Measurements of ? potentials of particulate biomaterials in protein-rich hyaluronan solution with changes in pH and protein constituents

Abstract

This study was undertaken to determine the zeta potentials of particulate biomaterials in three types of protein-rich hyaluronan solution with changes in pH; a microelectrophoretic method was used. For the purpose of determining the pH value of synovial fluid in various inflammatory conditions, we collected synovial fluid samples from joints with osteoarthritis (OA), rheumatoid arthritis (RA), and those undergoing revisions arthroplasties. The mean values of the pH in the synovial fluid from joints with OA, RA, and revision arthroplasty were shown to be 7.9, 7.5, and 8.1, respectively. The pH-zeta potential curves obtained differed, depending on the biomaterial and the medium. Addition of gamma-globulin to the medium reduced the absolute value of the zeta potentials of some of the biomaterials. The findings of this study suggest that the electrophoretic behaviors of the particulate biomaterials tested in this study are affected by the protein constituents of and pH changes in protein-rich synovial fluid. The values we obtained will be useful as reference standards and will also aid in the study of the surface phenomena of biomaterials.

Alginate-based microcapsules for immunoisolation of pancreatic islets

“…The zeta-potential is a measure for the electrical charge of the surface and a predictive value for the interfacial reactions between the biomaterial and the surrounding tissue [87][88][89]. When comparing the zetapotential of capsules prepared of intermediate-G and high-G alginate-PLL capsules under a physiological pH value of 7, we found no differences in zeta-potentials and thus in electrical charge distributions.…”

Effect of thermal treatment on bioactive glass microstructure, corrosion behavior, ? potential, and protein adsorption

“…Lu et al 32 showed that the addition of fibronectin to SBF dramatically lowered bioactive glass surface negativity owing to protein adsorption. In another study, Kitano et al 33 reported that addition of ␥-globulin to the medium reduced the negative value of the ZP of hydroxyapatite and polycrystalline alumina ceramic. However, the effect of surface charge on the quantity of protein adsorbed to the materials was not reported.…”

The effect of Gd@C82(OH)22 nanoparticles on the release of Th1/Th2 cytokines and induction of TNF-α mediated cellular immunity

“…The zeta potential is a measure from the electrical charge of the surface and a predictive value for the interfacial reactions between the biomaterial and the surrounding tissue [22,23]. To observe the nanoparticle stability in physiological media, the size distribution of Gd@C 82 (OH) 22 nanoparticles kept in the complete medium for 1 month at 4 C was measured using DLS.…”