The acknowledge on air ions beneficial bioactivities, especially those negative charge, small size and oxygen composition rich atmospheres; may indicate local environmental quality, increase health tourism attractiveness, promote wellbeing and support therapies. Negative air ions (NAI) microclimates anomalies by natural mechanisms or controlled generations may indicate a natural therapeutic factor (NTF) to the health resort medicine, SPA and wellness tourism development. To identify these kind occurrences and their potential bioactivities, this study employs literature review concerning "air ions" definitions, classifications, genesis, environments, anomalies, NAI/cm 3 measurements, bioactivity range and healthy applications. Followed by sites selection, with comparative features at preferably traditional wellness or health tourism destinations; where "in situ" NAI concentrations are measured for further physical comparisons and likely healing indications at each place maximum NAI value observed. The case studies here are six Brazilian locations: 1. Itaipuland/PR (Cave SPA termal); 2a. Guarapari/ES (Areia Preta beach) y 2b. Guarapari/ES (Seacoast downtown); 3. Aguas de Lindoia/SP (Public balneary and emanatorium); 4. Foz do Iguaçu/PR (Cataratas Waterfalls Park); 5. Caldas de Cipó/BA (Urban public thermal pool); and 6. Piracicaba/SP (Riverside urban forest Park). Mainly NAI generation characteristics and level concentrations evaluated, resemble to their world similar examples reviewed. The values ranging between 2100 to 156000 NAI/cm 3 implies all locations chosen as potential NTF at invigorating environment, clean fresh air atmosphere, satisfactory tourism air quality index, market biological effects to health resort programs and psychological benefits. Just cases destinations 1, 2, 3 and 4 endue ionic density (>15000 NAI/cm 3) possibly used for air ionotherapy treatments, including medical specialties: neurology, rheumatology, dermatology, pneumology and immunology.