic sclerosis correlates with form of the disease

Borić

Mardesic

Kaliterna

et al. 2019

Sci Rep

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Despite high prevalence of patients with gastric disease in systemic sclerosis (SSc), its pathogenesis is still poorly understood. We immunohistochemically analysed biopsies of gastric mucosa (GM) in 5 controls and 15 patients with different forms of SSc: limited cutaneous (lc), diffuse cutaneous moderate (sys1) and severe (sys2). The number of positive cells was analysed by a Kruskall-Wallis test, P < 0.05 was considered statistically significant. Percentage of proliferating (Ki-67 positive) cells was highest in sys1 (3% in superficial and 4,6% in deeper parts of GM), which dropped to 1% in sys2. Percentage of α-smooth muscle actin (α-SMA) positive cells was 5% in controls, 9% in superficial GM, while in deeper GM rose from 7% to 19% in sys1 and sys2, thus indicating increased myofibroblast population. Caspase-3 positive apoptotic cells characterized 1,5–2% of controls, 8% of superficial and 6% of deeper GM cells in sys1. In sys2, apoptosis affected 50% of surface epithelial and gland cells and 30% of deeper glands, and correlated with increased fibrosis and decreased syndecan-1 expression. Our data demonstrate that sys1 is the most „active” proliferating form of SSc. Sys2 characterize collagen deposition, surface epithelium defects, extensive apoptosis and low proliferation, GM atrophy and loss of function.

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“…MMP-9 prominently expressed at the invading edge of the BCC and was mostly secreted by inflammatory cells, such as macrophages, rather than by tumour cells [158,168]. Odds ratio for development of morpheaform and recurrent BCC was 5.8 for positive MMP-9 immunostaining in tumour stroma, suggesting MMP-9 expression in stromal cells is associated with morpheaform and recurrent BCC [161]. In order to compare the expressions of mRNA for metalloproteinases (MMP-2 and MMP-9) and type IV collagen in BCC and normal tissues from the tumour interface, Goździalska et al detected the expressions of mRNA for MMP-2, MMP-9 and type IV collagen by RT-PCR.…”

Section: Gelatinasesmentioning

confidence: 94%

“…The results showed that the loss of palisading arrangement in BCC was correlated with the MMP-1 expression of stromal cells [160]. Odds ratio for development of morpheaform and recurrent BCC was 6.2 for positive MMP-1 immunostaining in epithelial tumour cells, suggesting MMP-1 is associated with morpheaform and recurrent BCC [161]. MMP-13 is involved in the degradation of ECM and its expression is associated with malignant transformation in skin carcinogenesis [155,162].…”

Section: Collagenasesmentioning

confidence: 99%

Skin Ageing and Cancer

Zhang¹,

Wang²,

Wang³

2017

The Role of Matrix Metalloproteinase in Human Body Pathologies

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Human matrix metalloproteinases (MMPs) belong to the M10 family of the MA clan of endopeptidases. They are ubiquitarian enzymes, structurally characterized by an active site where a Zn 2+ atom, coordinated by three histidines, plays the catalytic role, assisted by a glutamic acid as a general base. Based on their structure and substrate specificity, they can be categorized into five main subgroups, namely (1) collagenases (MMP-1, MMP-8 and MMP-13); (2) gelatinases (MMP-2 and MMP-9); (3) stromelysins (MMP-3, MMP-10 and MMP-11); (4) matrilysins (MMP-7 and MMP-26) and (5) membrane-type (MT) MMPs (MMP-14, MMP-15, MMP-16, MMP-17, MMP-24 and MMP-25). MMPs can act on extracellular matrix (ECM) and non-ECM components affecting degradation and modulation of the ECM, growth-factor activation and cell-cell and cell-matrix signalling. In skin, MMPs are secreted by different cell types such as fibroblasts, keratinocytes, macrophages, endothelial cells, mast cells, and eosinophils. This chapter reviews the role of MMPs in maintaining skin homeostasis, skin ageing and skin cancer.

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Matrix metalloproteinases and E-cadherin immunoreactivity in different basal cell carcinoma histological types

Cited by 16 publications

References 24 publications

Expression of metalloproteinases (MMP-2 and MMP-9) in basal-cell carcinoma

Expression of metalloproteinases (MMP-2 and MMP-9) in basal-cell carcinoma

Expression of apoptotic and proliferation factors in gastric mucosa of patients with systemic sclerosis correlates with form of the disease

Skin Ageing and Cancer

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