MDC1 depletion promotes cisplatin induced cell death in cervical cancer cells

Singh, Neeru; Bhakuni, Rashmi; Chhabria, Dimple; Kirubakaran, Sivapriya

doi:10.1186/s13104-020-04996-5

Cited by 12 publications

(5 citation statements)

References 28 publications

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“…The sophoridine is a plant derived-natural compound that enhances sensitivity of lung cancer cells into cisplatin via induction of p53 and Hippo signaling pathways(Zhu et al, 2020). The mediator of DNA damage checkpoint 1 (MDC1) is a genetic factor that its down-regulation induces apoptosis in cervical cancer cells(Singh, Bhakuni, Chhabria, & Kirubakaran, 2020). It is worth mentioning that CT can target molecular pathways to increase cisplatin sensitivity.…”

mentioning

confidence: 99%

Recent advances and future directions in anti‐tumor activity of cryptotanshinone: A mechanistic review

Ashrafizadeh

Zarrabi

Orouei

et al. 2020

Phytotherapy Research

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In respect to the enhanced incidence rate of cancer worldwide, studies have focused on cancer therapy using novel strategies. Chemotherapy is a common strategy in cancer therapy, but its adverse effects and chemoresistance have limited its efficacy. So, attempts have been directed towards minimally invasive cancer therapy using plant derived‐natural compounds. Cryptotanshinone (CT) is a component of salvia miltiorrihiza Bunge, well‐known as Danshen and has a variety of therapeutic and biological activities such as antioxidant, anti‐inflammatory, anti‐diabetic and neuroprotective. Recently, studies have focused on anti‐tumor activity of CT against different cancers. Notably, this herbal compound is efficient in cancer therapy by targeting various molecular signaling pathways. In the present review, we mechanistically describe the anti‐tumor activity of CT with an emphasis on molecular signaling pathways. Then, we evaluate the potential of CT in cancer immunotherapy and enhancing the efficacy of chemotherapy by sensitizing cancer cells into anti‐tumor activity of chemotherapeutic agents, and elevating accumulation of anti‐tumor drugs in cancer cells. Finally, we mention strategies to enhance the anti‐tumor activity of CT, for instance, using nanoparticles to provide targeted drug delivery.

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mentioning

confidence: 99%

Recent advances and future directions in anti‐tumor activity of cryptotanshinone: A mechanistic review

Ashrafizadeh

Zarrabi

Orouei

et al. 2020

Phytotherapy Research

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“…Next, we turned our attention to explore the activity of splice modulation on cell lines displaying sensitivity or resistance to cisplatin, as the latter is an issue in the treatment of solid tumors including ovarian [ 38 ], cervical cancer [ 39 ], gastric adenocarcinoma [ 40 ], prostate cancer [ 41 ], colorectal [ 42 ], and head and neck squamous cell carcinoma [ 43 ]. Here, we used two human ovarian cancer cell lines, one consisting of a cisplatin-sensitive parental line, OV2008, and the other stably cisplatin-resistant subline, OV2008/C13 derived by in vitro selection with cisplatin.…”

Section: Resultsmentioning

confidence: 99%

Modulation of RNA splicing associated with Wnt signaling pathway using FD-895 and pladienolide B

Kumar

Kashyap

et al. 2022

Aging

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Alterations in RNA splicing are associated with different malignancies, including leukemia, lymphoma, and solid tumors. The RNA splicing modulators such as FD-895 and pladienolide B have been investigated in different malignancies to target/modulate spliceosome for therapeutic purpose. Different cell lines were screened using an RNA splicing modulator to test in vitro cytotoxicity and the ability to modulate RNA splicing capability via induction of intron retention (using RT-PCR and qPCR). The Cignal Finder Reporter Array evaluated [pathways affected by the splice modulators in HeLa cells. Further, the candidates associated with the pathways were validated at protein level using western blot assay, and gene-gene interaction studies were carried out using GeneMANIA. We show that FD-895 and pladienolide B induces higher apoptosis levels than conventional chemotherapy in different solid tumors. In addition, both agents modulate Wnt signaling pathways and mRNA splicing. Specifically, FD-895 and pladienolide B significantly downregulates Wnt signaling pathway-associated transcripts (GSK3β and LRP5) and both transcript and proteins including LEF1, CCND1, LRP6, and pLRP6 at the transcript, total protein, and protein phosphorylation's levels. These results indicate FD-895 and pladienolide B inhibit Wnt signaling by decreasing LRP6 phosphorylation and modulating mRNA splicing through induction of intron retention in solid tumors.

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“…Many of the 33 genes in the GSE142699 dataset identified as targets of miR-199a-5p have been reported to be associated with cancer and AML. For example, the MDC1 gene, which was determined as the hub gene among these genes, was found to be closely related to many cancers (27,28). In the study by Ruff et al, it was suggested that MDC1 may be an important biomarker in carcinogenesis (29).…”

Section: Discussionmentioning

confidence: 99%

A Bioinformatics Analysis of circRNA/miRNA/mRNA Interactions in Acute Myeloid Leukemia

Erdoğan¹,

Kaya²,

Suer³

2023

experimed

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Objective: Acute myeloid leukemia (AML) is a lethal type of cancer associated with dysregulation of progenitor hematopoietic stem cell behavior and its incidence is, unfortunately, increasing. Although there are various applications in treatment, since most of them are insufficient in early diagnosis, treatment and new prognostic biomarkers should be investigated. Materials and Methods:In this study, three Gene Expression Omnibus (GEO) datasets Genomic Spatial Event (GSE); GSE94591, GSE116617, and GSE163386) were used to investigate dysregulated expressions of circular RNAs (circRNAs), and the GSE142699 and GSE142698 datasets were analyzed to detect dysregulated expressions of microRNAs (miRNAs) and mRNAs, respectively. Filtering was applied with p value <0.05, log2FC≥0.5 (circRNA), and log2FC≥1 (miRNA and mRNA) from the raw data analyzed using the limma R package (v.3.46.0). We investigated circRNA-miRNA-mRNA interactions using special tools including CSCDv2.0, circBank, miRTarBase, miRDB, multiMiR, miRWalk, DIANA-microT, TarBase, miRanda, and TargetScan. The pathway analysis was performed using KEGG and GO programs. The STRING database and Cytoscape tool were used to construct and view protein interaction. Hub gene analysis was constructed using the MCODE tool. We have utilized the GEPIA tool to determine the Overall Survival of the hub genes.Results: In our study, 4 circRNAs, 3 miRNAs, and 6 genes that may be closely related to AML were detected. Conclusion:According to our bioinformatics analysis results, hsa_circ_0012152/miR-199a-5p/HOXA9 axis could be more important in AML. Therefore, in vitro and in vivo investigations are recommended.

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MDC1 depletion promotes cisplatin induced cell death in cervical cancer cells

Cited by 12 publications

References 28 publications

Recent advances and future directions in anti‐tumor activity of cryptotanshinone: A mechanistic review

Recent advances and future directions in anti‐tumor activity of cryptotanshinone: A mechanistic review

Modulation of RNA splicing associated with Wnt signaling pathway using FD-895 and pladienolide B

A Bioinformatics Analysis of circRNA/miRNA/mRNA Interactions in Acute Myeloid Leukemia

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