mcr-1 encoding colistin resistance in CTX-M-1/CTX-M-15- producing Escherichia coli isolates of bovine and caprine origins in Tunisia. First report of CTX-M-15-ST394/D E. coli from goats

Hassen, Bilel; Benlabidi, Saloua; Abbassi, Mohamed Salah; Ruiz-Ripa, Laura; Mama, Olouwafemi Mistourath; Hassen, Abdennaceur; Hammami, Salah; Torres, Carmén

doi:10.1016/j.cimid.2019.101366

Cited by 36 publications

(35 citation statements)

References 44 publications

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“…Moreover, mcr genes are being detected in enterobacterial isolates with other resistance genes. In Tunisia, E. coli isolates with both CTX-M-15 and mcr-1 were recovered from bovine fecal samples and raw goat milk [ 179 ]. In Brazil, the emergence of a K. pneumoniae isolate with both bla KPC and mcr-1 was reported [ 180 ]; likewise, the same gene combination was found in Enterobacteriaceae growing in streams and wastewater treatment plants in Italy [ 181 ].…”

Section: Special Features and Spread Of Polymyxin Resistance Amongmentioning

confidence: 99%

Polymyxins and Bacterial Membranes: A Review of Antibacterial Activity and Mechanisms of Resistance

2020

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Following their initial discovery in the 1940s, polymyxin antibiotics fell into disfavor due to their potential clinical toxicity, especially nephrotoxicity. However, the dry antibiotic development pipeline, together with the rising global prevalence of infections caused by multidrug-resistant (MDR) Gram-negative bacteria have both rejuvenated clinical interest in these polypeptide antibiotics. Parallel to the revival of their use, investigations into the mechanisms of action and resistance to polymyxins have intensified. With an initial known effect on biological membranes, research has uncovered the detailed molecular and chemical interactions that polymyxins have with Gram-negative outer membranes and lipopolysaccharide structure. In addition, genetic and epidemiological studies have revealed the basis of resistance to these agents. Nowadays, resistance to polymyxins in MDR Gram-negative pathogens is well elucidated, with chromosomal as well as plasmid-encoded, transferrable pathways. The aims of the current review are to highlight the important chemical, microbiological, and pharmacological properties of polymyxins, to discuss their mechanistic effects on bacterial membranes, and to revise the current knowledge about Gram-negative acquired resistance to these agents. Finally, recent research, directed towards new perspectives for improving these old agents utilized in the 21st century, to combat drug-resistant pathogens, is summarized.

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Section: Special Features and Spread Of Polymyxin Resistance Amongmentioning

confidence: 99%

Polymyxins and Bacterial Membranes: A Review of Antibacterial Activity and Mechanisms of Resistance

2020

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“…The presence of MGCB in the beef/dairy cattle sector in Africa has been reported [ 21 , 25 , 35 , 50 ]. mcr -1 gene was detected in eight COL-resistant E .…”

Section: Plasmid-mediated Colistin Resistance Among Isolates From mentioning

confidence: 99%

“…In Tunisia, 14 faecal strains carrying mcr -1 on IncP, IncHI, and IncFIB plasmids and class 1 integrons were detected among 25 ESBL-producing E . coli (56%) isolated from cattle [ 35 , 50 ], suggesting that there is a high prevalence of mcr -1-habouring E . coli in the Tunisian bovine sector, and that diverse MGEs evolved mcr -1 and ESBL genes in commensal and virulent E .…”

Section: Plasmid-mediated Colistin Resistance Among Isolates From mentioning

confidence: 99%

“…coli clones in North Africa. Four other resistance genes (including ESBL gene) in three antimicrobial families were also present in the mcr -1-positive strains [ 35 , 50 ] ( Table 1 ), suggesting that virulent multiresistant E . coli strains are colonizing food-producing animals in the Mediterranean region.…”

Section: Plasmid-mediated Colistin Resistance Among Isolates From mentioning

confidence: 99%

“…Inadequate husbandry and slaughterhouse facilities and unhygienic slaughtering techniques facilitate the contamination of animal-related products in Africa [ 53 ]. Six publications investigated the mcr gene in 211 isolates from foods of animal origin in Africa [ 36 , 50 , 54 – 57 ]. Twenty-nine strains were reported to harbour mcr -1 among the isolates tested.…”

Section: Plasmid-mediated Colistin Resistance Among Isolates From mentioning

confidence: 99%

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Prevalence and Traits of Mobile Colistin Resistance Gene Harbouring Isolates from Different Ecosystems in Africa

Anyanwu

Okpala

Chah

et al. 2021

BioMed Research International

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The mobile colistin resistance (mcr) gene threatens the efficacy of colistin (COL), a last-line antibiotic used in treating deadly infections. For more than six decades, COL is used in livestock around the globe, including Africa. The use of critically important antimicrobial agents, like COL, is largely unregulated in Africa, and many other factors militate against effective antimicrobial stewardship in the continent. Currently, ten mcr genes (mcr-1 to mcr-10) have been described. In Africa, mcr-1, mcr-2, mcr-3, mcr-5, mcr-8, and mcr-9 have been detected in isolates from humans, animals, foods of animal origin, and the environment. These genes are harboured by Escherichia coli, Klebsiella, Salmonella, Citrobacter, Enterobacter, Pseudomonas, Aeromonas, Alcaligenes, and Acinetobacter baumannii isolates. Different conjugative and nonconjugative plasmids form the backbone for mcr in these isolates; however, mcr-1 and mcr-3 have also been integrated into the chromosome of some African strains. Insertion sequences (ISs) (especially ISApl1), either located upstream or downstream of mcr, class 1 integrons, and transposons, are drivers of mcr in Africa. Genes coding multi/extensive drug resistance and virulence are colocated with mcr on plasmids in African strains. Transmission of mcr to/among African strains is nonclonal. Contact with mcr-habouring reservoirs, the consumption of contaminated foods of animal/plant origin or fluid, animal-/plant-based food trade and travel serve as exportation, importation, and transmission routes of mcr gene-containing bacteria in Africa. Herein, the current status of plasmid-mediated COL resistance in humans, food-producing animals, foods of animal origin, and environment in Africa is discussed.

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Genetic characterization of ESBL-producing Escherichia coli and Klebsiella pneumoniae isolated from wastewater and river water in Tunisia: predominance of CTX-M-15 and high genetic diversity

Hassen

Abbassi

Benlabidi

et al. 2020

Environ Sci Pollut Res

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mcr-1 encoding colistin resistance in CTX-M-1/CTX-M-15- producing Escherichia coli isolates of bovine and caprine origins in Tunisia. First report of CTX-M-15-ST394/D E. coli from goats

Cited by 36 publications

References 44 publications

Polymyxins and Bacterial Membranes: A Review of Antibacterial Activity and Mechanisms of Resistance

Polymyxins and Bacterial Membranes: A Review of Antibacterial Activity and Mechanisms of Resistance

Prevalence and Traits of Mobile Colistin Resistance Gene Harbouring Isolates from Different Ecosystems in Africa

Genetic characterization of ESBL-producing Escherichia coli and Klebsiella pneumoniae isolated from wastewater and river water in Tunisia: predominance of CTX-M-15 and high genetic diversity

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