Measuring dissolved oxygen to track erythroid differentiation of hematopoietic progenitor cells in culture

Browne, Susan M.; Daud, Hasbullah; Murphy, William G.; Al‐Rubeai, Mohamed

doi:10.1016/j.jbiotec.2014.07.433

Cited by 9 publications

(8 citation statements)

References 21 publications

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“…As observed by [37], at some point of the erythroid differentiation process, energy demand increased, and glycolysis as well as OXPHOS were enhanced. Moreover a peak of cell respiration was reported during erythroid differentiation at the high proliferation phase [46]. It has been shown that erythroid differentiation initiation indeed was characterized by an initial increase of the proliferation rate [16, 47].…”

Section: Discussionmentioning

confidence: 99%

Erythroid differentiation displays a peak of energy consumption concomitant with glycolytic metabolism rearrangements

et al. 2019

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Our previous single-cell based gene expression analysis pointed out significant variations of LDHA level during erythroid differentiation. Deeper investigations highlighted that a metabolic switch occurred along differentiation of erythroid cells. More precisely we showed that self-renewing progenitors relied mostly upon lactate-productive glycolysis, and required LDHA activity, whereas differentiating cells, mainly involved mitochondrial oxidative phosphorylation (OXPHOS). These metabolic rearrangements were coming along with a particular temporary event, occurring within the first 24h of erythroid differentiation. The activity of glycolytic metabolism and OXPHOS rose jointly with oxgene consumption dedicated to ATP production at 12-24h of the differentiation process before lactate-productive glycolysis sharply fall down and energy needs decline. Finally, we demonstrated that the metabolic switch mediated through LDHA drop and OXPHOS upkeep might be necessary for erythroid differentiation. We also discuss the possibility that metabolism, gene expression and epigenetics could act together in a circular manner as a driving force for differentiation.

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Section: Discussionmentioning

confidence: 99%

Erythroid differentiation displays a peak of energy consumption concomitant with glycolytic metabolism rearrangements

et al. 2019

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“…Moreover a peak of cell respiration was reported during erythroid differentiation at the 475 high proliferation phase [46]. It has been shown that erythroid differentiation initiation 476 indeed was characterized by an initial increase of the proliferation rate [16,47].…”

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confidence: 99%

“…Finally, LDHA role in erythroid progenitors self-renewal was assayed 45 using known molecular inhibitors and the CRISPR/Cas9 knock-out technology. Then, 46 to investigate whether LDHA downregulation could be responsible for metabolic state 47 changes in erythroid progenitors, we measured MMP of self-renewing cells following 48 inhibition of LDHA activity. Finally, we assessed whether or not the metabolic shift 49 toward OXPHOS might be necessary for T2EC differentiation.…”

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confidence: 99%

Erythroid differentiation displays a peak of energy consumption concomitant with glycolytic metabolism rearrangements

Angélique

Vallin

Romestaing

et al. 2019

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Author summarySingle-cell based gene expression data from one of our previous publication pointed out significant variations of LDHA level, an important metabolism player, during erythroid differentiation. Deeper investigations highlighted that a metabolic switch occurred along differentiation of erythroid cells as previously emphasized in stem cell differentiation. More precisely our finding showed that self-renewing progenitor cells relied mostly upon a glycolytic, lactate-productive, metabolism and required LDHA activity, whereas differentiating cells, mainly involved the aerobic mitochondrial oxidative phosphorylation (OXPHOS). However our careful kinetic study demonstrated that these metabolic rearrangements were coming along with a particular temporary event, occurring within the first 24h of erythroid differentiation. The activity of glycolytic metabolism and OXPHOS rose jointly with ATP production at 12-24h of the differentiation process before lactate-productive glycolysis sharply fall down and energy needs decline. Finally, our results showed that the metabolic switch mediated through LDHA drop and OXPHOS upkeep might be necessary for erythroid differentiation. We also discuss the possibility that metabolism, gene expression and epigenetics could act together in a circular manner as a driving force for differentiation. Introduction 1Metabolism is a biological process mostly involved in energy consumption, production 2 and distribution, which is essential for cell functions and survival.3 An emerging theme is the possible causal involvement of metabolic changes during a 4 differentiation process. In particular, glycolysis was shown to be characteristic of 5 self-renewing stem cells and of different types of progenitors, such as neuronal and 6 hematopoietic progenitor cells [1,2]. It has been demonstrated that stem cells tend to 7 switch from glycolytic metabolism toward mitochondrial oxidative phosphorylation 8 (OXPHOS) while they differentiate [3][4][5]. Accordingly, it was suggested that a balance 9 between glycolysis and OXPHOS metabolism could somehow guide the choice between 10 self-renewal and differentiation in stem cells fate [6]. It has also been demonstrated that 11 preventing the shutting down of the glycolytic pathway was detrimental for neuronal 12 differentiation [4]. Otherwise, regarding somatic cells reprogramming into induced 13 pluripotent stem cells, it has been reported that pluripotency induction required the 14 concomitant upregulation of glycolytic metabolism and downregulation of OXPHOS [7]. 15 Regarding erythroid differentiation, progenitors depend on glycolysis and present a 16 Warburg-like profile as they display a high proliferation rate and produce an abundant 17 amount of lactate [8]. However, few is known about glucose metabolism behavior during 18 erythroid differentiation, when compared to erythroid progenitors self-renewal. Mature 19 mammals erythrocytes were shown to rely upon lactate dehydrogenase to fulfill their 20 functions, depending therefore upon the glycolytic p...

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“…Previous studies have demonstrated a distinct metabolic profile during during HSPC differentiation into RBCs [28][29][30]. Generally, proliferative cells with a very high growth rate rely on glycolysis for energy, while differentiating cells switch to more efficient energy production through OXPHOS [28,31].…”

Section: Resultsmentioning

confidence: 99%

Metabolic profiling of hematopoietic stem and progenitor cells during proliferation and differentiation into red blood cells

et al. 2016

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Measuring dissolved oxygen to track erythroid differentiation of hematopoietic progenitor cells in culture

Cited by 9 publications

References 21 publications

Erythroid differentiation displays a peak of energy consumption concomitant with glycolytic metabolism rearrangements

Erythroid differentiation displays a peak of energy consumption concomitant with glycolytic metabolism rearrangements

Erythroid differentiation displays a peak of energy consumption concomitant with glycolytic metabolism rearrangements

Metabolic profiling of hematopoietic stem and progenitor cells during proliferation and differentiation into red blood cells

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