MCP-1 and MIP-1A Gene Polymorphisms in Japanese Patients with Sarcoidosis.

Abstract

Objectives Monocyte chemoattractant protein (MCP)-1 and macrophage inflammatory protein (MIP)-loc exhibit chemotactic activity toward macrophages/monocytes and induce the production of inflammatory cytokines affecting granuloma formation. Recently, a single nucleotide polymorphism (SNP) in the MCP-1 distal regulatory region and a dinucleotide repeat in the MIP-1Agene promoter region were identified. Weinvestigated the relationships between the polymorphisms and susceptibility to sarcoidosis, clinical manifestations, and BALFfindings of sarcoidosis. Methods The polymorphisms of the MCP-1and MIP-1A genes in 118 patients with sarcoidosis and 145 healthy control subjects were examined. The MCP-1 polymorphism was genotyped by a PCR-restriction fragment length polymorphism method and the MIP-1Agenotype was determined using PCR. Results No significant difference in the genotype distribution or in the allele frequency between the patients and control subjects was observed. Wefound no relationship between the polymorphisms and the serum ACElevel, organ involvement, roentgenographic stages, or deterioration in chest radiographs during the follow-up. A significant difference in the absolute counts ofAMsin BALFof 51 patients amongthe genotypes of the MCP-1gene was found (p=0.048). The AMcounts in BALF of the G/A and G/G genotypes were significantly increased compared with that of the A/A genotype (p<0.05). Conclusion The polymorphisms of the MCP-1and MIP1Agenes do not play a substantial role in genetic predisposition for sarcoidosis or in clinical manifestations of sarcoidosis in this Japanese population. The MCP-1 SNP might be related to the recruitment of monocytes/macrophages to the alveolar spaces in sarcoidosis. (Internal Medicine 41: 813-818, 2002)

Association analysis between the −2518MCP‐1(A/G) polymorphism and generalized aggressive periodontitis in a Chinese population

“…In the present study, the gene polymorphism of MCP‐1 was analyzed. The genotype and allele frequencies were similar to those of previously reported studies in healthy Chinese subjects (16,17), and those in Japanese (18) and Korean (19) subjects. The genotype and allele frequencies in Chinese subjects were obviously different from those of African–American, Caucasian and Hispanic subjects (11,12,20); the G‐allele frequency was increased in Chinese subjects.…”

Association of Monocyte Chemoattractant Protein-1 −2518 Polymorphism With Metabolic Syndrome in a South Indian Cohort

“…A multivariate linear regression analysis was performed to (27%) 10 ; however, it is quite different to that reported in the Korean population (60%) 22 and Japanese population (71%). 23 In our study population, the MCP-1 -2518G allele was observed to be associated with higher MCP-1 levels as previously observed. 10,23, 24 We did not, however, observe any correlation between MCP-1 levels and metabolic syndrome or its individual components.…”

Association of FCGR2A p.R131H and CCL2 c.-2518 A>G gene variants with thrombocytopenia in patients with dengue virus infection

“…DNA was isolated from the whole blood and genotyping of FCGR2A p.R131H (rs1801274) and CCL2 c.-2518 A > G (rs1024611) polymorphisms were investigated using a PCR-restriction fragment length based polymorphism method as described earlier [11,12].…”