Measurement of Complement Proteins C2 and B in Systemic Lupus erythematosus and Septic Shock

Oglesby, Teresa J.; Schultz, Duane R.; Schein, Roland M. H.; Sprung, Charles L.; Volanakis, John E.

doi:10.1159/000463069

Cited by 6 publications

(6 citation statements)

References 19 publications

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“…In these patients, the alternative pathway appears to be activated early in septic shock, whereas the classical pathway is activated later. Oglesby et al also reported that among septic shock patients (both Gram-negative and Gram-positive) with preexisting cirrhosis, factor B levels were decreased [34]. Lin et al reported that the active fragment of factor B, Bb as well as the Bb to factor B ratio, was significantly increased in septic shock patients [29].…”

Section: Pathophysiology Of Complement Involvement In Septic Shockmentioning

confidence: 99%

The Role of Complement System in Septic Shock

Charchaflieh

Wei

Labaze

et al. 2012

Clinical and Developmental Immunology

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Septic shock is a critical clinical condition with a high mortality rate. A better understanding of the underlying mechanisms is important to develop effective therapies. Basic and clinical studies suggest that activation of complements in the common cascade, for example, complement component 3 (C3) and C5, is involved in the development of septic shock. The involvement of three upstream complement pathways in septic shock is more complicated. Both the classical and alternative pathways appear to be activated in septic shock, but the alternative pathway may be activated earlier than the classical pathway. Activation of these two pathways is essential to clear endotoxin. Recent investigations have shed light on the role of lectin complement pathway in septic shock. Published reports suggest a protective role of mannose-binding lectin (MBL) against sepsis. Our preliminary study of MBL-associated serine protease-2 (MASP-2) in septic shock patients indicated that acute decrease of MASP-2 in the early phase of septic shock might correlate with in-hospital mortality. It is unknown whether excessive activation of these three upstream complement pathways may contribute to the detrimental effects in septic shock. This paper also discusses additional complement-related pathogenic mechanisms and intervention strategies for septic shock.

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Section: Pathophysiology Of Complement Involvement In Septic Shockmentioning

confidence: 99%

The Role of Complement System in Septic Shock

Charchaflieh

Wei

Labaze

et al. 2012

Clinical and Developmental Immunology

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“…To quantify the C2, factor B and factor D secreted by U-105-MG cells, solid-phase RIMAs were performed as previously described [21,22]. All three RIMAs utilize rabbit polyclonal antibodies in the solid phase and 125I-labelled mouse mAb as reporters.…”

Section: Radioimmunometric Assays (Rimas)mentioning

confidence: 99%

Production and interferon-γ-mediated regulation of complement component C2 and factors B and D by the astroglioma cell line U105-MG

Barnum

Ishii

Agrawal

et al. 1992

Biochemical Journal

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In this paper, we demonstrate the synthesis of the complement component C2 and factors B and D by the human astroglioma cell line U105-MG. All three components were structurally and antigenically similar to their serum counterparts, as determined by biosynthetic labelling studies or Western blot analysis. Northern blot analysis demonstrated that the mRNAs of all three components had the same apparent sizes as the equivalent mRNAs from hepatocyte and monocyte cell lines. Interestingly, U105-MG cells produce two C2 transcripts with sizes of approximately 2.8 and 2.3 kb. Interferon-gamma (IFN-gamma) enhanced the expression of C2 and factor B mRNA and protein in a dose- and time-dependent fashion, while factor D expression was refractory to IFN-gamma. IFN-gamma appeared to predominantly enhance the expression of the large (2.8 kb) C2 transcript. Kinetic studies demonstrated peak C2 and factor B expression in 48 h in response to IFN-gamma, similar to the acute-phase response of factor B in serum. These data are the first to demonstrate the synthesis of C2 and factor D by astroglioma cells. Combined with previous reports documenting the synthesis of C3 by astrocytes, our data suggest that endogenous synthesis of complement proteins, and particularly of alternative pathway activation components (C3, factors B and D), may play an important role in host defence in the central nervous system.

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“…Serum from ambulatory SLE patients was collected on rou tinely scheduled visits to the University of Alabama Clinics. The clinical parameters of these individuals have been previously described [12], All the SLE patients were female, and none had severely active disease based on the scale of Valentijn et al [32] which includes physical examination and laboratory studies (complete blood count, platelet count, blood urea nitrogen, creatinine, and urinanalysis). No pa tient had more than three organ systems involved.…”

Section: Serum Samplesmentioning

confidence: 99%

“…The wide range of C4BP serum concentrations in nor mal individuals is not unusual when com pared to other complement proteins. For ex ample, complement protein D [38], Clq, Cls, C3, C4, C5, C9 [39], C2, and B [12], all have wide ranges in normal serum.…”

Section: Serum Samplesmentioning

confidence: 99%

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C4b-Binding Protein, a Regulatory Component of the Classical Pathway of Complement, Is an Acute-Phase Protein and Is Elevated in Systemic Lupus Erythematosus

Barnum¹,

Dahlbäck²

1990

Complement Inflamm

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A radioimmunoassay using monoclonal and polyclonal antihuman C4b-binding protein (C4BP) antibody was developed to quantitate C4BP in serum. Using the assay, the levels of C4BP in healthy individuals, in patients with systemic lupus erythematosus (SLE), and in acute-phase individuals were determined. The levels of C4BP are significantly elevated in individuals with SLE (186%; p = 0.0001) and are even higher in individuals during the acute phase (286%; p = 0.0001). To confirm whether or not individuals were in the acute-phase response, serum C-reactive protein (CRP) levels were assessed. In the acute- phase response, CRP levels were 100-fold elevated over normals, but did not correlate with increases in C4BP (r = -0.031; p = 0.899). In SLE patients, the CRP levels were significantly, but moderately, elevated (5-fold; p = 0.028). The data indicate that C4BP is an acute-phase reactant and is differentially regulated from CRP during the acute-phase response.

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Measurement of Complement Proteins C2 and B in Systemic Lupus erythematosus and Septic Shock

Cited by 6 publications

References 19 publications

The Role of Complement System in Septic Shock

The Role of Complement System in Septic Shock

Production and interferon-γ-mediated regulation of complement component C2 and factors B and D by the astroglioma cell line U105-MG

C4b-Binding Protein, a Regulatory Component of the Classical Pathway of Complement, Is an Acute-Phase Protein and Is Elevated in Systemic Lupus Erythematosus

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