Mature fruit abscission is associated with up-regulation of polyamine metabolism in the olive abscission zone

Gómez-Jiménez, M.C.; Paredes, Miguel A.; Gallardo, Mercedes; Sánchez‐Calle, Isabel María

doi:10.1016/j.jplph.2010.05.020

Cited by 35 publications

(44 citation statements)

References 43 publications

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“…Although up to 90% of the PA pool is in the conjugated form in olive flower and fruit (Gomez-Jimenez et al 2010b), conjugates represented 40-50% of the whole Put pool in ET-treated olive AZ-AC. A relative proportion of the soluble-and insoluble-conjugated PA in fruit AZ-AC is diversified among the two cultivars and applied treatments.…”

Section: Pa Metabolism During Et-induced Mature-fruit Abscissionmentioning

confidence: 95%

“…Ornithine decarboxylase, arginine decarboxylase, and S-adenosylmethionine decarboxylase enzyme activities Ornithine decarboxylase (ODC; EC 4.1.1.17), arginine decarboxylase (ADC; EC 4.1.1.19), and S-adenosylmethionine decarboxylase (SAMDC; EC 4.1.1.50) were determined by a radiochemical method as described by Gomez-Jimenez et al (2010b).…”

Section: Plant Materials and Treatmentsmentioning

confidence: 99%

“…Assays of diamine (DAO; EC 1.4.3.6) and polyamine oxidase (PAO; EC 1.5.3.3) activities were determined by measuring the generation of H 2 O 2 , a product of the oxidation of PAs, as described by Gomez-Jimenez et al (2010b).…”

Section: Dao and Pao Enzyme Activitiesmentioning

confidence: 99%

“…S1, S2). Therefore, it can be inferred that ADC is a major pathway for Put biosynthesis in fruit AZ-AC, although the ODC pathway has been reported to be responsible of developmental acquisition of cell size during early fruit development (Gomez-Jimenez et al 2010b). Given that the ET and PA biosynthetic pathways are linked through SAM, it appears possible that cobalt ions, which inhibit ACO activity, may raise PA levels by channeling SAM toward the PA biosynthetic pathway.…”

Section: Pa Metabolism During Et-induced Mature-fruit Abscissionmentioning

confidence: 99%

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Regulation of polyamine metabolism and biosynthetic gene expression during olive mature-fruit abscission

Gil-Amado

Gómez-Jiménez

2011

Planta

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Exogenous ethylene and some inhibitors of polyamine biosynthesis can induce mature-fruit abscission in olive, which could be associated with decreased nitric oxide production as a signaling molecule. Whether H₂O₂ also plays a signaling role in mature-fruit abscission is unknown. The possible involvement of H₂O₂ and polyamine in ethylene-induced mature-fruit abscission was examined in the abscission zone and adjacent cells of two olive cultivars. Endogenous H₂O₂ showed an increase in the abscission zone during mature-fruit abscission, suggesting that accumulated H₂O₂ may participate in abscission signaling. On the other hand, we followed the expression of two genes involved in the polyamine biosynthesis pathway during mature-fruit abscission and in response to ethylene or inhibitors of ethylene and polyamine. OeSAMDC1 and OeSPDS1 were expressed differentially within and between the abscission zones of the two cultivars. OeSAMDC1 showed slightly lower expression in association with mature-fruit abscission. Furthermore, our data show that exogenous ethylene or inhibitors of polyamine encourage the free putrescine pool and decrease the soluble-conjugated spermidine, spermine, homospermidine, and cadaverine in the olive abscission zone, while ethylene inhibition by CoCl₂ increases these soluble conjugates, but does not affect free putrescine. Although the impact of these treatments on polyamine metabolism depends on the cultivar, the results confirm that the mature-fruit abscission may be accompanied by an inhibition of S-adenosyl methionine decarboxylase activity, and the promotion of putrescine synthesis in olive abscission zone, suggesting that endogenous putrescine may play a complementary role to ethylene in the normal course of mature-fruit abscission.

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Section: Pa Metabolism During Et-induced Mature-fruit Abscissionmentioning

confidence: 95%

Section: Plant Materials and Treatmentsmentioning

confidence: 99%

Section: Dao and Pao Enzyme Activitiesmentioning

confidence: 99%

Section: Pa Metabolism During Et-induced Mature-fruit Abscissionmentioning

confidence: 99%

See 2 more Smart Citations

Regulation of polyamine metabolism and biosynthetic gene expression during olive mature-fruit abscission

Gil-Amado

Gómez-Jiménez

2011

Planta

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“…Simultaneously, Glu was degraded into nitrogen, carbon skeleton, and energy to support the formation and development of new organs. Polyamines are produced from amino acids (especially arginine) (Gomez-Jimenez et al, 2010) and converted by polyamine oxidases (including DAO) into GABA via a γ-aminobutyl aldehyde intermediate. Xing et al (2007) also found that polyamine content in soybean root increased significantly after recovery from NaCl stress.…”

Section: Discussionmentioning

confidence: 99%

Hypoxia treatment on germinating faba bean (Vicia faba L.) seeds enhances GABA-related protection against salt stress

Yang

Wang

Yin

et al. 2015

Chilean J. Agric. Res.

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The γ-aminobutyric acid (GABA) is a non-protein amino acid with some functional properties for human health. Its content is usually lower in plant seeds. Hypoxia or salt (NaCl) stress is an effective way for accumulating GABA during seed germination. However, NaCl stress on GABA accumulation under hypoxia is currently infrequent. The effect of NaCl on GABA accumulation in germinating faba bean (Vicia faba L.) under hypoxia was therefore investigated in this study. Faba bean seeds were steeped in citric acid buffer (pH 3.5) containing NaCl with a final O2 concentration of 5.5 mg L -1 and germinated for 5 d. Results showed that 60 mmol L -1 NaCl was the optimum concentration for GABA accumulation in germinating faba beans under hypoxia. Germination for 5 d under hypoxia-NaCl stress was less beneficial for GABA accumulation than only hypoxia (control). Polyamine degradation pathway played a more important role for accumulating GABA in germinating faba bean as an adaptive response to NaCl stress. Removing NaCl significantly increased GABA content, while it decreased glutamate decarboxylase (GAD) activity. Simultaneously, polyamine was accumulated, which might be related to the enhancement of physiological activity after recovery. When treated with aminoguanidine (AG) for 3 d, GABA content decreased by 29.82%. These results indicated that the tolerance ability of GABA shunt to NaCl stress was weaker than that of polyamine degradation pathway. The NaCl treatment for 3 d under hypoxia could raise the contribution ratio of polyamine degradation pathway for GABA accumulation. The contribution ratio of polyamine degradation pathway for GABA formation was 29.82% when treated for at least 3 d.

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Diversity and Functional Dynamics of Fleshy Fruit Abscission Zones

Tranbarger

Tadeo

2020

Annual Plant Reviews Online

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We examine the current knowledge about the abscission of fleshy fruit, in particular with regards to the development and function of the abscission zone (AZ). While tomato is currently the main model species to elucidate the mechanisms of fleshy fruit abscission, most studies on tomato focus on the development and function of the pedicel AZ, which is involved in both flower and ripe fruit abscission. However, the localization of the AZ within the pedicel is not the most common location of the AZ for fleshy fruit. Fleshy fruit can have one or more AZ, which can be found in different locations within the inflorescence and fruiting structure and fulfil different functions. In fact, most of the fleshy fruit species examined thus far have more than one AZ, which can be found at the boundary between the fruit and pedicel (often referred to as the calyx AZ), within the pedicel as with tomato, at the juncture of the pedicel and peduncle or at the juncture between peduncle and shoot/branch. In addition, the different AZs may be activated or function at different stages of development, under different environmental conditions, or have a completely different purpose, such as the abscission of fruitlets or mature fruit. Together, our current knowledge about AZs suggests that while there are similarities between different AZs in different locations or from different species, not all AZs are created equal, which may be reflected in the underlying molecular mechanisms that control their development and function.

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Mature fruit abscission is associated with up-regulation of polyamine metabolism in the olive abscission zone

Cited by 35 publications

References 43 publications

Regulation of polyamine metabolism and biosynthetic gene expression during olive mature-fruit abscission

Regulation of polyamine metabolism and biosynthetic gene expression during olive mature-fruit abscission

Hypoxia treatment on germinating faba bean (Vicia faba L.) seeds enhances GABA-related protection against salt stress

Diversity and Functional Dynamics of Fleshy Fruit Abscission Zones

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