Measuring CO2 and HCO3− permeabilities of isolated chloroplasts using a MIMS-18O approach

Tolleter, Dimitri; Chochois, Vincent; Poiré, Richard; Price, G. Dean; Badger, Murray R.

doi:10.1093/jxb/erx188

Cited by 30 publications

(23 citation statements)

References 31 publications

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“…If the changes in g m with respect to C i were attributed solely to the large permeability of the chloroplast envelope to HCO 3 − , the increase of g m with the decrease in C i would be expected. Moreover, a recent study showed the permeability of HCO 3 − to be 1.0 × 10 −8 m s −1 in A. thaliana (Tolleter, Chochois, Poire, Price, & Badger, ). Thus, the hypothesis based on the high permeability of the chloroplast envelope to HCO 3 − (Heber & Purczeld, ; Tholen & Zhu, ) may not be vital.…”

Section: Discussionmentioning

confidence: 99%

Effects of instantaneous and growth CO₂ levels and abscisic acid on stomatal and mesophyll conductances

Mizokami

Noguchi

Kojima

et al. 2018

Plant Cell & Environment

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C3 photosynthesis is often limited by CO2 diffusivity or stomatal (gs) and mesophyll (gm) conductances. To characterize effects of stomatal closure induced by either high CO2 or abscisic acid (ABA) application on gm, we examined gs and gm in the wild type (Col‐0) and ost1 and slac1‐2 mutants of Arabidopsis thaliana grown at 390 or 780 μmol mol−1 CO2. Stomata of these mutants were reported to be insensitive to both high CO2 and ABA. When the ambient CO2 increased instantaneously, gm decreased in all these plants, whereas gs in ost1 and slac1‐2 was unchanged. Therefore, the decrease in gm in response to high CO2 occurred irrespective of the responses of gs. gm was mainly determined by the instantaneous CO2 concentration during the measurement and not markedly by the CO2 concentration during the growth. Exogenous application of ABA to Col‐0 caused the decrease in the intercellular CO2 concentration (Ci). With the decrease in Ci, gm did not increase but decreased, indicating that the response of gm to CO2 and that to ABA are differently regulated and that ABA content in the leaves plays an important role in the regulation of gm.

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Section: Discussionmentioning

confidence: 99%

Effects of instantaneous and growth CO₂ levels and abscisic acid on stomatal and mesophyll conductances

Mizokami

Noguchi

Kojima

et al. 2018

Plant Cell & Environment

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“…Therefore, in plants with their roots exposed to HCO 3 − , the proportion of HCO 3 − over CO 2 during radial transport of C inorg from soil to the stele and within the xylem sap up to the leaves may be considerably lower than in the soil solution surrounding the plant roots. The direct use of root-derived HCO 3 − by CA in chloroplasts to supply CO 2 for Rubisco is unlikely when taking into account the low chloroplast permeability of HCO 3 − (1 × 10 −8 m s −1 ) in comparison to CO 2 (range from 2.3 × 10 −4 to 8 × 10 −4 m s −1 ), which was recently shown by mass inlet mass spectrometry (MIMS) using 18 O labelled C inorg [ 131 ].…”

Section: Bicarbonate Transportmentioning

confidence: 99%

Transport and Use of Bicarbonate in Plants: Current Knowledge and Challenges Ahead

Poschenrieder

Fernández

Rubio

et al. 2018

IJMS

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Bicarbonate plays a fundamental role in the cell pH status in all organisms. In autotrophs, HCO3− may further contribute to carbon concentration mechanisms (CCM). This is especially relevant in the CO2-poor habitats of cyanobacteria, aquatic microalgae, and macrophytes. Photosynthesis of terrestrial plants can also benefit from CCM as evidenced by the evolution of C4 and Crassulacean Acid Metabolism (CAM). The presence of HCO3− in all organisms leads to more questions regarding the mechanisms of uptake and membrane transport in these different biological systems. This review aims to provide an overview of the transport and metabolic processes related to HCO3− in microalgae, macroalgae, seagrasses, and terrestrial plants. HCO3− transport in cyanobacteria and human cells is much better documented and is included for comparison. We further comment on the metabolic roles of HCO3− in plants by focusing on the diversity and functions of carbonic anhydrases and PEP carboxylases as well as on the signaling role of CO2/HCO3− in stomatal guard cells. Plant responses to excess soil HCO3− is briefly addressed. In conclusion, there are still considerable gaps in our knowledge of HCO3− uptake and transport in plants that hamper the development of breeding strategies for both more efficient CCM and better HCO3− tolerance in crop plants.

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“…Note that in some cases like a concomitant use of 18 O-labeled O 2 , the presence of 18 O and C 16 O 2 in the ion source of the mass spectrometer can spontaneously generate C 18 O 16 O which needs to be corrected ( Cournac et al., 1993 ). In vivo , a CA isotope exchange assay measures the global contribution of all CAs present in the biological sample with an additional effect due to diffusion/transport of C i through the membranes ( Sültemeyer and Rinast, 1996 ; Tolleter et al., 2017 ). When a periplasmic CA is present, as it is the case in C. reinhardtii cells with an active CCM, its activity dominates the exchange kinetics ( Sültemeyer and Rinast, 1996 ).…”

Section: Mims Usage In Algal Researchmentioning

confidence: 99%

Membrane Inlet Mass Spectrometry: A Powerful Tool for Algal Research

Burlacot

Li‐Beisson

et al. 2020

Front. Plant Sci.

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Since the first great oxygenation event, photosynthetic microorganisms have continuously shaped the Earth's atmosphere. Studying biological mechanisms involved in the interaction between microalgae and cyanobacteria with the Earth's atmosphere requires the monitoring of gas exchange. Membrane inlet mass spectrometry (MIMS) has been developed in the early 1960s to study gas exchange mechanisms of photosynthetic cells. It has since played an important role in investigating various cellular processes that involve gaseous compounds (O 2 , CO 2 , NO, or H 2) and in characterizing enzymatic activities in vitro or in vivo. With the development of affordable mass spectrometers, MIMS is gaining wide popularity and is now used by an increasing number of laboratories. However, it still requires an important theory and practical considerations to be used. Here, we provide a practical guide describing the current technical basis of a MIMS setup and the general principles of data processing. We further review how MIMS can be used to study various aspects of algal research and discuss how MIMS will be useful in addressing future scientific challenges.

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Measuring CO2 and HCO3− permeabilities of isolated chloroplasts using a MIMS-18O approach

Abstract: New approach to determine the inorganic carbon permeability across the chloroplast envelope, and to measure the role of aquaporin Pip1,2 on CO2 permeability.

Cited by 30 publications

References 31 publications

Effects of instantaneous and growth CO₂ levels and abscisic acid on stomatal and mesophyll conductances

Effects of instantaneous and growth CO₂ levels and abscisic acid on stomatal and mesophyll conductances

Transport and Use of Bicarbonate in Plants: Current Knowledge and Challenges Ahead

Membrane Inlet Mass Spectrometry: A Powerful Tool for Algal Research

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Measuring CO2 and HCO3− permeabilities of isolated chloroplasts using a MIMS-18O approach

Abstract: New approach to determine the inorganic carbon permeability across the chloroplast envelope, and to measure the role of aquaporin Pip1,2 on CO2 permeability.

Cited by 30 publications

References 31 publications

Effects of instantaneous and growth CO2 levels and abscisic acid on stomatal and mesophyll conductances

Effects of instantaneous and growth CO2 levels and abscisic acid on stomatal and mesophyll conductances

Transport and Use of Bicarbonate in Plants: Current Knowledge and Challenges Ahead

Membrane Inlet Mass Spectrometry: A Powerful Tool for Algal Research

Contact Info

Product

Resources

About

Effects of instantaneous and growth CO₂ levels and abscisic acid on stomatal and mesophyll conductances

Effects of instantaneous and growth CO₂ levels and abscisic acid on stomatal and mesophyll conductances