Measurement of serum steroid profiles by HPLC-tandem mass spectrometry

Olisov, Dmitrii A.; Lee, Kyung-Hoon; Jun, Sun Hee; Song, Sang Hoon; Kim, Jae Hyun; Lee, Young Ah; Shin, Choong Ho; Song, Jae-Joon

doi:10.1016/j.jchromb.2019.04.001

Cited by 21 publications

(16 citation statements)

References 28 publications

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“…Other techniques, such as solid phase extraction (SPE) using columns, can be employed on their own or after LLE or protein precipitation using organic solvents [106]. LLE and SPE using reversed phase sorbents are ideal for extracting low abundant steroids [107]. The LLE method is appreciated for its speed and simplicity, while the SPE method can be semi-automated using 96well plates for instance [106].…”

Section: Methodologiesmentioning

confidence: 99%

“…On the other hand, derivatisation renders steroids more easily ionisable, thus, increasing the overall sensitivity that can be achieved [109]. Over the last ten years or so, GC-MS/MS has gradually been replaced by LC-MS/MS for the study of metabolites, including steroids [107]. The preferred use of LC reflects an improvement in the separation performance of these chromatographic systems [106].…”

Section: Methodologiesmentioning

confidence: 99%

“…Similarly, the LOQ concentration is the lowest concentration for which quantification makes sense. There are other parameters studied, such as the LOD ("limit of detection"), which is especially interesting to determine in the case of qualitative studies [107]. This is the lowest concentration at which a compound can be detected (S/N > 3) [104].…”

Section: Methodologiesmentioning

confidence: 99%

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Six Decades of Research on Human Fetal Gonadal Steroids

Connan-Perrot

Léger

Lelandais

et al. 2021

IJMS

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Human fetal gonads acquire endocrine steroidogenic capabilities early during their differentiation. Genetic studies show that this endocrine function plays a central role in the sexually dimorphic development of the external genitalia during fetal development. When this endocrine function is dysregulated, congenital malformations and pathologies are the result. In this review, we explain how the current knowledge of steroidogenesis in human fetal gonads has benefited from both the technological advances in steroid measurements and the assembly of detailed knowledge of steroidogenesis machinery and its expression in human fetal gonads. We summarise how the conversion of radiolabelled steroid precursors, antibody-based assays, mass spectrometry, ultrastructural studies, and the in situ labelling of proteins and mRNA have all provided complementary information. In this review, our discussion goes beyond the debate on recommendations concerning the best choice between the different available technologies, and their degrees of reproducibility and sensitivity. The available technologies and techniques can be used for different purposes and, as long as all quality controls are rigorously employed, the question is how to maximise the generation of robust, reproducible data on steroid hormones and their crucial roles in human fetal development and subsequent functions.

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Section: Methodologiesmentioning

confidence: 99%

Section: Methodologiesmentioning

confidence: 99%

Section: Methodologiesmentioning

confidence: 99%

See 1 more Smart Citation

Six Decades of Research on Human Fetal Gonadal Steroids

Connan-Perrot

Léger

Lelandais

et al. 2021

IJMS

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“…Data were evaluated by Quan/Qual Browser Xcalibur software, version 4.0. Identification of steroid compounds in samples was based on their retention times relative to those of the authentic standards and on mass spectral data (accurate mass determination generating elemental composition and fragmentation patterns of a molecular ion) obtained by LC-MS/MS, which were compared with those described in previous studies [47,48]. Quantitative analysis was performed on the basis of calibration curves.…”

Section: Quantification Of Steroids Using Ms Analysismentioning

confidence: 99%

The Gut Microbiota Affects Corticosterone Production in the Murine Small Intestine

Ergang

Vagnerová

Hermanová

et al. 2021

IJMS

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Glucocorticoids (GCs) are hormones that are released in response to stressors and exhibit many activities, including immunomodulatory and anti-inflammatory activities. They are primarily synthesized in the adrenal gland but are also produced in peripheral tissues via regeneration of adrenal 11-oxo metabolites or by de novo synthesis from cholesterol. The present study investigated the influence of the microbiota on de novo steroidogenesis and regeneration of corticosterone in the intestine of germ-free (GF) and specific pathogen-free mice challenged with a physical stressor (anti-CD3 antibody i.p. injection). In the small intestine, acute immune stress resulted in increased mRNA levels of the proinflammatory cytokines IL1β, IL6 and Tnfα and genes involved in de novo steroidogenesis (Stard3 and Cyp11a1), as well as in regeneration of active GCs from their 11-oxo metabolites (Hsd11b1). GF mice showed a generally reduced transcriptional response to immune stress, which was accompanied by decreased intestinal corticosterone production and reduced expression of the GC-sensitive marker Fkbp5. In contrast, the interaction between stress and the microbiota was not detected at the level of plasma corticosterone or the transcriptional response of adrenal steroidogenic enzymes. The results indicate a differential immune stress-induced intestinal response to proinflammatory stimuli and local corticosterone production driven by the gut microbiota.

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“…The investigators at NIST have revealed the photosensitivity of progesterone, which must be considered during sample handling, which in their case includes a pH adjustment (pH 9.8) followed by hexane LLE (Tai et al, 2006). The m / z transition used for +ESI multiple reaction monitoring (MRM) (314.8 ➔ 97.1) is the same as or similar to what is used in many other progesterone assays moving forward (Desai, Harwood, & Handelsman, 2019; Farke et al, 2011; Gaikwad, 2013; Galligan et al, 2018; Gao et al, 2015; Genangeli et al, 2017; Gibson, Bucknall, Golebiowski, & Stapleton, 2019; Gomez‐Gomez et al, 2020; Häkkinen et al, 2018; Higashi et al, 2011; Lee, Lee, Hong, Chung, & Choi, 2016; McCulloch & Robb, 2017; Olisov et al, 2019; Schiffer, Adaway, Baranowski, Arlt, & Keevil, 2018; Surowiec, Koc, Antti, Wikström, & Moritz, 2011; Tai et al, 2006; Voegel et al, 2018; Zhou & Cai, 2020).…”

Section: Progesterone Bioanalysismentioning

confidence: 99%

Liquid chromatography–mass spectrometry applications for quantification of endogenous sex hormones

Gravitte

Archibald

Cobble

et al. 2020

Biomedical Chromatography

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Liquid chromatography, coupled with tandem mass spectrometry, presents a powerful tool for the quantification of the sex steroid hormones 17-β estradiol, progesterone and testosterone from biological matrices. The importance of accurate quantification with these hormones, even at endogenous levels, has evolved with our understanding of the role these regulators play in human development, fertility and disease risk and manifestation. Routine monitoring of these analytes can be accomplished by immunoassay techniques, which face limitations on specificity and sensitivity, or using gas chromatography-mass spectrometry. LC-MS/MS is growing in capability and acceptance for clinically relevant quantification of sex steroid hormones in biological matrices and is able to overcome many of the limitations of immunoassays. Analyte specificity has improved through the use of novel derivatizing agents, and sensitivity has been refined through the use of high-resolution chromatography and mass spectrometric technology. This review highlights these innovations, among others, in LC-MS/MS steroid hormone analysis captured in the literature over the last decade.

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Measurement of serum steroid profiles by HPLC-tandem mass spectrometry

Cited by 21 publications

References 28 publications

Six Decades of Research on Human Fetal Gonadal Steroids

Six Decades of Research on Human Fetal Gonadal Steroids

The Gut Microbiota Affects Corticosterone Production in the Murine Small Intestine

Liquid chromatography–mass spectrometry applications for quantification of endogenous sex hormones

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