Measuring fecal progestogens as a tool to monitor reproductive activity in captive female bottlenose dolphins (Tursiops truncatus)

Biancani, Barbara; Dalt, Laura Da; Lacave, G.; Romagnoli, Stefano; Gabai, Gianfranco

doi:10.1016/j.theriogenology.2009.07.025

Cited by 30 publications

(20 citation statements)

References 28 publications

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“…Regu‐mate has been used in a variety of cetacean species for both estrous synchronization and reversible contraception without adverse effects reported (Robeck et al 2001, AZA CAG 2004). It promotes suppression of the release of gonadotropin and the blocking of ovarian follicular development and estrus (Biancani et al 2009). F6 and F8 were observed to have an increasing thyroid volume following treatment with oral altrenogest.…”

Section: Discussionmentioning

confidence: 99%

Sonographic evaluation of thyroid morphology during different reproductive events in female Indo‐Pacific bottlenose dolphins, Tursiops aduncus

Kot¹,

Ying²,

Brook³

et al. 2011

Marine Mammal Science

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Thyroid morphology and function are likely affected by the cyclic hormonal environment during different reproductive events in females. The present study was undertaken to evaluate the variation of thyroid morphology at different reproductive events (anestrus, estrus, lactation, and pregnancy) in a captive group of Indo‐Pacific bottlenose dolphins (Tursiops aduncus) measured using sonography. Sonographic examinations of the thyroid gland and ovaries in nine sexually mature female subjects were performed weekly for 2.5 yr. A generalized linear mixed model was used to estimate the effects of the reproductive events for thyroid volume. Reproductive event was found to be a significant predictor for thyroid volume measurement and significant variation in thyroid volume was found between different reproductive events. A significantly larger thyroid volume in lactating females was observed when compared with estrous and anestrous females, possibly due to the high energy requirements and milk production during lactation. Taken together, thyroid volume variation during different reproductive events in female dolphins should be considered so as to obtain a diagnostically meaningful assessment when conducting routine examinations.

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Section: Discussionmentioning

confidence: 99%

Sonographic evaluation of thyroid morphology during different reproductive events in female Indo‐Pacific bottlenose dolphins, Tursiops aduncus

Kot¹,

Ying²,

Brook³

et al. 2011

Marine Mammal Science

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“…The RIA already used to measure estrogens and progestogens in fecal extracts from bottlenose dolphins (Biancani et al 2009) performed very well in measuring fecal sex steroid concentrations in female cheetahs. The parallelism was good for both the fecal estrogen and progestogen assays, and both assays could detect the hormone across a wide range of dilutions.…”

Section: Discussionmentioning

confidence: 99%

“…Sexual steroid concentrations were analyzed by solid-phase microtiter RIAs as previously described in bottlenose dolphins (Biancani et al, 2009). Briefly, 96-well microtiter plates (Perkin-Elmer Life and Analytical Sciences, Shelton, CT, USA) were coated with a goat anti-rabbit γ-globulin serum diluted 1:1000 in 0.15 mM sodium acetate buffer pH 9 and incubated overnight at 4 C. The plates were carefully…”

Section: Estrogen and Progestogen Radioimmunoassays (Ria)mentioning

confidence: 99%

“…A non-invasive monitoring of endocrine status can be performed by measurement of steroid metabolites in urine and/or feces in different species (Biancani et al, 2009;Lasley and Kirkpatrick, 1991). In felids, because sexual steroids are excreted mainly in feces, and urine is voided by spraying and hard to collect in the field, the extraction from fecal samples is the elective method (Graham et al, 1995;Shille et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

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One year daily changes in fecal sexual steroids of two captive female cheetahs ( Acinonyx jubatus ) in Italy

Vernocchi

Morselli

Faustini

et al. 2018

Animal Reproduction Science

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Please cite this article as: Vernocchi V, Morselli MG, Faustini M, Gabai G, Da, Dalt L, Luvoni GC, One year daily changes in fecal sexual steroids of two captive female cheetahs (acinonyx jubatus) in italy, Animal Reproduction Science (2010), https://doi.org/10. 1016/j.anireprosci.2018.01.016 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. in the two females, underlying throughout the year a more pronounced rhythmical ovarian estrogenic activity in Geijsha than in Duchessa. However, this statistical approach had a smoothing effect in depicting the hormonal patterns and the number of follicular phases might be lower than that revealed by the iterative method.In this study, RIA on wet feces performed very well to determine sexual steroid concentrations, and an ovarian activity interrupted by anestrous periods along the year in captive cheetahs co-housed in a small group was demonstrated.More information on estrous behavior of captive cheetahs were obtained in this study, but the effects of husbandry and management conditions on natural reproductive physiology of this species remain to elucidate.

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“…) or may be given voluntarily by trained captive animals (Biancani et al. ). The most commonly used test to measure hormone metabolites in cetacean faeces was RIA, although other tests may work as well (Table ).…”

Section: Biological Matricesmentioning

confidence: 99%

Biological matrices for sampling free‐ranging cetaceans and the implications of their use for reproductive endocrine monitoring

Mello

Oliveira

2016

Mammal Review

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Monitoring reproductive rates in wild cetaceans is a crucial step towards adequate management of their populations. Their reproductive hormone profiles can be of importance, especially when combined with behavioural or life history monitoring. Sampling biological matrices of wild, free‐ranging cetaceans can be a difficult task. The purposes of this review are: to describe briefly the different methods available for monitoring the reproductive endocrine parameters of wild and captive cetaceans, and to evaluate the feasibility of using various matrices – blood, urine, faeces, saliva, bubbler, muscle, ocular secretions, blow (respiratory vapour), milk, baleen, and earplug materials – to measure sex hormones in free‐ranging cetaceans. We performed a retrospective analysis of the measurement and sample collection methods and the most commonly used matrices for constructing reproductive hormone profiles. The PubMed and Google Scholar data bases were used, and scientific articles and book chapters published from 1980 to the present were considered. In the past, the most commonly used matrix for measuring hormones of free‐ranging cetaceans was blood. This has been changing since studying live whales became more common: researchers have dispensed with using blood samples from dead animals. Matrices such as blow, which can be obtained non‐invasively, and other matrices that can be obtained with minimal invasion, such as blubber, appear to be feasible options for monitoring the hormones of live, free‐ranging cetaceans.

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Measuring fecal progestogens as a tool to monitor reproductive activity in captive female bottlenose dolphins (Tursiops truncatus)

Cited by 30 publications

References 28 publications

Sonographic evaluation of thyroid morphology during different reproductive events in female Indo‐Pacific bottlenose dolphins, Tursiops aduncus

Sonographic evaluation of thyroid morphology during different reproductive events in female Indo‐Pacific bottlenose dolphins, Tursiops aduncus

One year daily changes in fecal sexual steroids of two captive female cheetahs ( Acinonyx jubatus ) in Italy

Biological matrices for sampling free‐ranging cetaceans and the implications of their use for reproductive endocrine monitoring

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