Measurement of Neutrophil Pulmonary Transit Time in Humans.

Summers, Charlotte; White, J R; Mackenzie, I.A.R.; Solanki, Chandra K.; Am, Peters; Chilvers, Edwin R.

doi:10.1164/ajrccm-conference.2009.179.1_meetingabstracts.a1333

Cited by 4 publications

(6 citation statements)

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“…Where 51 Cr-labeled cells revealed a neutrophil half-life of 17.5 h, the ex vivo DFP and 3 HTdr labeling studies all came to similar half-lives of 5-9 h in peripheral blood (Table 1) [8,16,17,32,33]. Despite the relatively consistent picture that emerges from these studies, ex vivo labeling has a major limitation, as it causes neutrophil activation, which has been shown to alter neutrophil homing characteristics [19,22,29,38] and thereby affects the neutrophil circulatory half-life. Indeed, ex vivo manipulation of neutrophils has been shown to have dramatic effects on their circulatory half-life in mice; in vivo-labeled neutrophils had a half-life of 8 -10 h, whereas ex vivo-labeled neutrophils disappeared from the blood with a half-life of only 1.5 h [24,39].…”

Section: Ex Vivo Labelingmentioning

confidence: 90%

“…The lungs have also been implicated as a site for neutrophil margination [20], but recent studies have questioned this notion by showing that the transit time through the lungs was dependent on the method of isolation of the neutrophils and their activation state [19,22]. Neutrophils isolated by the method that induced the least activation showed a mere 3.5-s longer pulmonary transit time than red blood cells [19,22]. Besides influencing the site of margination, ex vivo manipulation of neutrophils was shown to influence the size of the marginated pool, with an increase of margination proportional to the duration of the labeling procedure [16].…”

Section: The Marginated Poolmentioning

confidence: 99%

“…In addition, neutrophils were shown to take 2-10 min to transit through these organs-much longer than expected if they were to flow freely through the blood vessels [20,21]. The lungs have also been implicated as a site for neutrophil margination [20], but recent studies have questioned this notion by showing that the transit time through the lungs was dependent on the method of isolation of the neutrophils and their activation state [19,22]. Neutrophils isolated by the method that induced the least activation showed a mere 3.5-s longer pulmonary transit time than red blood cells [19,22].…”

Section: The Marginated Poolmentioning

confidence: 99%

See 2 more Smart Citations

What's your age again? Determination of human neutrophil half-lives revisited

Tak

Tesselaar

Pillay

et al. 2013

Journal of Leukocyte Biology

242

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Neutrophils are the most abundant white blood cells and are indispensable for host defense. Recently, they have also been implicated in immune regulation and suppression. The latter functions seem hard to reconcile with the widely held view that neutrophils are very short-lived, with a circulatory half-life of <7 h. To reopen the discussion on the average neutrophil half-life, we review and discuss experiments performed in the 1950s, 1960s, and 1970s, as well as recent in vivo labeling experiments. We reappraise the current knowledge on neutrophil half-lives, including their production in the bone marrow, their residency in the circulation and marginated pool, and their exit from the circulation.

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Section: Ex Vivo Labelingmentioning

confidence: 90%

Section: The Marginated Poolmentioning

confidence: 99%

Section: The Marginated Poolmentioning

confidence: 99%

See 1 more Smart Citation

What's your age again? Determination of human neutrophil half-lives revisited

Tak

Tesselaar

Pillay

et al. 2013

Journal of Leukocyte Biology

242

185

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“…In addition, in mice, about 10 % of reinfused radiolabeled neutrophils migrated towards the spleen, which was not influenced by the maturation status of neutrophils or inflammation [101]. It is important to emphasize that ex vivo manipulation of the cells could have induced subtle changes affecting their homing behavior in vivo [102]. …”

Section: Distribution Of Neutrophils and G-mdscs In Lymphoid Organsmentioning

confidence: 99%

Immune suppression by neutrophils and granulocytic myeloid-derived suppressor cells: similarities and differences

Pillay

Tak

Kamp

et al. 2013

Cell. Mol. Life Sci.

333

339

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Neutrophils are essential effector cells in the host defense against invading pathogens. Recently, novel neutrophil functions have emerged in addition to their classical anti-microbial role. One of these functions is the suppression of T cell responses. In this respect, neutrophils share similarities with granulocytic myeloid-derived suppressor cells (G-MDSCs). In this review, we will discuss the similarities and differences between neutrophils and G-MDSCs. Various types of G-MDSCs have been described, ranging from immature to mature cells shaping the immune response by different immune suppressive mechanisms. However, all types of G-MDSCs share distinct features of neutrophils, such as surface markers and morphology. We propose that G-MDSCs are heterogeneous and represent novel phenotypes of neutrophils, capable of suppressing the immune response. In this review, we will attempt to clarify the differences and similarities between neutrophils and G-MDSCs and attempt to facilitate further research.

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“…12 Ex vivo manipulation has been shown to have dramatic effects on neutrophil redistribution in vivo. 13 In mice, half-lives of 8 to 10 hours were reported when neutrophils were labeled in vivo. 14 In contrast, ex vivo labeling in mice showed that after transfer 90% of labeled neutrophils were cleared from the circulation within 4 hours, resulting in a half-life of less than 1.5 hours.…”

Section: Introductionmentioning

confidence: 99%

In vivo labeling with 2H2O reveals a human neutrophil lifespan of 5.4 days

Pillay¹,

Braber

Vrisekoop

et al. 2010

Blood

709

560

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IntroductionNeutrophils are indispensable for host defense. 1 In addition, these cells play a detrimental role in the pathogenesis of many acute and chronic inflammatory diseases. They can cause tissue damage through aspecific activation of their repertoire of antimicrobial mechanisms. Neutrophils also inform and shape subsequent immunity 2 and can prolong inflammation by release of cytokines 3 and chemokines. 4 There is an emerging concept that neutrophils directly influence adaptive immune responses through pathogen shuttling to draining lymph nodes, 5,6 antigen presentation, 7 and modulation of T helper 1/T helper 2 responses. 8 Along this line, neutrophils have been reported to be an important component of myeloid-derived suppressor cells mediating lymphocyte suppression in various experimental models of acute 9 and chronic inflammation. 10 Targeting neutrophils in disease has mainly been focused on limiting their damaging capacity or directing their cytotoxic machinery to tumors. 11 Their immune modulatory functions have received little attention as potential targets in inflammatory diseases. This may at least in part be due to the current paradigm that these functions are of limited importance because of the generally accepted short circulatory half-life of neutrophils. Neutrophil lifespans have mainly been assessed by determination of ex vivo lifespans in culture (Ͻ 24 hours) and by transfer studies of ex vivo-manipulated neutrophils. The latter studies showed an estimated circulating half-life of approximately 8 hours in humans. 12 Ex vivo manipulation has been shown to have dramatic effects on neutrophil redistribution in vivo. 13 In mice, half-lives of 8 to 10 hours were reported when neutrophils were labeled in vivo. 14 In contrast, ex vivo labeling in mice showed that after transfer 90% of labeled neutrophils were cleared from the circulation within 4 hours, resulting in a half-life of less than 1.5 hours. 15 These differences between in vivo and ex vivo labeling strengthen our hypothesis that neutrophil transfer experiments may lead to an underestimation of neutrophil lifespan. The activation during ex vivo manipulation has probably led to retention in the lungs, 16 liver, spleen, and bone marrow (BM), 15 which may drastically reduce their circulatory half-life. To circumvent the complications introduced by ex vivo manipulation, we labeled the neutrophil pool in vivo in healthy mice and humans by administration of 2 H 2 O in drinking water. Acquisition of label and appearance of labeled neutrophils in the circulation is characterized by (1) the rate of division in the mitotic pool (MP) in the BM, (2) the transit time of newly formed neutrophils through the postmitotic pool (PMP) in the BM, and (3) the delay in mobilization of neutrophils from the PMP to the blood. With the use of a combination of gas chromatography and mass spectrometry the fraction of 2 H-labeled adenosine in the DNA of the proliferating neutrophil pool was measured, and the kinetics of the neutrophil pool was determined. Study des...

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Measurement of Neutrophil Pulmonary Transit Time in Humans.

Cited by 4 publications

References 0 publications

What's your age again? Determination of human neutrophil half-lives revisited

What's your age again? Determination of human neutrophil half-lives revisited

Immune suppression by neutrophils and granulocytic myeloid-derived suppressor cells: similarities and differences

In vivo labeling with 2H2O reveals a human neutrophil lifespan of 5.4 days

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