Measuring quantitative proteomic distance between Spanish beef breeds

Abstract

Estimates of quantitative proteomic distance between populations have not been reported to date. Here, quantitative proteomic distances between three Spanish bovine breeds (Asturiana de los Valles, AV; Retinta, RE; and Rubia Gallega, RG) were estimated from two-dimensional electrophoresis profiles of meat samples of longissimus thoracis muscle at 2 h post-mortem. Statistically significant distances were detected between AV/RG and the most genetically different RE breed, using the novel QD measure of quantitative proteomic distance. In total, 18 differentially abundant myofibrillar and sarcoplasmic proteins/isoforms contributing to proteomic distances between breeds were confidently identified by tandem mass spectrometry. The fast skeletal myosin regulatory light chain 2 followed by other five interacting proteins exhibited the most pronounced relative change between breeds. In addition, most differentially represented proteins could be associated with variations in meat tenderness. Therefore, they could be candidate biomarkers for molecular breeding programs and authentication of the three Spanish beef breeds.

Assessment of Stress by Serum Biomarkers in Calves and Their Relationship to Ultimate pH as an Indicator of Meat Quality

“…RE calves showed the highest cortisol value at slaughter time, and even though we have not measured “temperament” as a feature in this research, unlike the aforementioned authors [ 47 ] have, it is well-known that the RE breed has a wilder “temperament” than AV and RG breeds, a factor that could explain this finding. However, the AV and RG breeds are improved breeds that provide easier handling and are genetically closer [ 48 ], and show the lowest cortisol levels at slaughter time.…”

“…The different temperaments of the breeds under study should be noted, as was indicated above for the RE breed. In contrast, the AV and RG breeds are genetically closer [ 48 ], and their temperament is calmer, non-aggressive or reactive, particularly the RG breed [ 50 ]. Other authors [ 8 , 47 ] indicated the importance of breed on stress response, but to the best of our knowledge, there is a lack of information concerning the effect of the interaction between the farm management system and the breed on the pre-slaughter stress biomarkers.…”

Comparative Proteomics of Potato Cultivars with a Variable Dormancy Period

“…The quantitation of statistically significant changes of protein abundance ( p value < 0.05) between potato cultivars was assessed by FC and RC statistics ( Table 3 ). It can be seen that FC is less useful than RC as a general measure of quantitative changes of proteins among sample groups, as previously shown [ 39 , 42 , 57 , 58 ]. The FC measure takes values of −∞ or +∞ for unshared (or unique) protein spots among sample groups, whatever their difference in volume.…”

Application of 2-D DIGE to study the effect of ageing on horse meat myofibrillar sub-proteome

“…1A vs. 1B). Therefore, 2-D DIGE would be an advantageous alternative to study and compare the effect of ageing in meat from different breeds or muscles, where a higher inter-sample variability would be expected due to intrinsic proteome differences among breeds and muscles [31,32].…”