Maturation-associated immunophenotypic abnormalities in bone marrow B-lymphocytes in myelodysplastic syndromes

Ribeiro, Evandro Marcos Saidel; Matarraz, Sergio; Santiago, M. de Jesús Gallegos; Lima, Carmen S. P.; Metze, Konradin; Giralt, Manuel; Saad, Sara Teresinha Olalla; Matos, Alberto Orfao de; Lorand-Metze, Irene

doi:10.1016/j.leukres.2005.05.019

Cited by 33 publications

(38 citation statements)

References 30 publications

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“…Such increased number of CD34 þ cells was mainly due to a progressive expansion of immature precursors found to be abnormally increased in most high-grade MDS cases. In line with previous observations from our 35 and other groups, 36 the increase of immature precursors was associated with decreased numbers of BCP and, to a lower extent, also of neutrophil, plasmacytoid DC, basophil and erythroid-committed CD34 þ HPC, except among low-grade MDS cases, where a shift toward the production of neutrophil and erythroid lineage cells, at the expense of B-cell and plasmacytoid DC precursors, was observed in a significant proportion of cases.…”

Section: Distribution Of Total Bm Cd34supporting

confidence: 94%

“…In line with previous observations, 35 BCP were not only progressively decreased in number from low-to high-grade MDS, but they also showed a pronounced aberrant phenotype characterized by abnormally decreased expression of CD79a, associated with decreased TdT and HLA-DR expression among INT-1 and LOW-R MDS cases, respectively. Altogether, these results indicate that a progressively decreased and abnormal production of B-lymphocytes might occur in MDS from low-to high-grade cases, as also suggested by others.…”

Section: Cd34supporting

confidence: 92%

“…Altogether, these results indicate that a progressively decreased and abnormal production of B-lymphocytes might occur in MDS from low-to high-grade cases, as also suggested by others. 35,36,42 Whether B cells in MDS are also derived from the neoplastic clone or these abnormalities just reflect a shift toward the production of myeloid cells essential to patient survival remains a matter of debate and requires further investigations.…”

Section: Cd34mentioning

confidence: 99%

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The immunophenotype of different immature, myeloid and B-cell lineage-committed CD34+ hematopoietic cells allows discrimination between normal/reactive and myelodysplastic syndrome precursors

et al. 2008

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Occurrence of phenotypic abnormalities in CD34þ hematopoietic progenitor and precursor cells (HPC) and their major B-cell and nonlymphoid compartments has been frequently reported in myelodysplastic syndromes (MDS). Here, we analyze for the first time the numerical and phenotypic abnormalities of different maturation-associated subsets of bone marrow (BM) CD34þ HPC from 50 newly diagnosed MDS patients in comparison to normal/reactive BM (n ¼ 29). Our results confirm the existence of heterogeneously altered phenotypes among CD34 þ HPC from MDS and indicate that such variability depends both on the relative distribution of the different subsets of CD34 þ HPC committed into the different myeloid and B-lymphoid compartments, and their immunophenotype (for example, higher reactivity for CD117 and CD13 and lower expression of CyMPO, CD64 and CD65 on CD34 þ immature and neutrophil precursors), a clear association existing between the accumulation of CD34 þ HPC and that of immature CD34 þ HPC. Interestingly, expansion of erythroidand neutrophil-lineage CD34 þ cells is detected in low-grade MDS at the expense of CD34 þ plasmacytoid dendritic cell and B-cell precursors, while expansion of immature CD34 þ precursors occurs in high-grade MDS. On the basis of the number and severity of the phenotypic abnormalities detected, a scoring system is proposed that efficiently discriminates between normal/reactive and MDS CD34 þ HPC, the mean score significantly increasing from low-to high-grade MDS.

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Section: Distribution Of Total Bm Cd34supporting

confidence: 94%

Section: Cd34supporting

confidence: 92%

Section: Cd34mentioning

confidence: 99%

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The immunophenotype of different immature, myeloid and B-cell lineage-committed CD34+ hematopoietic cells allows discrimination between normal/reactive and myelodysplastic syndrome precursors

et al. 2008

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“…Perhaps the most common abnormality that we, 6 and others 9,11,12,14,15 have documented are reduced numbers of B-cell CD34 + progenitors in MDS. As B-cell progenitors have higher CD38 expression, and thus a higher CD38 relative mean fluorescent intensity (RFMI) as a population, we hypothesized that a lower number of B-cell progenitors would reduce the mean fluorescence intensity (MFI) of CD38 expression on CD34 + cells.…”

Section: Introductionmentioning

confidence: 75%

Reduced CD38 expression on CD34+ cells as a diagnostic test in myelodysplastic syndromes

Goardon¹,

Nikolousis²,

Sternberg³

et al. 2009

Haematologica

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“…A decrease of CD34+ B-cell precursors has also been repeatedly described [4,11,12]. Recently, Satoh et al [4] stated that the proportion of CD34+/CD19+ B-cell precursors among all CD34+ bone marrow (BM) cells is an easily reproducible parameter for the differential diagnosis between low-grade MDS and non-clonal disorders presenting peripheral cytopenias.…”

Section: Methodsmentioning

confidence: 96%

Variation of Bone Marrow Cd34+ Cell Subsets in Myelodysplastic Syndromes According to Who Types

Reis¹,

Traina²,

Metze³

2009

neo

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Bone marrow (BM) hematopoietic progenitor cells (CD34+) are a heterogeneous population with varying degrees of commitment and maturation to several cell lineages. In myelodysplastic syndromes (MDS), this population is increased. We examined the major cell types found in the blast gate by flow cytometry in newly diagnosed patients with MDS, compared them to normal BM and studied their variation according to WHO type. Two subsets defined by SSC were found both in normal BM and MDS, corresponding to myeloblasts and B-cell precursors. The number of B-cell precursors among all nucleated cells was equally low, independent of WHO type. However, the subset with an intermediate SSC, but CD117, CD13 and CD19 negative increased with the rise of myeloblasts. Concomitantly, the ratio between CD34+/CD117+/CD34-/CD117+ cells was increased. These two features are consistent with the maturation block occurring in the progression of the neoplastic clone. We conclude that the quantitative analysis of the cell types present in the BM blast gate by flow cytometry is not only important for the diagnosis of MDS in patients with peripheral cytopenias and a normal karyotype, but gives also important prognostic information of the patients

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Maturation-associated immunophenotypic abnormalities in bone marrow B-lymphocytes in myelodysplastic syndromes

Cited by 33 publications

References 30 publications

The immunophenotype of different immature, myeloid and B-cell lineage-committed CD34+ hematopoietic cells allows discrimination between normal/reactive and myelodysplastic syndrome precursors

The immunophenotype of different immature, myeloid and B-cell lineage-committed CD34+ hematopoietic cells allows discrimination between normal/reactive and myelodysplastic syndrome precursors

Reduced CD38 expression on CD34+ cells as a diagnostic test in myelodysplastic syndromes

Variation of Bone Marrow Cd34+ Cell Subsets in Myelodysplastic Syndromes According to Who Types

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