Nuclear medicine is a medical science that utilizes radionuclide with gamma ray radiation energy to ionizing the patient’s object either aimed for diagnostic imaging or cancer therapy. Total number of nuclear medicine facilities in Indonesia is 17 hospitals. Unfortunately, there are only 10 hospitals that actively carrying out nuclear medicine services. In order to support the Indonesia cancer management commitment program, the Ministry of Health urges the nuclear medicine facility in Indonesia need to be improved both the quality and facility’s requirement. The standard of nuclear medicine facility services in Indonesia is regulated by the Ministry of Health decree number 008/ MENKES/SK/I/2009, yet unfortunately it has not regulated any specified facility’s technical requirements including layout design, mechanical and electrical aspects. Hence, in this research, we conducted a literature review on the nuclear medicine service facilities’ requirements, referring to the Ministry of Health, the Indonesia Nuclear Energy Agency, and the International Health Facility Guidelines. We conducted thoroughly field observations in ten nuclear medicine facilities in Indonesia to propose the technical requirements and room’s layout design recommendations for nuclear medicine facility in Indonesia. In conclusion of this investigation, the availability of room, utility and instrumentation of nuclear medicine in Indonesia mostly meet the International guidelines, except the room construction aspect. Therefore, our study will promote this shortcoming to fulfill the facility’s requirement recommendation in new regulation of nuclear medicine facilities in Indonesia in the near future for the sake of better patients’ and healthworkers’ safety.