Measurements of the rate of production of bacteria in the rumen of buffalo calves

Singh, Umesh; Verma, D. N.; Varma, Ajit; Ranjhan, S. K.

doi:10.1017/s0021859600046931

Cited by 12 publications

(8 citation statements)

References 7 publications

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“…The production varied from 124 to 166 g/day in animals offered green maize, and from 219 to 266 g/day when cow pea was fed. In our previous experiments, in which a concentrate ration together with chopped wheat straw was fed to the animals, a cell yield of 137 g/kg DOM was obtained (Singh et al 1974c). This suggests that the efficiency of protein synthesis by the rumen bacteria varies depending upon the composition of feed and microbial community.…”

Section: Resultsmentioning

confidence: 91%

“…In the procedure described earlier (Singh et al 1974c) the mixed bacterial population of the rumen was tagged with radioactive carbon or sulphur and was replaced into the rumen to estimate the dilution rate and eventually the production of ruminal bacteria. The method was based on the assumption that the proportion of individual bacterial species in the rumen in that particular diet /kg DOM) 80-6 91-9 92-5 88-3 3-88 9 2 0 89-3 95-6 92-3 1-82 100-7 103-4 105-8 103-3 1-49 99-8 100-6 104-8 101-8 1-55 to C i does not change with time and the sample of rumen contents drawn for the estimation of specific radioactivity will contain all the species of rumen bacteria in the same proportion, and the fractional turnover rates are the average of various species of bacteria and presumably the turnover rates are similar for different species.…”

Section: Resultsmentioning

confidence: 99%

“…Ruminal bacteria from the experimental animals were labelled with sulphur-35 by an in vitro incubation as described earlier (Singh et al 1974c).…”

Section: Preparation Of Labelled Mixed Bacteriamentioning

confidence: 99%

“…Samples from the rumen were drawn at various time intervals up to 9 h, from four different sites with the help of specially built probes as described by Chaturvedi, . The rumen fluid samples (35 ml) were transferred to a cold vessel and were processed immediately (Singh et at. 1974c).…”

Section: Preparation Of Labelled S Bo Vismentioning

confidence: 99%

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Comparison of the production rate of bacteria in the rumen of buffalo calves estimated by using labelledStreptococcus bovisand mixed ruminal bacterial cells

Verma¹,

Singh²,

Srivastava³

et al. 1976

J. Agric. Sci.

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The rate of production of bacteria in the rumen of buffalo calves kept on two rations was measured using 14 C labelled Streptococcus bovis and 35 S whole ruminal bacterial cells. The animals received daily either 15-20 kg green maize or 25-30 kg green cow pea in 12 equal amounts at 2-h intervals. The bacterial cells from the rumen of animals maintained on the same diet were tagged with 36 S by in vitro incubation in the presence of 35 S-sodium sulphate. Similarly Streptococcus bovis of rumen origin was grown in the presence of U-14 C DL-leucine. The ceJls were injected into the rumen in a single dose. The dilution of the specific radioactivity of bacterial cells in the rumen with time was taken for calculation of the turnover time and rate of production of bacteria.The average production rates of bacteria were 88-3 ± 3-88 and 92-3 ± 1-82 g/kg digestible organic matter and 101-8 + 1-55 and 103-3 + 1-49 g/kg digestible organic matter in animals fed green maize and cow pea, when estimated by using mixed rumen whole bacterial cells and Streptococcus bovis respectively. There was no significant difference in the rate of bacteria production when estimated by either method.t n e r u m e n ( 14 Q Streptococcus bovis was used to estimate bacteria production and comparison made Microbes in the rumen degrade a large proportion of the bacterial growth of the rumen obtained either of dietary proteins and utilize some of the degrada-by injecting labelled mixed rumen bacterial cells tion products for their own protein synthesis. or pure cultures of Streptococcus bovis of rumen These microbes can also make use of non-protein origin. nitrogen (NPN) compounds and can upgrade the EXPERIMENTAL PROCEDURE dietary proteins of low biological value into microbial proteins which are of good biological value Animals and feeding regime (Bergen, Purser & Cline, 1967). Whereas the utilizaThree male Murrah buffalo (Bos bubalis) of about tion of NPN by the rumen microbes is very advan-2 years of age were used in these experiments. All tageous in animal feeding, the degradation of the the animals were fitted with permanent cannulae dietary proteins of high biological value is a dis-in rumen fistulae. In the first set of experiments advantage. Therefore, it is important to know the each animal was given 15-20 kg of green chopped extent of microbial growth in a particular dietary maize daily whereas in the second experiment regime in order to assess its effectiveness for 25-30 kg cow pea was fed to each animal. The production.residue was measured to assess intake. The calves In our previous communications an experi-were kept on a pre-experimental feeding period for mental approach was described for the measure-5 weeks during which they received their ration ments of bacterial and protozoal production rates once daily and thereafter they received their daily in the rumen using 36 S and 14 C isotope dilution ration in 12 equal amounts at 2-h intervals for a techniques (Singh et al. 1973, 1974a, b and c). In period of 3 weeks. The residue, if any, le...

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Section: Resultsmentioning

confidence: 91%

Section: Resultsmentioning

confidence: 99%

“…Ruminal bacteria from the experimental animals were labelled with sulphur-35 by an in vitro incubation as described earlier (Singh et al 1974c).…”

Section: Preparation Of Labelled Mixed Bacteriamentioning

confidence: 99%

Section: Preparation Of Labelled S Bo Vismentioning

confidence: 99%

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Comparison of the production rate of bacteria in the rumen of buffalo calves estimated by using labelledStreptococcus bovisand mixed ruminal bacterial cells

Verma¹,

Singh²,

Srivastava³

et al. 1976

J. Agric. Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Alternatively, an injection of a known dose of rumen microbes, labelled with 35 S by an in vitro incubation procedure, may be made t Present address: Department of Animal Science, University of Alberta, Edmonton, Canada T6G 2E3. (Singh et al 1974). In all three methods, the rate of decline of the specific radioactivity of microbes must be estimated, entailing an appreciable number of analyses.…”

Section: Introductionmentioning

confidence: 99%

In vivo measurement of rumen microbial growth using ³⁵S

Kennedy¹,

Lindsay²,

Milligan³

1980

J. Agric. Sci.

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A method for the measurement of the net rate of synthesis of rumen microbes, entailing separate infusions of 35 S-sulphate into the blood and rumen, is described and compared with estimates derived using digesta flow markers. Measurements were made on seven occasions with sheep and on eight occasions with cattle given chopped hay or pelleted hay diets at intervals of 60 or 90 min. Additional measurements were made on two sheep and two cattle given lucerne hay at intervals of 6 or 12 h. Estimates of microbial synthesis using the proposed method for animals fed at intervals of 60 or 90 min were 0-95-1-23 (mean 1-05) that derived using flow markers. For animals fed at intervals of 6 or 12 h the ratio was more variable, 0-91-1-35 (mean 1-17). The agreement between methods tended to be poor in animals with a low rate of irreversible loss of serum sulphate. The proposed method can be accomplished with a low analytical load, and appears to have potential for application in animals fitted with rumen fistulae only, and fed at infrequent intervals.

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Possibility of using131I-albumin as a marker for the estimations of microbial protein synthesis rates in the rumen

et al. 1977

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Total microbial protein synthesis rates in the rumen of buffaloes were estimated by isotope dilution technique, using 131I-albumin treated with tannic acid as a marker. The animals were fed groundnut cake treated with formaldehyde to meet 50% of their digestible crude protein (DCP) requirement and 2.5% urea molasses mixture was given to meet the remaining requirement of DCP. Wheat straw was fed as the basal roughage. The total average microbial protein synthesis was 58.14 g/day.

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Measurements of the rate of production of bacteria in the rumen of buffalo calves

Cited by 12 publications

References 7 publications

Comparison of the production rate of bacteria in the rumen of buffalo calves estimated by using labelledStreptococcus bovisand mixed ruminal bacterial cells

Comparison of the production rate of bacteria in the rumen of buffalo calves estimated by using labelledStreptococcus bovisand mixed ruminal bacterial cells

In vivo measurement of rumen microbial growth using ³⁵S

Possibility of using131I-albumin as a marker for the estimations of microbial protein synthesis rates in the rumen

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Measurements of the rate of production of bacteria in the rumen of buffalo calves

Cited by 12 publications

References 7 publications

Comparison of the production rate of bacteria in the rumen of buffalo calves estimated by using labelledStreptococcus bovisand mixed ruminal bacterial cells

Comparison of the production rate of bacteria in the rumen of buffalo calves estimated by using labelledStreptococcus bovisand mixed ruminal bacterial cells

In vivo measurement of rumen microbial growth using 35S

Possibility of using131I-albumin as a marker for the estimations of microbial protein synthesis rates in the rumen

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In vivo measurement of rumen microbial growth using ³⁵S