MC3T3-E1 Cell Assay on Collagen or Fibronectin Immobilized Poly (Lactic Acid-ε-Caprolactone) Film

Abstract

In the present study, collagen (Coll) or fibronectin (FN) was immobilized onto poly(lactic acid)/ -caprolactone (PLCL) copolymer surface and the attachment and proliferation of osteoblast-like cells on Coll or FN immobilized PLCL (Coll-PLCL, FN-PLCL) were evaluated. Coll or FN immobilized PLCL was prepared through a condensation reaction between the carboxylic acid groups on the hydrolyzed PLCL (PLCL-COOH) surface and the amino groups of Coll or FN using water soluble carbodiimide. The contact angle of the PLCL-COOH, Coll-PLCL and FN-PLCL surfaces with respect to double distilled water significantly decreased compared with PLCL (p<0.05). Number of attached cells onto Coll-PLCL or FN-PLCL films was significantly greater than on PLCL films from at the time of 90 min to 7days (p<0.05). The morphological differences of attached osteoblast-like cells were observed between PLCL films and Coll-PLCL or FN-PLCL films at the early stage of cell assay. The cell attached on Coll-PLCL and FN-PLCL films appeared more flat than on PLCL films. Additionally, many actin filaments and stress fibers were observed on Coll-PLCL or FN-PLCL films during 3 and 7 days of cell assay. Actin filaments and stress fibers were few on PLCL films. These data demonstrated the effectiveness of collagen and fibronectin immobilization of PLCL for cell attachment and proliferation. This indicates that collagen or fibronectin immobilization of PLCL films was effective for biological activities of osteoblast-like cells.

Effects of Adhesive or Non-adhesive Protein-immobilized Bioactive（Lactic Acid-ε-caprolactone）Films on Mouse Osteoblast-like Cells

“…There were no significant differences in the adhesion cells among the three different PLCL50 films after 7 days (p > 0.05) . The results were consistent with previous studies after 90 min (23) . Even if the proportion of PLA was decreased in the film, the effects of cell adhesionpromoting and cell adhesioninhibiting were confirmed after three days.…”

“…Ho et al (20) reported that many adhesive proteins' RGDS (Arg -Gly -Asp -Ser) peptide was immobi- films. As a result, Alb immobilized -PLCL25 films did not enhance the number and spread of the cells like FNimmobilized PLCL25 films (23) . Alb is a main plasma protein known as a cell adhesion inhibitory protein in contrast to FN and collagen (24,25) .…”

“…However, these barrier membranes have a substandard ability to withstand gingival pressure and maintain the regeneration space (9) . In addition, the materials exhibit no biological activity, such as cell adhesion, proliferation, differentiation, and inhibition (10) .…”

Study of Bioactive Film: Fabrication of Poly (lactic acid) Film Immobilized the Tilapia Fish Collagen or Porcine Collagen and Behavior of MC3T3-E1 Cells

“…Additionally, the collagen contact angle to PLCL film was approximately 56° in previous studies (19) .…”

“…collagen and the carboxylic acid groups on the hydrolyzed PLA using watersoluble carbodiimide. In our previous study, we reported that the number of attached MC3T3 -E1 cells to collagen -PLCL or FN -PLCL significantly accelerated compared with PLCL at 90 min and 3 and 7 days (19) . The number of MC3T3 -E1cells attached to the PSColl -PLA and TFColl -PLA films increased compared with the PLA film during 90 min, 3 and 7 days cell assay (p < 0.05) .…”