MDM2 polymorphism associated with the development of cervical lesions in women infected with Human papillomavirus and using of oral contraceptives

Abstract

BackgroundThe MDM2 gene is the major negative regulator of p53, a tumor suppressor protein. Single nucleotide polymorphism in promoter region of MDM2 gene leads to increased expression resulting in higher levels of MDM2 protein. This event increases the attenuation of the p53 pathway. Polymorphisms in this gene can interfere in the regulation of cellular proliferation. We evaluated whether MDM2 SNP309 (rs2278744) associated or not with the use of oral contraceptive can heighten susceptibility to development of cervical lesions in women HPV infected.MethodsMDM2 SNP309 (rs2278744) was genotyped in a total of 287 patients using the PCR-RFLP technique. The results were analyzed by UNPHASED v.3.121 and SNPStats programs.ResultsThe three groups (SIL, LSIL and HSIL) showed no significant differences in either genotype or allelic frequencies for MDM2 polymorphisms, except when HSIL was compared with LSIL (p = 0.037; OR = 1.81). Furthermore, in the analysis of contraceptives, a significant association was found between the use of contraceptives and the MDM2 variant in the development of high-grade cervical lesions for the TG genotype (p = 0.019; OR = 2.21) when HSIL was compared with control. When HSIL was compared with LSIL (p = 0.006; OR = 2.27).ConclusionThe results of this study suggest that MDM2 SNP309 might be a good marker for assessing the progression of LSIL to HSIL. In addition, they also show that oral contraceptives alone, did not have any effect on the progression or development of cervical lesions. However, they may act synergistically with MDM2 SNP309 (rs2278744) and HPV infection in the development of cervical lesions.

Prevalence of human papillomavirus (HPV), distribution of HPV types, and risk factors for infection in HPV-positive women

“…When patients were stratified by HPV samples, we observed an association between HPV positivity, the use of contraceptives, and the number of sexual partners. The results of this study corroborate previous findings from other studies that have shown a link between the use of contraceptives and HR HPV infection (Finan et al, 2002;Au, 2004;Chagas et al, 2013;Amaral et al, 2014). Therefore, our results suggest that behavioral factors might have an impact on the status of cervical cytology in our study population.…”

Labeling of HeLa cells using ZrO 2 : Yb 3 + - Er 3 + nanoparticles with upconversion emission

“…In 2012, according to World Health Organization, cervical cancer was one of the most prevalent cancer types in the world. To detect and diagnose cancer, there are several biomarkers; [20][21][22][23] for example, the Ki-67 protein is expressed in all phases of the cell division cycle, but its expression level is strongly downregulated in the resting G0 phase. This characteristic makes the Ki-67 protein an excellent biomarker for cell proliferation.…”

Fatores associados às alterações cérvico-uterinas de mulheres atendidas em um município polo do oeste catarinense Factors associated to uterine-cervix changes in women assisted in a pole town in western Santa Catarina

“…34 The oral contraceptives alone have no effect on the progression or development of cervical lesions, but they can act in synergy, increasing the susceptibility to develop highdegree lesions in women infected with HPV. 35 Also, they can result in higher frequency of unprotected sexual intercourse, putting the women at risk of acquiring other STDs. 36 As regards the epithelium represented in the sample, it was possible to observe that women that had metaplastic epithelium had 1.3 times more chances to develop cellular changes in the cervix.…”