Measurement of Metabolites from Migrating Cells

Williams, Demond; Fingleton, Barbara

doi:10.1007/978-1-0716-1350-4_10

Methods in Molecular Biology

2021

DOI: 10.1007/978-1-0716-1350-4_10

|View full text |Cite

Measurement of Metabolites from Migrating Cells

Demond Williams

Barbara Fingleton

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Deep and dynamic metabolic and structural imaging in living tissues

Liu,

Cao,

Shashaty

et al. 2024

Sci. Adv.

View full text Add to dashboard Cite

Label-free imaging through two-photon autofluorescence of NAD(P)H allows for nondestructive, high-resolution visualization of cellular activities in living systems. However, its application to thick tissues has been restricted by its limited penetration depth within 300 μm, largely due to light scattering. Here, we demonstrate that the imaging depth for NAD(P)H can be extended to more than 700 μm in living engineered human multicellular microtissues by adopting multimode fiber-based, low repetition rate, high peak power, three-photon excitation of NAD(P)H at 1100 nm. This is achieved by having more than 0.5 megawatts peak power at the band of 1100 ± 25 nm through adaptively modulating multimodal nonlinear pulse propagation with a compact fiber shaper. Moreover, the eightfold increase in pulse energy enables faster imaging of monocyte behaviors in the living multicellular models. These results represent a substantial advance for deep and dynamic imaging of intact living biosystems. The modular design is anticipated to allow wide adoption for demanding imaging applications, including cancer research, immune responses, and tissue engineering.

show abstract

Deep and dynamic metabolic and structural imaging in living tissues

Liu,

Cao,

Shashaty

et al. 2024

Sci. Adv.

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Measurement of Metabolites from Migrating Cells

Cited by 1 publication

References 4 publications

Deep and dynamic metabolic and structural imaging in living tissues

Deep and dynamic metabolic and structural imaging in living tissues

Contact Info

Product

Resources

About