MdbHLH3 modulates apple soluble sugar content by activating phosphofructokinase gene expression

Yu, Jian‐Qiang; Gu, Ke; Zhang, Lili; Sun, Chuanqing; Zhang, Quan‐Yan; Wang, Jiahui; Wang, Chukun; Wang, Wenyan; Du, Meng‐Chi; D, Hu

doi:10.1111/jipb.13236

Cited by 39 publications

(11 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The IAA–LEUCINE RESISTANT 3 ( Rh ILR3; Rh bHLH105)–LESION SIMULATING DISEASE ONE LIKE 1 ( Rh LOL1) module regulates petal abscission by directly binding to the RhIAA4-1 promoter in roses [ 53 ]. Additionally, MdbHLH3 regulates sugar metabolism in apples by binding directly to the promoter of PYROPHOSPHATE-DEPENDENT PHOSPHOFRUCTOKINASE ( MdPFPβ ) to activate its expression, thereby increasing fructose-6-phosphate and sucrose concentrations [ 54 ]. In rice, OsbHLH067, OsbHLH068, and OsbHLH069 redundantly regulate inflorescence AM formation [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Auxin regulates bulbil initiation by mediating sucrose metabolism in Lilium lancifolium

Xin,

Chen,

Liang

et al. 2024

Horticulture Research

View full text Add to dashboard Cite

Lily bulbils, which serve as advantageous axillary organs for vegetative propagation, have not been extensively studied in terms of the mechanism of bulbil initiation. The function of auxin and sucrose metabolism have been implicated in axillary organ development, but their relationship in regulating bulbil initiation remains unclear. In this study, exogenous indole-3-acetic acid (IAA) treatment increased the endogenous auxin levels at leaf axils and significantly decreased bulbil number, whereas treatment with the auxin polar transport inhibitor N-1-naphthylphthalamic acid (NPA), which resulted in a low auxin concentration at leaf axils, stimulated bulbil initiation and increased bulbil number. A low level of auxin caused by NPA spraying or silencing of auxin biosynthesis genes YUCCA FLAVIN MONOOXYGENASE-LIKE6 (LlYUC6) and TRYPTOPHAN AMINO-TRANSFERASE1 (LlTAR1) facilitated sucrose metabolism by activating the expression of LlSusy1 and LlCWIN2, resulting in enhanced bulbil initiation. Silencing SUCROSE SYNTHASES1 (LlSusy1) or CELL WALL INVERTASE2 (LlCWIN2) hindered bulbil initiation. Moreover, the transcription factor BASIC-HELIX-LOOP-HELIX35 (LlbHLH35) directly bound the promoter of LlSusy1, but not the promotor of LlCWIN2, and activated its transcription in response to the auxin content, bridging the gap between auxin and sucrose metabolism. In conclusion, our results reveal a LlbHLH35-LlSusy1 module mediates auxin-regulated sucrose metabolism during bulbil initiation.

show abstract

Section: Discussionmentioning

confidence: 99%

Auxin regulates bulbil initiation by mediating sucrose metabolism in Lilium lancifolium

Xin,

Chen,

Liang

et al. 2024

Horticulture Research

View full text Add to dashboard Cite

show abstract

“…Considering that PpS6PDH is a reversible enzyme, its role in fruit ripening requires further investigation. In apples, activation of a pyrophosphate-dependent phosphofructokinase gene can promote soluble sugar accumulation ( Yu et al., 2022 ). In sweet pear cultivars evaluated in the present study, the gene encoding 6-phosphofructokinase 7 was upregulated.…”

Section: Discussionmentioning

confidence: 99%

QTL mapping and transcriptome analysis of sugar content during fruit ripening of Pyrus pyrifolia

Jiang

Luo

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

Sugar content is an important trait of fruits. The genetic background of fruits can affect their sugar content in different cultivars. The quantitative trait loci and genes related to sugar content during fruit ripening remain unclear. In this study, we performed quantitative trait locus (QTL) mapping of sugar content. Two QTLs (qSugar-LG6-Chr7 and qSugar-LG12-Chr3) were identified based on their total sugar contents. A total of 577 and 519 genes were annotated around these two QTL loci. The contents of fructose, sorbitol, glucose, and sucrose were measured at six time points in four cultivars before fruit maturation, including two sweet cultivars (“Zaoshengxinshui” and “ZQ65”) and two general cultivars (“Qiushui” and “ZQ82”). In sweet cultivars, sucrose and fructose accumulate substantially, and sorbitol content decreases significantly during fruit ripening. A transcriptome analysis identified 125 upregulated and 222 downregulated differentially expressed genes (DEGs) in sweet cultivars. Two sucrose transport genes (PpSUT, LOC103964096, and LOC103940043) were negatively correlated with sugar content. A weighted gene co-expression network analysis showed that two genes, sorbitol dehydrogenase (PpSDH, LOC103960512 and LOC103960513), around the locus of qSugar-LG6-Chr7 were negatively co-expressed with the total sugar content, which was downregulated in the sweet cultivars. PpSDH and PpSUT may play important roles in regulating sugar content during pear ripening. Transcriptome analysis also revealed that some DEGs were related to sugars (PpS6PDH and ATP-PpPFK), hormones (PpARG7), and transcription factors (PpEMB1444, PpCYP734A1, and PpWRKY50). In conclusion, this study provides new insights into the molecular mechanisms associated with sugar content in the fruit ripening of Pyrus pyrifolia.

show abstract

“…A phosphofructokinase: pyrophosphate-fructose 6-phosphate 1-phosphotransferase (ClPFP1, Cla97C11G225220) was found. Previous study reported that PFP activity showed a positive correlation with sucrose content in apple fruit (Yu et al, 2022). We therefore hypothesized that ClSnRK2.3 may directly phosphorylate ClPFP1 to regulate watermelon fruit sugar accumulation.…”

Section: Clsnrk23 Phosphorylated Phosphofructokinase Clpfp1 the Ga Bi...mentioning

confidence: 91%

ClSnRK2.3 negatively regulates watermelon fruit ripening and sugar accumulation

Wang

et al. 2023

JIPB

View full text Add to dashboard Cite

Watermelon (Citrullus lanatus) as non‐climacteric fruit is domesticated from the ancestors with inedible fruits. We previously revealed that the abscisic acid (ABA) signaling pathway gene ClSnRK2.3 might influence watermelon fruit ripening. However, the molecular mechanisms are unclear. Here, we found that the selective variation of ClSnRK2.3 resulted in lower promoter activity and gene expression level in cultivated watermelons than ancestors, which indicated ClSnRK2.3 might be a negative regulator in fruit ripening. Overexpression (OE) of ClSnRK2.3 significantly delayed watermelon fruit ripening and suppressed the accumulation of sucrose, ABA and gibberellin GA4. Furthermore, we determined that the pyrophosphate‐dependent phosphofructokinase (ClPFP1) in sugar metabolism pathway and GA biosynthesis enzyme GA20 oxidase (ClGA20ox) could be phosphorylated by ClSnRK2.3 and thereby resulting in accelerated protein degradation in OE lines and finally led to low levels of sucrose and GA4. Besides that, ClSnRK2.3 phosphorylated homeodomain‐leucine zipper protein (ClHAT1) and protected it from degradation to suppress the expression of the ABA biosynthesis gene 9’‐cis‐epoxycarotenoid dioxygenase 3 (ClNCED3). These results indicated that ClSnRK2.3 negatively regulated watermelon fruit ripening by manipulating the biosynthesis of sucrose, ABA and GA4. Altogether, these findings revealed a novel regulatory mechanism in non‐climacteric fruit development and ripening.

show abstract

MdbHLH3 modulates apple soluble sugar content by activating phosphofructokinase gene expression

Cited by 39 publications

References 51 publications

Auxin regulates bulbil initiation by mediating sucrose metabolism in Lilium lancifolium

Auxin regulates bulbil initiation by mediating sucrose metabolism in Lilium lancifolium

QTL mapping and transcriptome analysis of sugar content during fruit ripening of Pyrus pyrifolia

ClSnRK2.3 negatively regulates watermelon fruit ripening and sugar accumulation

Contact Info

Product

Resources

About